| SAKABA Takeshi |  |
| Graduate School of Brain Science | |
| Professor |
Last Updated :2025/04/27
Researcher Profile and Settings
researchmap
Research funding number
80609511Research Interests
Research Areas
Research Experience
Published Papers
- Distinct release properties of glutamate/GABA co-transmission serve as a frequency-dependent filtering of supramammillary inputs
Himawari Hirai; Kohtarou Konno; Miwako Yamasaki; Masahiko Watanabe; Takeshi Sakaba; Yuki Hashimotodani
eLife, eLife Sciences Publications, Ltd, 13 RP99711 , 16 Dec. 2024, Scientific journal - Single-vesicle imaging reveals actin-dependent spatial restriction of vesicles at the active zone, essential for sustained transmission
Takafumi Miki; Yuji Okamoto; Miyuki Ueno-Umegai; Rio Toyofuku; Shun Hattori; Takeshi Sakaba
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, 121(43) e2402152121 , 15 Oct. 2024, Scientific journal - Increased vesicle fusion competence underlies long-term potentiation at hippocampal mossy fiber synapses
Ryota Fukaya; Himawari Hirai; Hirokazu Sakamoto; Yuki Hashimotodani; Kenzo Hirose; Takeshi Sakaba
Science Advances, American Association for the Advancement of Science (AAAS), 9(8) eadd3616 , 22 Feb. 2023, Scientific journal - Subcortical glutamatergic inputs exhibit a Hebbian form of long-term potentiation in the dentate gyrus
Himawari Hirai; Takeshi Sakaba; Yuki Hashimotodani
Cell Reports, Elsevier BV, 41(13) 111871 - 111871, Dec. 2022, Scientific journal - Excitatory selective LTP of supramammillary glutamatergic/GABAergic cotransmission potentiates dentate granule cell firing
Eri Tabuchi; Takeshi Sakaba; Yuki Hashimotodani
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, 119(13) e2119636119 , 29 Mar. 2022, Scientific journal - Rapid Ca2+ channel accumulation contributes to cAMP-mediated increase in transmission at hippocampal mossy fiber synapses
Ryota Fukaya; Marta Maglione; Stephan J. Sigrist; Takeshi Sakaba
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, 118(9) e2016754118 - e2016754118, 02 Mar. 2021, Scientific journal - Direct imaging of rapid tethering of synaptic vesicles accompanying exocytosis at a fast central synapse
Takafumi Miki; Mitsuharu Midorikawa; Takeshi Sakaba
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, 117(25) 14493 - 14502, 23 Jun. 2020, Scientific journal - Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V.
Cell Reports, 30(2) 409 - 420, Jan. 2020, Scientific journal - Developmental changes in the excitatory short-term plasticity at input synapses in the rat inferior colliculus.
Kitagawa M; Sakaba T
The European journal of neuroscience, 50(5) 2830 - 2846, Sep. 2019 - Ca-dependence of synaptic vesicle exocytosis and endocytosis at the hippocampal mossy fibre terminal.
Miyano R; Miki T; Sakaba T
The Journal of physiology, 597(16) 4373 - 4386, Aug. 2019 - Optimal dissection of a model circuit.
Sakaba T
The Journal of physiology, 596(20) 4807 - 4808, Oct. 2018 - Hapln4/Bral2 is a selective regulator for formation and transmission of GABAergic synapses between Purkinje and deep cerebellar nuclei neurons.
Edamatsu M; Miyano R; Fujikawa A; Fujii F; Hori T; Sakaba T; Oohashi T
Journal of neurochemistry, Aug. 2018 - Kinetics of transmitter release at the calyx of Held synapse.
Sakaba T
Proceedings of the Japan Academy. Series B, Physical and biological sciences, 94(3) 139 - 152, Mar. 2018 - Kinetics of Releasable Synaptic Vesicles and Their Plastic Changes at Hippocampal Mossy Fiber Synapses
Mitsuharu Midorikawa; Takeshi Sakaba
NEURON, CELL PRESS, 96(5) 1033 - +, Dec. 2017, Scientific journal - Fast Ca2+ Buffer-Dependent Reliable but Plastic Transmission at Small CNS Synapses Revealed by Direct Bouton Recording
Shin-ya Kawaguchi; Takeshi Sakaba
CELL REPORTS, CELL PRESS, 21(12) 3338 - 3345, Dec. 2017, Scientific journal - Synaptic Vesicle Endocytosis Occurs on Multiple Timescales and Is Mediated by Formin-Dependent Actin Assembly
Tolga Soykan; Natalie Kaempf; Takeshi Sakaba; Dennis Vollweiter; Felix Goerdeler; Dmytro Puchkov; Natalia L. Kononenko; Volker Haucke
NEURON, CELL PRESS, 93(4) 854 - +, Feb. 2017, Scientific journal - Distinct modes of endocytotic presynaptic membrane and protein uptake at the calyx of Held terminal of rats and mice
Yuji Okamoto; Noa Lipstein; Yunfeng Hua; Kun-Han Lin; Nils Brose; Takeshi Sakaba; Mitsuhara Midorikawa
ELIFE, ELIFE SCIENCES PUBLICATIONS LTD, 5, May 2016, Scientific journal - Ca(2+) current facilitation determines short-term facilitation at inhibitory synapses between cerebellar Purkinje cells.
Díaz-Rojas F; Sakaba T; Kawaguchi SY
The Journal of physiology, 593(22) 4889 - 4904, Nov. 2015 - Imaging Exocytosis of Single Synaptic Vesicles at a Fast CNS Presynaptic Terminal.
Midorikawa M; Sakaba T
Neuron, 88(3) 492 - 498, Nov. 2015 - Control of inhibitory synaptic outputs by low excitability of axon terminals revealed by direct recording.
Kawaguchi SY; Sakaba T
Neuron, 85(6) 1273 - 1288, Mar. 2015 - KV 10.1 opposes activity-dependent increase in Ca²⁺ influx into the presynaptic terminal of the parallel fibre-Purkinje cell synapse.
Mortensen LS; Schmidt H; Farsi Z, Barrantes-Freer A; Rubio ME; Ufartes R; Eilers J; Sakaba T; Stühmer W; Pardo LA
The Journal of physiology, 593(1) 181 - 196, Jan. 2015 - Developmental changes in Ca2+ channel subtypes regulating endocytosis at the calyx of Held
Mitsuharu Midorikawa; Yuji Okamoto; Takeshi Sakaba
JOURNAL OF PHYSIOLOGY-LONDON, WILEY-BLACKWELL, 592(16) 3495 - 3510, Aug. 2014, Scientific journal - Dynamic control of synaptic vesicle replenishment and short-term plasticity by Ca(2+)-calmodulin-Munc13-1 signaling.
Lipstein N; Sakaba T; Cooper BH; Lin KH; Strenzke N; Ashery U; Rhee JS; Taschenberger H; Neher E; Brose N
Neuron, 1, 79 82 - 96, Jul. 2013 - Fast neurotransmitter release regulated by the endocytic scaffold intersectin.
Sakaba T; Kononenko NL; Bacetic J; Pechstein A; Schmoranzer J; Yao L; Barth H; Shupliakov O; Kobler O; Aktories K; Haucke V
Proceedings of the National Academy of Sciences of the United States of America, 20, 110 8266 - 8271, May 2013 - Readily releasable pool of synaptic vesicles measured at single synaptic contacts.
Trigo FF; Sakaba T; Ogden D; Marty A
Proceedings of the National Academy of Sciences of the United States of America, 44, 109 18138 - 18143, Oct. 2012 - Activity-dependent modulation of endocytosis by calmodulin at a large central synapse.
Yao L; Sakaba T
Proceedings of the National Academy of Sciences of the United States of America, 1, 109 291 - 296, Jan. 2012 - cAMP modulates intracellular Ca2+ sensitivity of fast-releasing synaptic vesicles at the calyx of Held synapse.
Yao L; Sakaba T
Journal of neurophysiology, 6, 104 3250 - 3260, Dec. 2010 - Multivesicular release differentiates the reliability of synaptic transmission between the visual cortex and the somatosensory cortex.
Huang CH; Bao J; Sakaba T
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36, 30 11994 - 12004, Sep. 2010 - Target-dependent feedforward inhibition mediated by short-term synaptic plasticity in the cerebellum.
Bao J; Reim K; Sakaba T
The Journal of neuroscience : the official journal of the Society for Neuroscience, 24, 30 8171 - 8179, Jun. 2010 - Calcium dependence of exo- and endocytotic coupling at a glutamatergic synapse.
Hosoi N; Holt M; Sakaba T
Neuron, 2, 63 216 - 229, Jul. 2009 - Multiple roles of calcium ions in the regulation of neurotransmitter release.
Neher E; Sakaba T
Neuron, 6, 59 861 - 872, Sep. 2008 - Two Ca(2+)-dependent steps controlling synaptic vesicle fusion and replenishment at the cerebellar basket cell terminal.
Sakaba T
Neuron, 3, 57 406 - 419, Feb. 2008 - Quantitative analysis of calcium-dependent vesicle recruitment and its functional role at the calyx of Held synapse.
Hosoi N; Sakaba T; Neher E
The Journal of neuroscience : the official journal of the Society for Neuroscience, 52, 27 14286 - 14298, Dec. 2007 - The coupling between synaptic vesicles and Ca2+ channels determines fast neurotransmitter release.
Wadel K; Neher E; Sakaba T
Neuron, 4, 53 563 - 575, Feb. 2007 - Roles of the fast-releasing and the slowly releasing vesicles in synaptic transmission at the calyx of held.
Sakaba T
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22, 26 5863 - 5871, May 2006 - Distinct kinetic changes in neurotransmitter release after SNARE protein cleavage.
Sakaba T; Stein A; Jahn R; Neher E
Science (New York, N.Y.), 5733, 309 491 - 494, Jul. 2005 - Combining deconvolution and fluctuation analysis to determine quantal parameters and release rates.
Neher E; Sakaba T
Journal of neuroscience methods, 2, 130 143 - 157, Dec. 2003 - Direct modulation of synaptic vesicle priming by GABA(B) receptor activation at a glutamatergic synapse.
Sakaba T; Neher E
Nature, 6950, 424 775 - 778, Aug. 2003 - Involvement of actin polymerization in vesicle recruitment at the calyx of Held synapse.
Sakaba T; Neher E
The Journal of neuroscience : the official journal of the Society for Neuroscience, 3, 23 837 - 846, Feb. 2003 - Estimation of quantal parameters at the calyx of Held synapse.
Sakaba T; Schneggenburger R; Neher E
Neuroscience research, 4, 44 343 - 356, Dec. 2002 - Vesicle pools and short-term synaptic depression: lessons from a large synapse.
Schneggenburger R; Sakaba T; Neher E
Trends in neurosciences, 4, 25 206 - 212, Apr. 2002 - Estimating transmitter release rates from postsynaptic current fluctuations.
Neher E; Sakaba T
The Journal of neuroscience : the official journal of the Society for Neuroscience, 24, 21 9638 - 9654, Dec. 2001 - Calmodulin mediates rapid recruitment of fast-releasing synaptic vesicles at a calyx-type synapse.
Sakaba T; Neher E
Neuron, 6, 32 1119 - 1131, Dec. 2001 - Preferential potentiation of fast-releasing synaptic vesicles by cAMP at the calyx of Held.
Sakaba T; Neher E
Proceedings of the National Academy of Sciences of the United States of America, 1, 98 331 - 336, Jan. 2001 - Combining deconvolution and noise analysis for the estimation of transmitter release rates at the calyx of held.
Neher E; Sakaba T
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2, 21 444 - 461, Jan. 2001 - Quantitative relationship between transmitter release and calcium current at the calyx of held synapse.
Sakaba T; Neher E
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2, 21 462 - 476, Jan. 2001 - Submillisecond kinetics of glutamate release from a sensory synapse.
von Gersdorff H; Sakaba T; Berglund K; Tachibana M
Neuron, 5, 21 1177 - 1188, Nov. 1998 - Potentiation of transmitter release by protein kinase C in goldfish retinal bipolar cells.
Minami N; Berglund K; Sakaba T; Kohmoto H; Tachibana M
The Journal of physiology, 512 ( Pt 1) 219 - 225, Oct. 1998 - Two components of transmitter release in retinal bipolar cells: exocytosis and mobilization of synaptic vesicles.
Sakaba T; Tachibana M; Matsui K; Minami N
Neuroscience research, 4, 27(4) 357 - 370, Apr. 1997 - Ca2+ -activated K+ current at presynaptic terminals of goldfish retinal bipolar cells.
Sakaba T; Ishikane H; Tachibana M
Neuroscience Research, 27(3) 219 - 228, Mar. 1997 - Potentiation of Ca2+ transients in the presynaptic terminals of goldfish retinal bipolar cells.
Kobayashi K; Sakaba T; Tachibana M
J. Physiol., 482 7 - 13, Jan. 1995
Books etc
- 顆粒放出機構（特集 シナプス）
三木 崇史; 坂場 武史
生体の科学, Jan. 2023, Contributor, 27-31 - Synaptic vesicle dynamics at the calyx of Held and other central synapses
Miki T; Hashimotodani Y; Sakaba T
IOP (Biophysical Society), Dec. 2022, Contributor, Chapter 13, Exocytosis: From Molecules to Cells - 中枢神経系におけるシナプス伝達の生理学（ブレインサイエンス・レビュー2018）
廣川信隆編集
ブレインサイエンス振興財団, Mar. 2018, Contributor - スタンフォード神経生物学 Liqun Luo著
柚崎通介; 岡部繁男監訳
メディカルインターナショナル, Aug. 2017, Joint translation, １０章 - Patch clamp techinques
Takeshi Sakaba; Akihiro Hazama; Toshio Maruyama; Edited by; Yasunobu Okada
Springer, 2012, Joint work, Patch clamp capacitance measurements
Presentations
- Kinetic analysis of transmitter release at hippocampal mossy fiber synapses
Takeshi Sakaba
FENS Satellite Symposium, Recent Advancements: Unraveling Synaptic Transmission In Hippocampal Mossy Fiber Synapses, ISTA, Austria, 24 Jun. 2024 - Electrophysiology of synaptic transmission (in Japanese)
Takeshi Sakaba
The 46th meeting of the Japanese Neuroscience Society, 03 Aug. 2023, Invited oral presentation - Synaptic vesicle pools at mammalian central synapses
Takeshi Sakaba
2021 Annual meeting of Korea Society for Brain and Neural Sciences (KSBNS), 21 May 2021 - Kinetics of transmitter release at hippocampal mossy fiber synapses
Takeshi Sakaba
Kinetics of transmitter release at hippocampal mossy fiber synapses, 06 Sep. 2019 - Comparison of the transmitter release properties between the calyx of Held synapse and mossy fiber-CA3 synapse
Takeshi Sakaba
Quantitaive aspects of membrane fuison and fission, Biopysical Society Thematic meetings, 08 May 2019, Invited oral presentation - Biophysics of neurotransmitter release at central synapses
Takeshi Sakaba
NIH neuroscience senminar series, 17 Dec. 2018 - Regulation of exocytosis by cAMP at hippocampal mossy fiber terminals.
Takeshi Sakaba
日本神経科学学会, 28 Jul. 2018 - パッチクランプ法をはじめとする電気生理学的計測法について（教育講演）
坂場武史
日本神経科学学会, 20 Jul. 2017 - 哺乳類中枢シナプス前終末の生理学的研究
坂場武史
日本生理学会（シンポジウム）, 28 Mar. 2017 - MECHANISM OF TRANSMITTER RELEASE AT THE CALYX OF HELD SYNAPSE
Takeshi Sakaba
MECHANISM OF TRANSMITTER RELEASE AT THE CALYX OF HELD SYNAPSE, 23 Mar. 2017 - 中枢神経系におけるシナプス伝達の生理学 （塚原賞記念講演）
坂場武史
日本神経科学学会, 20 Jul. 2016 - Physiology of axon and axon terminals
Takeshi Sakaba
Chinese Neuroscience Society meeting, 21 Sep. 2015 - Presynaptic recordings from an inhibitory terminal.
Takeshi Sakaba
Gordon Research Conference "Cell Biology of the Neuron", 24 Jun. 2014 - Exo-endocytotic coupling at the calyx of Held synapse.
Takeshi Sakaba
International society for neurochemistry meeting, 23 Apr. 2013 - short-term synaptic plasticity in cerebellum
Takeshi Sakaba
Scandinavian physiological Society meeting, Mar. 2010 - Rate-limiting steps in neurotransmitter releasse
Takeshi Sakaba
Slovenian physiological Society meeting, Nov. 2009 - Kinetics of transmitter release at the calyx of Held synapse
Takeshi Sakaba
Gordon Research Conference for Synaptic transmission, Jul. 2006
Research Projects
- 集団としての皮質シナプス機能を理解、操作するためのアプローチ構築
坂場 武史
日本学術振興会, 科学研究費助成事業, 2024/06 -2028/03, 挑戦的研究(開拓), 同志社大学 - 伝達物質放出部位ダイナミクスを軸としたシナプス可塑性の統一的理解
坂場 武史
日本学術振興会, 科学研究費助成事業, 2024/04 -2027/03, 基盤研究(B), 同志社大学 - Nanoscale visualization and mechanstic understanding of presynaptic plasticity
坂場 武史; 三木 崇史; 坂本 寛和
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), 2020/10 -2025/03, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Doshisha University - Regulation of hippocampal mossy fiber synapses and its functional significance.
坂場 武史
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), 2021/04 -2024/03, Grant-in-Aid for Scientific Research (B), Doshisha University - 海馬苔状線維シナプス・局所神経回路のスクラップ＆ビルド
坂場武史
文部科学省, 新学術領域研究(公募), 2019/04 -2021/03, Principal investigator, Competitive research funding - 哺乳類中枢シナプス伝達・短期可塑性メカニズムの解明
坂場武史
日本学術振興会, 基盤研究（B）, 2017/04 -2020/03, Principal investigator, Competitive research funding - 聴覚系シナプスのスクラップ＆ビルドによる回路機能制御
坂場武史
文部科学省, 新学術領域（公募）, 2017/04 -2019/03, Principal investigator, Competitive research funding - 軸索とシナプス前終末の生理学的研究
坂場武史
日本学術振興会, 基盤研究（B）, 2015/04 -2018/03, Principal investigator, Competitive research funding - シナプス小胞解析のための蛍光プローブの適用
坂場武史
日本学術振興会, 挑戦的萌芽研究, 2015/04 -2018/03, Principal investigator, Competitive research funding - 糖鎖によるシナプス前終末の機能修飾
坂場武史
文部科学省, 新学術領域（公募）, 2014/04 -2016/03, Principal investigator, Competitive research funding - 単一シナプス前終末の光刺激法の開発
坂場武史
日本学術振興会, 挑戦的萌芽研究, 2012/04 -2015/03, Principal investigator, Competitive research funding - 神経伝達物質放出における律速段階の解明
坂場武史
日本学術振興会, 基盤研究（B）, 2012/04 -2015/03, Principal investigator, Competitive research funding
