Researcher Profile and Settings

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https://researchmap.jp/yoshirotahara

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Select publications:

1. R. Hanaki, K. Harada*, Y. Sasaki, M. Matsumoto, Y. Tahara*, Stomatitis Healing via Hydrogels Comprising Proline, Carboxyvinyl Polymer, and Water., Gels, 11(2), 108 (2025).
2. H.A. Nancy, Y. Iwatsuki, K. Yabuuchi, S. Aso, T. Katsumata, K. Fukumoto, Y. Tanaka, T. Nakai, T. Shimoboji, M. Matsumoto, Y. Tahara*, Self-assembled nanogels based on hyaluronic acid for antibody protection from heat denaturation, Biochem. Eng. J., 196, 108959 (2023).
3. Y. Tahara, R. Mizuno, T. Nishimura, S.A. Mukai, R. Wakabayashi, N. Kamiya, K. Akiyoshi, M. Goto*, A solid-in-oil-in-water emulsion: An adjuvant-based immune-carrier enhances vaccine effect, Biomaterials, 282, 121385 (2022).
4. Y. Tahara, K. Morita, R. Wakabayashi, N. Kamiya, M. Goto*, Biocompatible Ionic Liquid Enhances Transdermal Antigen Peptide Delivery and Preventive Vaccination Effect, Mol. Pharm., 17(10) 3845 - 3856 (2020).
5. Y. Tahara, K. Akiyoshi*, Current advances in self-assembled nanogel delivery systems for immunotherapy, Adv. Drug Deliv. Rev., 95(1), 65–76 (2015).
6. Y. Tahara, S. Mukai, S. Sawada, Y. Sasaki, K. Akiyoshi*, Nanocarrier-integrated microspheres: Nanogel tectonic engineering for advanced drug delivery systems, Adv. Mater., 27(34), 5080-5088 (2015).
7. Y. Tahara, T. Kaneko, R. Toita, C. Yoshiyama, T. Kitaoka, T. Niidome, Y. Katayama, N. Kamiya, M. Goto*, A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells, J. Control. Release, 161(3), 713-721 (2012).
8. Y. Tahara, K. Namatsu, N. Kamiya, M. Hagimori, S. Kamiya, M. Arakawa, M.Goto*, Transcutaneous immunization by a solid-in-oil nanodispersion, Chem. Commun., 46(48), 9200-9202 (2010).
9. Y. Tahara, S. Honda, N. Kamiya, H. Piao, A.Hirata, E. Hayakawa, T. Fujii, M. Goto*, A solid-in-oil nanodispersion for transcutaneous protein delivery, J. Control. Release, 131(1), 14-18 (2008).

Research Interests

• Transdermal delivery
• Cancer immunotherapy
• Gel
• Tissue engineering
• Emulsion
• Polysaccharides
• Nanotechnology
• Vaccine
• Cancer
• Biomaterials
• Drug Delivery System

Research Areas

• Nanotechnology/Materials / Biochemistry
• Nanotechnology/Materials / Polymer chemistry
• Life sciences / Biomaterials
• Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering

Research Experience

• Doshisha University, Department of Chemical Engineering and Materials Science, Associate Professor (tenure), 2023/04 - Today
• Doshisha University, Department of Chemical Engineering and Materials Science, Associate professor (non tenure), 2019/04 - 2023/03
• Kyushu University, Japan, Department of Applied Chemistry, Post-droctoral researcher, 2017/03 - 2019/03
• JST-ERATO Akiyoshi Bio-Nanotransporter Project, Japan, Post-doctoral Researcher, 2012/04 - 2017/02
• Kyoto University, Japan. (Advisor: Prof. Kazunari Akiyoshi), Department of Polymer Chemistry, Post-doctoral Researcher, 2012/04 - 2017/02
• Japan Society for the Promotion of Science (JSPS) Research Fellow (DC1), 2009/04 - 2012/03

Education

• Kyushu University, Japan. (Advisor: Prof. Masahiro Goto & Prof. Noriho Kamiya), Department of Applied Chemistry, Ph.D., Biochemical Engineering, 2007/04 - 2012/03
• Kyushu University, Japan (Advisor: Prof. Masahiro Goto & Prof. Noriho Kamiya), Department of Applied Chemistry, B.S., Biochemical Engineering, 2003/04 - 2007/03

Degree

• Ph.D., Kyushu University

Awards

• Best Oral Presenter Award for Young Researchers, Japan Society of Drug Delivery System, Japan
  Jun. 2011
• Best Poster Presenter Award for Young Researchers, Division of Biochemical Enginnering, The Society of Chemical Engineers, Japan
  Sep. 2010
• Best Poster Presenter Award for Young Researchers, Japan Society of Drug Delivery System, Japan
  Jul. 2009
• Best Poster Presenter Award for Young Researchers, Division of Biochemical Enginnering, The Society of Chemical Engineers, Japan
  Sep. 2007
• Best Oral Presenter Award for Young Researchers, The Society of Chemical Engineers, Japan
  Jul. 2007

Published Papers

• Stomatitis Healing via Hydrogels Comprising Proline, Carboxyvinyl Polymer, and Water.
  Raichi Hanaki; Koji Harada; Yoshihiro Sasaki; Michiaki Matsumoto; Yoshiro Tahara
  Gels (Basel, Switzerland), 11(2), 03 Feb. 2025, Scientific journal
  

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• Methylglyoxal compromises callus mineralization and impairs fracture healing through suppression of osteoblast terminal differentiation.
  Tetsuya Seto; Kiminori Yukata; Shunya Tsuji; Yusuke Takeshima; Takeshi Honda; Akihiko Sakamoto; Kenji Takemoto; Hiroki Sakai; Mayu Matsuo; Yurika Sasaki; Mizuki Kaneda; Mikako Yoshimura; Atsushi Mihara; Kazuya Uehara; Aira Matsugaki; Takayoshi Nakano; Koji Harada; Yoshiro Tahara; Keiko Iwaisako; Ryoji Yanai; Norihiko Takeda; Takashi Sakai; Masataka Asagiri
  Biochemical and biophysical research communications, 747 151312 - 151312, 02 Feb. 2025, Scientific journal
  

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• Effect of Lipase Ionic Liquid Coating and Imprinting on Transesterification and Thermostability
  Michiaki Matsumoto; Yoshiro Tahara
  Chemical and biochemical engineering quarterly, Croatian Society of Chemical Engineers/HDKI, 38(4) 313 - 318, 20 Jan. 2025, Scientific journal
  

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• Fabrication, characterization, and adsorption studies of thermally modified peanut shell charcoal for Orange G dye removal
  Asif Ali; Shah Rukh Khan; Muffarih Shah; Michiaki Matsumoto; Yoshiro Tahara; Sheraz Muhammad; Shahzad Khan
  Biomass Conversion and Biorefinery, 24 Dec. 2024, Scientific journal
  

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• Novel Injectable Collagen/Glycerol/Pullulan Gel Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and the Repair of Rat Cranial Defects.
  Xin Wang; Satoshi Komasa; Yoshiro Tahara; Shihoko Inui; Michiaki Matsumoto; Kenji Maekawa
  Gels (Basel, Switzerland), 10(12), 28 Nov. 2024, Scientific journal
  

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• Separation of Lactic Acid and Acetic Acid through Supported Ionic Liquid Membranes Containing Cyphos IL 101
  Michiaki MATSUMOTO; Hiroto FUKADA; Yoshiro TAHARA
  Solvent Extraction Research and Development, Japan, Japan Association of Solvent Extraction, 31(2) 91 - 95, Apr. 2024, Scientific journal
  

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• Cholesterol -Bearing Polysaccharide-Based Nanogels for Development of Novel Immunotherapy and Regenerative Medicine
  Tetsuya Adachi; Yoshiro Tahara; Kenta Yamamoto; Toshiro Yamamoto; Narisato Kanamura; Kazunari Akiyoshi; Osam Mazda
  Gels, 10(3) 206 - 206, Mar. 2024, Scientific journal
  

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• A stable, non-emulsifying, regenerable ionic liquid-based extraction system for safe removal of perfluorooctanoic acid from water
  Mariusz GRABDA; Michiaki MATSUMOTO; Yoshiro TAHARA; Maciej ZAWADZKI; Toma NAKANO; Sylwia OLESZEK
  Journal of Environmental Chemical Engineering, Elsevier BV, 12(2) 112320 - 112320, Feb. 2024, Scientific journal
  

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• Enhanced Esterification Activity and Thermostability of Imprinted Poly(Ethylene Glycol)-Lipase Complex
  M. Matsumoto; Yoshiro Tahara
  Chemical and Biochemical Engineering Quarterly, Croatian Society of Chemical Engineers/HDKI, 37(4) 255 - 260, 18 Jan. 2024, Scientific journal
  

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• Extraction of Organic Acids with Hydrophobic Eutectic Mixtures Containing Terpenoids and Decanoic Acid
  Michiaki Matsumoto; Makoto Ueda; Yoshiro Tahara
  Journal of Chemical Engineering of Japan, Informa UK Limited, 56(1) 2277827 , 08 Nov. 2023, Scientific journal
  

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• Self-assembled nanogels based on hyaluronic acid for antibody protection from heat denaturation
  Haddad Antonia-Nancy; Yuto Iwatsuki; Kohei Yabuuchi; Shogo Aso; Toru Katsumata; Keisuke Fukumoto; Yurika Tanaka; Takashi Nakai; Tsuyoshi Shimoboji; Michiaki Matsumoto; Yoshiro Tahara
  BIOCHEMICAL ENGINEERING JOURNAL, ELSEVIER, 196 108955 - 108959, Jul. 2023, Scientific journal
  

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• Specific Extraction of Aspartic Acid and Tyrosine with Aqueous Two-phase Systems Formed by Hydrophilic Organic Solvent and Monomeric Sugars
  Michiaki Matsumoto; Asuka Wada; Yoshiro Tahara
  Journal of Chemical Engineering of Japan, Informa UK Limited, 56(1) 2220550 , 15 Jun. 2023, Scientific journal
  

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• Up-Hill Transport of Tryptophan through Polymer Inclusion Membrane Containing Aliquat 336
  Michiaki MATSUMOTO; Noritake KITA; Yoshiro TAHARA
  Solvent Extraction Research and Development, Japan, Japan Association of Solvent Extraction, 30(2) 177 - 181, 26 May 2023, Scientific journal
  

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• Partition of Amino Acids in Aqueous Two-Phase Systems Comprising Hydrophilic Organic Solvents and Salts
  Michiaki Matsumoto; Takehiro Sato; Asuka Wada; Yoshiro Tahara
  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, Society of Chemical Engineers, Japan, 55(11) 344 - 348, 20 Nov. 2022, Scientific journal
  

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• Three-Dimensional Culture of Cartilage Tissue on Nanogel-Cross-Linked Porous Freeze-Dried Gel Scaffold for Regenerative Cartilage Therapy: A Vibrational Spectroscopy Evaluation.
  Tetsuya Adachi; Nao Miyamoto; Hayata Imamura; Toshiro Yamamoto; Elia Marin; Wenliang Zhu; Miyuki Kobara; Yoshihiro Sowa; Yoshiro Tahara; Narisato Kanamura; Kazunari Akiyoshi; Osam Mazda; Ichiro Nishimura; Giuseppe Pezzotti
  International journal of molecular sciences, 23(15) 8099 , 01 Aug. 2022, Scientific journal
  

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• Glycine-Mediated Paclitaxel Dispersion in a Phospholipid Complex
  Yoshiro Tahara; Ueyama Kenichi; Matsumoto Michiaki
  Journal of Chemical Engineering of Japan, The Society of Chemical Engineers, Japan, 55(6) 225 - 229, 20 Jun. 2022
  

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• Removal of Perfluorooctanoic Acid from Water Using a Hydrophobic Ionic Liquid Selected Using the Conductor-like Screening Model for Realistic Solvents
  Mariusz Grabda; Maciej Zawadzki; Sylwia Oleszek; Michiaki Matsumoto; Marek Królikowski; Yoshiro Tahara
  Environmental Science & Technology, American Chemical Society (ACS), 56(10) 6445 - 6454, 17 May 2022, Scientific journal
  

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• Extraction of 1,3-Propanediol with Aqueous Two-Phase Systems Formed by Protic Ionic Liquids and Inorganic Salts
  Michiaki MATSUMOTO; Fumiya SHINJI; Yoshiro TAHARA
  Solvent Extraction Research and Development, Japan, Japan Association of Solvent Extraction, 29(2) 67 - 71, 25 Apr. 2022, Scientific journal
  

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• A solid-in-oil-in-water emulsion: An adjuvant-based immune-carrier enhances vaccine effect
  Yoshiro Tahara; Riko Mizuno; Tomoki Nishimura; Sada-atsu Mukai; Rie Wakabayashi; Noriho Kamiya; Kazunari Akiyoshi; Masahiro Goto
  Biomaterials, Elsevier BV, 282 121385 - 121385, Mar. 2022, Scientific journal
  

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• Adsorption of metallic ions on amidoxime-chitosan/ cellulose hydrogels
  Takaaki Tatsumi; Yoshiro Tahara; Michiaki Matsumoto
  Separations, 8(11), Nov. 2021, Scientific journal
  

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• Extraction of proteins with aqueous two-phase systems formed by protic ionic liquids and inorganic salts
  Michiaki Matsumoto; Fumiya Shinji; Yoshiro Tahara
  Solvent Extraction Research and Development, 28(2) 141 - 148, 06 Jun. 2021, Scientific journal
  

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• Effects of imprinting and water activity on transesterification and thermostability with lipases in ionic liquid
  M. Matsumoto; K. Nakao; Y. Tahara
  Chemical and Biochemical Engineering Quarterly, (1) 57 - 63, 10 Feb. 2021, Scientific journal
  

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• Permeation of Phenylalanine through Polymer Inclusion Membrane Containing Bi-functional Ionic Liquid
  Michiaki Matsumoto; Kei Inoue; Yoshiro Tahara
  Solvent Extraction Research and Development, 27(2) 143 - 147, 11 Dec. 2020, Scientific journal
  

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• Solid-in-oil nanodispersions as a novel delivery system to improve the oral bioavailability of bisphosphate, risedronate sodium
  Yanting Hou; Hongyu Piao; Yoshiro Tahara; Shouhong Qin; Jingying Wang; Qingliang Kong; Meijuan Zou; Gang Cheng; Masahiro Goto
  European Journal of Pharmaceutical Sciences, Elsevier BV, 155(1) 105521 - 105521, 01 Dec. 2020, Scientific journal
  

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• Biocompatible Ionic Liquid Enhances Transdermal Antigen Peptide Delivery and Preventive Vaccination Effect', Molecular Pharmaceutics
  Yoshiro Tahara; Kaho Morita; Rie Wakabayashi; Noriho Kamiya; Masahiro Goto
  Molecular Pharmaceutics, 17(10) 3845 - 3856, 05 Oct. 2020
  

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• In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging
  Tetsuya Adachi; Francesco Boschetto; Nao Miyamoto; Toshiro Yamamoto; Elia Marin; Wenliang Zhu; Narisato Kanamura; Yoshiro Tahara; Kazunari Akiyoshi; Osam Mazda; Ichiro Nishimura; Giuseppe Pezzotti
  Materials, MDPI AG, 13(19) 4275 - 4275, 01 Oct. 2020, Scientific journal
  

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• Lactic Acid Permeation through Deep Eutectic Solvents-Based Polymer Inclusion Membranes
  Michiaki Matsumoto; Sae Takemori; Yoshiro Tahara
  Membranes, MDPI AG, 10(9) 244 - 244, 19 Sep. 2020, Scientific journal
  

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• Choline and amino acid based biocompatible ionic liquid mediated transdermal delivery of the sparingly soluble drug acyclovir
  Md. Rafiqul Islam; Md. Raihan Chowdhury; Rie Wakabayashi; Yoshiro Tahara; Noriho Kamiya; Muhammad Moniruzzaman; Masahiro Goto
  International Journal of Pharmaceutics, Elsevier BV, 582 119335 - 119335, 30 May 2020, Scientific journal
  

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• Extraction of Rare Earth Metal Ions with an Undiluted Hydrophobic Pseudoprotic Ionic Liquid
  Michiaki Matsumoto; Takuya Yamaguchi; Yoshiro Tahara
  Metals, MDPI AG, 10(4) 502 - 502, 11 Apr. 2020, Scientific journal
  

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• A Solid-in-Oil Nanodispersion System for Transcutaneous Immunotherapy of Cow's Milk Allergies.
  Momoko Kitaoka; Wei Xiao; Qingliang Kong; Yoshiro Tahara; Noriho Kamiya; Masahiro Goto
  Pharmaceutics, 12(3) 205 , Mar. 2020, Scientific journal
  

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• Solid-in-Oil Nanodispersions for Transcutaneous Immunotherapy of Japanese Cedar Pollinosis.
  Qingliang Kong; Momoko Kitaoka; Rie Wakabayashi; Yoshiro Tahara; Noriho Kamiya; Masahiro Goto
  Pharmaceutics, 12(3) 240 , Mar. 2020, Scientific journal
  

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• Transcutaneous Cancer Vaccine Using a Reverse Micellar Antigen Carrier
  Shuto Kozaka, Yoshiro Tahara, Rie Wakabayashi, Tahakhiro, Nakata, Taro Ueda, Noriho Kamiya, Masahiro Goto
  Molecular Pharmaceutics, 17(2) 645 - 655, 03 Feb. 2020
  

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• Ionic liquids with N-methyl-2-pyrrolidonium cation as an enhancer for topical drug delivery: Synthesis, characterization, and skin-penetration evaluation
  Rahman Md. Moshikur, Md. Raihan Chowdhury, Rie Wakabayashi, Yoshiro Tahara, Noriho Kamiya, Muhammad Moniruzzaman, Masahiro Goto
  Journal of Molecular Liquids, 299(1) 112166 , 01 Feb. 2020, Scientific journal
  

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• Solid-in-oil nanodispersions for intranasal vaccination: Enhancement of mucosal and systemic immune responses
  Qingliang Kong, Momoko Kitaoka, Yoshiro Tahara, Rie Wakabayashi, Noriho Kamiya, Masahiro Goto
  International Journal of Pharmaceutics, 572(15) 118777 , 15 Dec. 2019
  

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• Transcutaneous delivery of immunomodulating pollen extract-galactomannan conjugate by solid-in-oil nanodispersions for pollinosis immunotherapy
  Qingliang Kong, Kouki Higasijima, Rie Wakabayashi, Yoshiro Tahara, Momoko Kitaoka, Hiroki Obayashi, Yanting Hou, Noriho Kamiya, Masahiro Goto
  Pharmaceutics, 11(11) 563 , Nov. 2019
  

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• Synthesis and characterization of choline–fatty-acid-based ionic liquids: A new biocompatible surfactant
  Md Korban Ali; Rahman Md Moshikur; Rie Wakabayashi; Yoshiro Tahara; Muhammad Moniruzzaman; Noriho Kamiya; Masahiro Goto
  Journal of Colloid and Interface Science, 551(1) 72 - 80, 01 Sep. 2019
  

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• Development of a novel ionic liquid-curcumin complex to enhance its solubility, stability, and activity
  Md. Raihan Chowdhury; Rahman Md Moshikur; Rie Wakabayashi; Yoshiro Tahara; Noriho Kamiya; Muhammad Moniruzzaman; Masahiro Goto
  Chemical Communications, 55 7737 - 7740, 11 Jul. 2019
  

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• In vivo biocompatibility, pharmacokinetics, antitumor efficacy, and hypersensitivity evaluation of ionic liquid-mediated paclitaxel formulations
  Md. Raihan Chowdhury; Rahman Md Moshikur; Rie Wakabayashi; Yoshiro Tahara; Noriho Kamiya; Muhammad Moniruzzaman; Masahiro Goto
  International Journal of Pharmaceutics, 565 219 - 226, 30 Jun. 2019
  

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• Osteogenic response of mesenchymal progenitor cells to natural polysaccharide nanogel and atelocollagen scaffolds: A spectroscopic study
  Satoshi Horiguchi; Tetsuya Adachi; Alfredo Rondinella; Francesco Boschetto; Elia Marin; Wenliang Zhu; Yoshiro Tahara; Toshiro Yamamoto; Narisato Kanamura; Kazunari Akiyoshi; Giuseppe Pezzotti; Osam Mazda
  Materials Science and Engineering C, 99 1325 - 1340, Jun. 2019, Scientific journal
  

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• Transcutaneous Codelivery of Tumor Antigen and Resiquimod in Solid-in-Oil Nanodispersions Promotes Antitumor Immunity
  Rie Wakabayashi; Hidetoshi Kono; Shuto Kozaka; Yoshiro Tahara; Noriho Kamiya; Masahiro Goto
  ACS Biomaterials Science & Engineering, 5(5) 2297 - 2306, 13 May 2019
  

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• Ionic liquids with methotrexate moieties as a potential anticancer prodrug: Synthesis, characterization and solubility evaluation
  Rahman Md. Moshikur; Md. Raihan Chowdhury; Rie Wakabayashi; Yoshiro Tahara; Muhammad Moniruzzaman; Masahiro Goto
  Journal of Molecular Liquids, 278(15) 226 - 233, 15 Mar. 2019
  

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• Antigen Delivery Targeting Tumor-Associated Macrophages Overcomes Tumor Immune Resistance
  Daisuke Muraoka; Naohiro Seo; Tae Hayashi; Yoshiro Tahara; Keisuke Fujii; Isao Tawara; Yoshihiro Miyahara; Kana Okamori; Hideo Yagita; Seiya Imoto; Rui Yamaguchi; Mitsuhiro Komura; Satoru Miyano; Masahiro Goto; Shin-ichi Sawada; Akira Asai; Hiroaki Ikeda; Kazunari Akiyoshi; Naozumi Harada; Hiroshi Shiku
  Journal of Clinical Investigation, 129(3) 1278 - 1294, 01 Mar. 2019
  

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• Structural effects and lymphocyte activation properties of self-assembled polysaccharide nanogels for effective antigen delivery
  Risako Miura; Yoshiro Tahara; Shin-ichi Sawada; Yoshihiro Sasaki; Kazunari Akiyoshi
  Scientific Reports, NATURE PUBLISHING GROUP, 8 16464 - 16472, Nov. 2018, Scientific journal
  

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• Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration
  Yoshiki Sato; Kenta Yamamoto; Satoshi Horiguchi; Yoshiro Tahara; Kei Nakai; Shin-ichiro Kotani; Fumishige Oseko; Giuseppe Pezzotti; Toshiro Yamamoto; Tsunao Kishida; Narisato Kanamura; Kazunari Akiyoshi; Osam Mazda
  Scientific Reports, NATURE PUBLISHING GROUP, 8 15824 - 15833, Oct. 2018, Scientific journal
  

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• Characterization and cytotoxicity evaluation of biocompatible amino acid esters used to convert salicylic acid into ionic liquids
  Rahman Md. Moshikur; Md. Raihan Chowdhury; Rie Wakabayashi; Yoshiro Tahara; Muhammad Moniruzzaman; Masahiro Goto
  International Journal of Pharmaceutics, Elsevier B.V., 546(1-2) 31 - 38, 30 Jul. 2018, Scientific journal
  

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• Ionic-Liquid-Based Paclitaxel Preparation: A New Potential Formulation for Cancer Treatment
  Md. Raihan Chowdhury, Rahman; Md. Moshikur; Rie Wakabayashi; Yoshiro Tahara; Noriho Kamiya; Muhammad Moniruzzaman; Masahiro Goto
  Molecular Pharmaceutics, 15(6) 2484 - 2488, 06 Jun. 2018
  

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• Mechanistic investigation of transcutaneous protein delivery using solid-in-oil nanodispersion: A case study with phycocyanin.
  Safrina Dyah Hardiningtyas; Rie Wakabayashi; Momoko Kitaoka; Yoshiro Tahara; Kosuke Minamihata; Masahiro Goto; Noriho Kamiya
  European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 127(0) 44 - 50, Jun. 2018, Scientific journal
  

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• Solid-in-Oil Peptide Nanocarriers for Transcutaneous Cancer Vaccine Delivery against Melanoma
  Rie Wakabayashi; Masato Sakuragi; Shuto Kozaka; Yoshiro Tahara; Noriho Kamiya; Masahiro Goto
  Molecular Pharmaceutics, 15(3) 955 - 961, 05 May 2018
  

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• Genipin-stabilized caseinatehitosan nanoparticles for enhanced stability and anti-cancer activity of curcumin
  Muhamad Alif Razi; Rie Wakabayashi; Yoshiro Tahara; Masahiro Goto; Noriho Kamiya
  Colloids and Surfaces B: Biointerfaces, Elsevier B.V., 164(1) 308 - 315, 01 Apr. 2018, Scientific journal
  

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• Activated CD8+ T Cell Extracellular Vesicles Prevent Tumour Progression by Targeting of Lesional Mesenchymal Cells
  Naohiro Seo; Yoshitaka Shirakura; Yoshiro Tahara; Fumiyasu Momose; Naozumi Harada; Hiroaki Ikeda; Kazunari Akiyoshi; Hiroshi Shiku
  Nature Communications, NATURE PUBLISHING GROUP, 9(0) 435 , Jan. 2018, Scientific journal
  

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• Glycan profiling analysis using evanescent-field fluorescence-assisted lectin array: Importance of sugar recognition for cellular uptake of exosomes from mesenchymal stem cells
  Asako Shimoda; Yoshiro Tahara; Shin-ichi Sawada; Yoshihiro Sasaki; Kazunari Akiyoshi
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC ELSEVIER SCIENCE, 491(3) 701 - 707, Sep. 2017, Scientific journal
  

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• Self-Assembled Nanogels of Cholesterol-Bearing Hydroxypropyl Cellulose: A Thermoresponsive Building Block for Nanogel Tectonic Materials
  Yoshiro Tahara; Mizuki Sakiyama; Shigeo Takeda; Tomoki Nishimura; Sada-atsu Mukai; Shin-ichi Sawada; Yoshihiro Sasaki; Kazunari Akiyoshi
  LANGMUIR, AMER CHEMICAL SOC, 32(47) 12283 - 12289, Nov. 2016, Scientific journal
  

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• Current advances in self-assembled nanogel delivery systems for immunotherapy
  Yoshiro Tahara; Kazunari Akiyoshi
  ADVANCED DRUG DELIVERY REVIEWS, ELSEVIER SCIENCE BV, 95(1) 65 - 76, Dec. 2015
  

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• Nanocarrier-Integrated Microspheres: Nanogel Tectonic Engineering for Advanced Drug-Delivery Systems
  Yoshiro Tahara; Sada-atsu Mukai; Shin-ichi Sawada; Yoshihiro Sasaki; Kazunari Akiyoshi
  ADVANCED MATERIALS, WILEY-V C H VERLAG GMBH, 27(34) 5080 - 5088, Sep. 2015, Scientific journal
  

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• Effective CpG DNA delivery using amphiphilic cycloamylose nanogels
  Y. Tahara; J. Yasuoka; S. Sawada; Y. Sasaki; K. Akiyoshi
  BIOMATERIALS SCIENCE, ROYAL SOC CHEMISTRY, 3(2) 256 - 264, 01 Feb. 2015, Scientific journal
  

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• Nanogel-Based Immunologically Stealth Vaccine Targets Macrophages in the Medulla of Lymph Node and Induces Potent Antitumor Immunity
  Daisuke Muraoka; Naozumi Harada; Tae Hayashi; Yoshiro Tahara; Fumiyasu Momose; Shin-ichi Sawada; Sada-atsu Mukai; Kazunari Akiyoshi; Hiroshi Shiku
  ACS NANO, AMER CHEMICAL SOC, 8(9) 9209 - 9218, Sep. 2014, Scientific journal
  

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• Sucrose laurate-enhanced transcutaneous immunization with a solid-in-oil nanodispersion
  Momoko Kitaoka; Kana Imamura; Yuya Hirakawa; Yoshiro Tahara; Noriho Kamiya; Masahiro Goto
  MEDCHEMCOMM, ROYAL SOC CHEMISTRY, 5(1) 20 - 24, 01 Jan. 2014, Scientific journal
  

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• Needle-free immunization using a solid-in-oil nanodispersion enhanced by a skin-permeable oligoarginine peptide
  Momoko Kitaoka; Kana Imamura; Yuya Hirakawa; Yoshiro Tahara; Noriho Kamiya; Masahiro Goto
  INTERNATIONAL JOURNAL OF PHARMACEUTICS, ELSEVIER SCIENCE BV, 458(2) 334 - 339, Dec. 2013, Scientific journal
  

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• Cationic amphiphilic polysaccharide nanoballs: protein stabilization and intracellular delivery by nano-encapsulation
  Haruko Takahashi; Yoshiro Tahara; Shin-ichi Sawada; Kazunari Akiyoshi
  BIOMATERIALS SCIENCE, ROYAL SOC CHEMISTRY, 1(8) 842 - 849, 01 Aug. 2013, Scientific journal
  

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• Nanogel bottom-up gel biomaterials for protein delivery: Photopolymerization of an acryloyl-modified polysaccharide nanogel macromonomer
  Yoshiro Tahara; Satoshi Kosuge; Shin-Ichi Sawada; Yoshihiro Sasaki; Kazunari Akiyoshi
  Reactive and Functional Polymers, 73(7) 958 - 964, Jul. 2013, Scientific journal
  

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• Preparation of Multiple Emulsions to Depress the Rlease of Drugs and Enhanced Permeation Effect in Transdermal Delivery
  Yuko Abe; Yoshiro Tahara; Rie Wakabayashi; Noriho Kamiya; Masahiro Goto
  Membrane, THE MEMBRANE SOCIETY OF JAPAN, 38(2) 92 - 96, Mar. 2013
  

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• S/O-nanodispersion electrospun fiber mesh effective for sustained release of healthy plasmid DNA with the structural and functional integrity
  Tatsuya Okuda; Yoshiro Tahara; Noriho Kamiya; Masahiro Goto; Satoru Kidoaki
  JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, TAYLOR & FRANCIS LTD, 24(10) 1277 - 1290, Jan. 2013, Scientific journal
  

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• Transdermal delivery of insulin using a solid-in-oil nanodispersion enhanced by arginine-rich peptides
  Yoshiro Tahara; Shota Honda; Noriho Kamiya; Masahiro Goto
  MEDCHEMCOMM, ROYAL SOC CHEMISTRY, 3(12) 1496 - 1499, 22 Nov. 2012, Scientific journal
  

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• Solid-in-oil dispersion: A novel core technology for drug delivery systems
  Yoshiro Tahara; Noriho Kamiya; Masahiro Goto
  INTERNATIONAL JOURNAL OF PHARMACEUTICS, ELSEVIER SCIENCE BV, 438(1-2) 249 - 257, 15 Nov. 2012
  

  DOI URL
• A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells
  Yoshiro Tahara; Takeshi Kaneko; Riki Toita; Chiharu Yoshiyama; Takuya Kitaoka; Takuro Niidome; Yoshiki Katayama; Noriho Kamiya; Masahiro Goto
  JOURNAL OF CONTROLLED RELEASE, ELSEVIER SCIENCE BV, 161(3) 713 - 721, Aug. 2012, Scientific journal
  

  DOI URL
• Oil Gel Sheets Utilizing Solid–in–Oil Technique
  Asami Funatsu; Yoshiro Tahara; Sakurako Yamanaka; Masahiro Goto
  Membrane, THE MEMBRANE SOCIETY OF JAPAN, 36(2) 57 - 62, Mar. 2011
  

  DOI URL
• A Solid-in-Oil Dispersion of Gold Nanorods Can Enhance Transdermal Protein Delivery and Skin Vaccination
  Dakrong Pissuwan; Keisuke Nose; Ryohsuke Kurihara; Kenji Kaneko; Yoshiro Tahara; Noriho Kamiya; Masahiro Goto; Yoshiki Katayama; Takuro Niidome
  SMALL, WILEY-V C H VERLAG GMBH, 7(2) 215 - 220, Jan. 2011, Scientific journal
  

  DOI URL
• Transcutaneous immunization by a solid-in-oil nanodispersion
  Yoshiro Tahara; Kenichi Namatsu; Noriho Kamiya; Masayori Hagimori; Seitaro Kamiya; Masayuki Arakawa; Masahiro Goto
  CHEMICAL COMMUNICATIONS, ROYAL SOC CHEMISTRY, 46(48) 9200 - 9202, 28 Dec. 2010, Scientific journal
  

  DOI URL
• Ionic liquid-in-oil microemulsion as a potential carrier of sparingly soluble drug: Characterization and cytotoxicity evaluation
  Muhammad Moniruzzaman; Miki Tamura; Yoshiro Tahara; Noriho Kamiya; Masahiro Goto
  INTERNATIONAL JOURNAL OF PHARMACEUTICS, ELSEVIER SCIENCE BV, 400(1-2) 243 - 250, 15 Nov. 2010, Scientific journal
  

  DOI URL
• Ionic liquid-assisted transdermal delivery of sparingly soluble drugs
  Muhammad Moniruzzaman; Yoshiro Tahara; Miki Tamura; Noriho Kamiya; Masahiro Goto
  CHEMICAL COMMUNICATIONS, ROYAL SOC CHEMISTRY, 46(9) 1452 - 1454, 07 Mar. 2010, Scientific journal
  

  DOI URL
• A transdermal delivery system of an ascorbic acid derivative utilizing solid-in-oil technique
  Aiko Okuma; Hongyu Piao; Yoshiro Tahara; Noriho Kamiya; Masahiro Goto
  Membrane, THE MEMBRANE SOCIETY OF JAPAN, 34(4) 227 - 232, Jul. 2009
  

  DOI URL
• A solid-in-oil nanodispersion for transcutaneous protein delivery
  Yoshiro Tahara; Shota Honda; Noriho Kamiya; Hongyu Piao; Akihiko Hirata; Eiji Hayakawa; Takeru Fujii; Masahiro Goto
  JOURNAL OF CONTROLLED RELEASE, ELSEVIER SCIENCE BV, 131(1) 14 - 18, Oct. 2008, Scientific journal
  

  DOI URL
• Design and in vivo evaluation of solid-in-oil suspension for oral delivery of human growth hormone
  Hiromu Yoshiura; Yoshiro Tahara; Masakazu Hashida; Noriho Kamiya; Akihiko Hirata; Takeru Fujii; Masahiro Goto
  BIOCHEMICAL ENGINEERING JOURNAL, ELSEVIER SCIENCE SA, 41(2) 106 - 110, Sep. 2008, Scientific journal
  

  DOI URL

MISC

• ナノゲルとは？～あなたのナノ粒子はナノゲルといえるでしょうか？～
  田原義朗
  生物工学会誌, 102(1) 20 - 22, 25 Jan. 2024
• Self-Assembled Nanogels Consisting of Cholesterol-Bearing Polysaccharides and their Applications in Medicine
  Haddad Antonia-Nancy; Michiaki Matsumoto; Yoshiro Tahara
  Hydrogels and Nanogels - Applications in Medicine, Jun. 2023
  

  DOI URL
• イオン液体を利用した経皮吸収製剤の開発動向
  田原義朗; 後藤雅宏
  26 Aug. 2022
• 若手研究者からのメッセージ
  田原義朗
  日本化学会バイオテクノロジー部会NEWS LETTER, 26(1) 14 - 19, 01 Aug. 2022
• Solid-in-Oil Nanodispersion Technique for Transdermal Drug Delivery System of Biopharmaceutical Molecules
  TAHARA Yoshiro; GOTO Masahiro
  Oleoscience, Japan Oil Chemists' Society, 22(3) 121 - 126, 04 Mar. 2022
  

  DOI URL
• Current Advances of Transdermal Drug Delivery Systems Using Ionic Liquids
  Yoshiro Tahara; Masahiro Goto
  MEMBRANE, The Membrane Society of Japan, 46(5) 300 - 305, 05 Oct. 2021
  

  URLDOI URL
• Ionic Liquids for Transdermal Vaccination
  Yoshiro Tahara
  Application of Ionic Liquids in Drug Delivery, 10 187 - 193, 28 Aug. 2021
• Solid-in-oil-in-water(S/O/W)型多層エマルションとDDS への応用
  田原義朗; 後藤雅宏
  オレオサイエンス, 19(5) 15 - 20, Sep. 2019
• イオン液体という第３の液体を用いた創薬研究
  田原義朗; 後藤雅宏
  日本化学会バイオテクノロジー部会ニュースレター, 23(1) 3 - 12, Aug. 2019
• 油状ナノ基剤を用いたDDS 技術と非侵襲性経皮ワクチンへの応用
  田原義朗; 後藤雅宏
  化学工学, 83(7) 408 - 411, Jul. 2019
• イオン液体を用いた創薬研究
  田原義朗
  化学工学, 83(6) 365 , Jun. 2019
• S/O技術によるペプチドの経皮吸収
  Yoshiro Tahara; Masahiro Goto
  COSMETIC STAGE, 13(2) 48 - 52, Dec. 2018
• Solid-in-Oil(S/O)化技術と経皮ワクチンへの応用
  Yoshiro Tahara; Masahiro Goto
  PHARM TECH JAPAN, 34(14) 199 - 202, Nov. 2018
• イオン液体を用いたDDSと経皮デリバリーへの応用の現状
  Yoshiro Tahara; Masahiro Goto
  Drug Delivery System, 33(4) 303 - 310, Sep. 2018
• S/O 技術による皮膚浸透促進と化粧品開発
  Yoshiro Tahara; Masahiro Goto
  月刊ファインケミカル, 47(3) pp.38 - 44, Mar. 2018
• 微粒子等を用いたドラッグデリバリーシステム
  田原義朗; 秋吉一成
  動物用ワクチンとバイオ医薬品, pp.248 - 251, Jul. 2017
• 経皮吸収製剤における最新のＤＤＳ技術
  田原義朗; 後藤雅宏
  ＤＤＳ先端技術の先端バイオ医薬品への応用開発, 8(1) pp.404 - 409, Jun. 2017
• Transdermal protein delivery and immunization by a solid-in-oil nanodispersion technique
  Yoshiro Tahara; Masahiro Goto
  Drug Delivery System, Japan Society of Drug Delivery System, 32(3) 176 - 183, 2017, Book review
  

  DOI URL
• 自己組織化ナノゲルによるDDS開発
  田原義朗; 秋吉一成
  月刊ファインケミカル, シーエムシー出版, 45(11) pp.25 - 32, Nov. 2016
• 物理架橋ナノゲルの調製とDDS応用
  田原義朗; 秋吉一成
  DDSキャリア作製プロトコル集, pp.124 - 130, Aug. 2015
• Pullulan
  Yoshiro Tahara; Kazunari Akiyoshi
  Encyclopedia of polymeric nanomaterials, 2121 - 2129, Jun. 2015
• ナノゲル基盤材料によるタンパク質医薬の徐放技術とその応用
  田原義朗; 秋吉一成
  遺伝子医学MOOK別冊 – ここまで広がるドラッグ徐放技術の最前線 –, pp.124 - 130, Mar. 2013
• S/O®技術を用いたタンパク性医薬品の経皮吸収改善と経皮免疫への応用
  後藤雅宏; 田原義朗
  遺伝子医学MOOK別冊 – ペプチド・タンパク性医薬品の新規DDS製剤の開発と応用 –, pp.141 - 147, Jun. 2011
• タンパク質の経皮デリバリーは本当に可能か？
  後藤雅宏; 田原義朗
  化学, 化学同人, 66(5) pp.54 - 57, May 2011
• Development and clarification of a novel transcutaneous protein delivery system and its mechanism by a solid-in-oil nanodispersion
  Yoshiro Tahara; Shota Honda; Noriho Kamiya; Masahiro Goto
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, SOC BIOSCIENCE BIOENGINEERING JAPAN, 108 S39 - S40, Nov. 2009, Summary international conference
  

  DOI URL
• S/O化技術の魅力と新しい経皮薬物送達システム実現の可能性
  田原義朗; 神谷典穂; 後藤雅宏
  PHARM TECH JAPAN, 25 pp.1409 - 1414, Jun. 2009
• タンパク質の経皮デリバリーの実現
  田原義朗; 神谷典穂; 後藤雅宏
  バイオサイエンスとインダストリー, 67 pp.68 - 70, Apr. 2009

Works

• Peer-reviewed publications

Industrial Property Rights

• Patent right
  多孔質三次元細胞培養用足場材料及びその製造方法
  佐藤 良樹, 山本 健太, 堀口 智史, 田原 義朗, 山本 俊郎, 岸田 綱郎, 秋吉 一成, 松田 修
  JP2019033678, WO2020-045488, 京都府公立大学法人
• Patent right
  表面麻酔剤
  原田 耕志, タランヌム フェルドゥス, 後藤 雅宏, 田原 義朗
  特願2018-162980, 特開2020-033321, 国立大学法人山口大学, 国立大学法人九州大学
• Patent right
  免疫組成物
  後藤 雅宏, 田原 義朗, 水野 梨瑚
  特願2018-099129, 特開2019-202957, 国立大学法人九州大学
• Patent right
  Crosslinked hydrophobized-polysaccharide nanogel particles and manufacturing method therefor
  Kazunari Akiyoshi, Yoshiro Tahara, Sadaatsu Mukai, Shinichi Sawada
  JP2014059082, WO2014-157606, 国立大学法人京都大学
• Patent right
  Water-soluble drug carrier and process for production thereof
  Masahiro Goto, Noriho Kamiya, Yoshiro Tahara
  JP2010004021, WO2011-004552, 特許第5618307号, 国立大学法人九州大学

Research Projects

• がん治療時の口腔粘膜炎に対するアミノ酸併用新規支持療法の開発
  原田 耕志; 田原 義朗
  日本学術振興会, 科学研究費助成事業, 2023/04 -2026/03, 基盤研究(C), 山口大学
• Development of theranostics system for oral candidiasis
  Narisato Kanamura, Koichi Makimura, Ichiro Nishimura; Tetsuya Adachi; Yoshiro Tahara; Toshiro Yamamoto; Giuseppe Pezzotti
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), 2022/04 -2026/03, Coinvestigator, Grant-in-Aid for Scientific Research (C), Kyoto Prefectural University of Medicine
• Creation of Non-Invasive Transdermal Vaccine Using Solid-in-Oil Nano-dispersion Technique
  Masahiro Goto; Yoshiro Tahara; Noriho Kamiya; Rie Wakabayashi
  高効率ながん免疫、花粉症免疫の達成へ向け、まず令和3年度はS/O製剤の最適化および経皮免疫メカニズムの解明を行った。具体的には、(A) 抗原の高効率な皮膚浸透、(B) 抗原提示細胞への高効率デリバリー、(C) リンパ節中の免疫細胞の活性化の３つの過程に注目した。 本S/O製剤では、免疫系細胞の存在する角層下への浸透性のさらなる向上が、抗原提示細胞への高効率なデリバリーに繋がると期待した。そこで今回、膜透過性ペプチドであるオリゴアルギニンや、経皮吸収促進剤として疎水性イオン液体[Cho][FA] をS/O製剤に導入することで、抗原タンパク質の皮膚浸透性が向上し、抗体産生が有意に向上することを見出した。さらに、経皮ワクチンに最適な疎水性イオン液体の開発を通して、生体由来カチオンであるコリンを構成成分とした、皮膚浸透促進効果の高いイオン液体により、抗原の皮膚浸透と抗原提示細胞へのデリバリーの高効率化に成功した。 体内の免疫応答は、大きく液性免疫と細胞性免疫に分類され、互いにバランスを取り合っているが、がん免疫や花粉症免疫治療では、細胞性免疫の活性化が重要であることが知られている。そこで、細胞性免疫の活性化を達成するために、免疫活性化物質（アジュバント）の利用が有効であると考えた。特に、Toll様受容体（TLR）と呼ばれる、免疫細胞に発現している受容体が、免疫応答を制御していることが知られているため、TLR結合性リガンドをアジュバントとして用いた。本研究では特に、細胞性免疫を強力に誘導することが報告されている、CpG オリゴデオキシヌクレオチドやイミキモドの効果を確認した。 経皮がん免疫では、抗原として卵白由来アルブミン（OVA）を用い、腫瘍モデルとして、OVA恒常発現がん細胞株であるE.G7-OVAを移植した担がんマウス（C57BL/6N）の腫瘍成長抑制効果を明らかにした。, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), 2021/04 -2024/03, Coinvestigator, Grant-in-Aid for Scientific Research (A), Kyushu University
• Development of oil-based particles for oral drug delivery
  Yoshiro Tahara
  本研究では経口投与（飲み薬）として投与可能な油状微粒子の開発を行い、油状物質の吸収メカニズムの解明と、新しい経口デリバリーシステムの創出につながる研究を行うことを目的としている。初年度には油状微粒子として利用可能な物質の選定を行なっており、飲み薬として投与可能な成分のみによって構成されていること、薬を封入可能であること、粒子サイズをコントロールすることが可能であることに注目して検討を行った。その結果、油状物質はその融点が最も重要であることが分かり、界面活性剤にはそれ単独では薬の可溶化濃度が低い界面活性剤を利用する必要があることが分かった。またこのとき薬の封入特性についても定性的に検討を行った。その結果、油状物質を用いなかった場合には薬が沈殿する条件でも、ある油状物質を用いると薬が沈殿することなく水中に分散することが可能であることが明らかとなった。一方でこの油状物質で得られる油状微粒子は粒子サイズのコントロールは難しいことから、最終的な目標を達成するには、ある一つの油状物質を用いるのではなく、複数の油状物質を組合せることが必要であることが示唆される結果となった。今後は薬の封入性について定量的な検討を行い、最終的には同一の薬の投与量であるが、粒子サイズの異なる油状微粒子などを作成可能であるかを検討したい。またリパーゼなどを含む、消化管内の環境下においての薬の放出についても検討を行う予定である。, Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research, 2021/04 -2024/03, Principal investigator, Grant-in-Aid for Scientific Research (C), Doshisha University
• Creation of novel pharmaceutical application field utilizing ionic liquids
  後藤 雅宏; 田原 義朗; 原田 耕志; 若林 里衣
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Challenging Research (Pioneering), 2019/06 -2022/03, Challenging Research (Pioneering), Kyushu University
• Advanced Pharmaceutical Application of Ionic Liquids
  Masahiro Goto; Yoshiro Tahara; Koji Harada; Rie Wakabayashi
  In this study, biocompatible ionic liquids composed of biologically derived molecules were developed for pharmaceutical application. As biocompatible ionic liquids, ionic liquids composed of choline, amino acids, fatty acids, and phospholipids as materials were confirmed to be effective. The biocompatible ionic liquids also made it possible to construct transdermal vaccine formulations of cancer antigen peptides and transdermal peptide formulations such as insulin. The developed ionic liquids were also found to be low toxicity and low skin irritation formulations. The lipid-based ionic liquids achieved deep skin penetration of cancer antigens and efficient delivery to cutaneous dendritic cells, confirming their high antitumor efficacy., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Pioneering), 2020/04 -2022/03, Coinvestigator, Grant-in-Aid for Challenging Research (Pioneering), Kyushu University
• A solid-in-oil-in-water emulsion adjuvant for efficient vaccine delivery
  Tahara Yoshiro
  Vaccine is one of the most important strategy to prevent infectious diseases. In this study, a solid-in-oil-in-water (S/O/W) emulsion adjuvant was prepared. In the traditional emulsion adjuvant, antigens were added in the outer water phase and were separately delivered to antigen presenting cells. On the other hand, in the S/O/W emulsion adjuvant, antigens were encapsulated in the inner oil phase by solid-in-oil nanodispersion technology. From the vaccine study, the S/O/W emulsion produced the antigen-specific antibody and prevented tumor growth by antigen-specific immune responses compared to the traditional emulsion. This performance was resulted from the property of the S/O/W emulsion that deliver antigens to the antigen presenting cells together with adjuvant. These results suggested that the S/O/W emulsion is the promising vaccine delivery carrier., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A), 2017/04 -2021/03, Principal investigator, Competitive research funding, Grant-in-Aid for Young Scientists (A)
• Creation of Transdermal Drug Delivery Systems Using Solid-in-oil Nano-dispersion Technique
  Masahiro Goto; Yoshiro Tahara; Noriho Kamiya; Rie Wakabayashi; Kousuke Minamihata
  Non-invasive, painless transdermal delivery systems are attracting attention as an alternative drug administration method to conventional injections. In this study, we have developed a new transdermal delivery technology, called solid-in-oil (S/O) nano-dispersion technology, which enables simple and efficient transdermal delivery of biopharmaceutics. Using this S/O technology, peptides and proteins that have been difficult to deliver transdermally can now be delivered through the skin. Animal studies have confirmed that transdermal vaccines for cancer, hay fever, influenza, and malaria can be created by using this permeation technology, and that the vaccine effect (antibody production) is comparable to that of injections., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (S), 2016/05 -2021/03, Grant-in-Aid for Scientific Research (S), Kyushu University
• Creation of Transdermal Drug Delivery Systems Using Solid-in-oil Nano-dispersion Technique
  Goto Masahiro; KUBOTA Fukiko; KITAOKA Momoko; TAHARA Yoshiro
  Transdermal drug delivery is receiving a growing concern to alternate conventional drug administration techniques such as oral administration or needle-stick based administration methods. In this project, we proposed a patch system based on a solid-in-oil nanodispersion technique as a simple and efficient delivery method of drugs, especially protein and peptide vaccines, through the skin. The skin is an attractive route for vaccination, because there are many immune cells. We developed the solid-in-oil nanodisperison technique to deliver pharmaceutical bioactives efficiently through the skin. Solid-in-oil nanodispersions are nanosized drug carriers designed to overcome the skin barrier. Drug administration using a patch is user-friendly, and can improve patient compliance. Protein and peptide antigen drugs were efficiently delivered across the intact skin using the solid-in-oil nanodispersions. The technique is potent transcutaneous immunization method without needles., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (S), 2012/05 -2017/03, Grant-in-Aid for Scientific Research (S), Kyushu University
• Developmet of nanogel-cross-linked microspheres by bottom-up aproach and application for drug delivery system
  Tahara Yoshiro
  Bottom-up approach expanding attractive characters of nanometer-sized drug carriers into multifunctional drug delivery system is important for the next generation of biomaterials. Self-assembled nanogels have been known as one of the most beneficial nanometer-sized drug carriers. In this study, nanogel-cross-linked (NanoClik) microsphere was prepared through cross-linking of nanogels and demonstrated the application for drug delivery systems, in which micrometer-sized materials are needed. The most interesting characters of the NanoClik microspheres is that "drug-loaded nanogels" were released from the NanoClik microspheres following the hydrolysis of their cross-linker moieties. These system could be used to achieve the sustained delivery of drugs through the effective association of nanogels. These findings therefore suggest that NanoClik microspheres have high potential in terms of their application to the field of the nanocarrier-based material science., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), 2014/04 -2016/03, Principal investigator, Competitive research funding, Grant-in-Aid for Young Scientists (B), Kyoto University
• Raspberry-like nanogel-based material prepared by emulsion
  TAHARA Yoshiro
  Nanogel consisting of self-assembled polysaccharides is one of the most attractive carriers for drug delivery systems. In the present study, "raspberry-like nanogel-based material" was prepared by bottom-up approach using nanogels as building units. The effective control and design of the cross-linking and nano-domains in macro-scale hydrogels are important focuses of research. Raspberry-like nanogel-based material was prepared through cross-linking of nanogels in water-in-oil emulsion. The basic characters of raspberry-like nanogel-based materials were evaluated by confocal laser scanning microscopy and laser diffraction particle size analysis. The size of raspberry-like nanogel-based material was controlled in micrometer range. These findings suggested that raspberry-like nanogel-based material enables to be used as carrier for a novel drug delivery system., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up, 2012/08 -2014/03, Principal investigator, Competitive research funding, Grant-in-Aid for Research Activity Start-up, Kyoto University
• Transcutaneous vaccinations by a solid-in-oil nanodispersions
  Yoshiro Tahara
  過去の研究で申請者は独自の油状ナノ分散化技術を利用して、抗原を油状基剤中に分散させ、経皮浸透性が向上することを確認した。そこで当該年度では、本系を経皮免疫という実際のアプリケーションへ展開するための研究を行った。経皮免疫法とは抗原を皮膚から投与し、体内の抗原特異的な免疫力を増強・記憶させ、以後その病気にかかりにくくする塗り薬型のワクチン療法である。具体的には卵白アルブミンや卵白リゾチームをモデル抗原タンパク質として、これらを経皮吸収促進効果をもつ油状基剤へナノ分散したものを、マウスの耳介部、モルモットの背部、ウサギの耳介部などへ塗布(経皮デリバリー)し、血清中の抗体価の評価を行った。その結果、本手法によって全例について、血清中の抗体価が上昇し、免疫感受性の高いモルモットでは注射と同等の効果を得ることを確認した。また前年度までに申請者は、本技術を応用した新規細胞デリバリー用キャリアの開発を行ってきた。このキャリアは薬物(遺伝子やタンパク質)の周りを界面活性剤で2重に被覆した新しいダブルコーティングキャリアである。本キャリアは、内封する物質とキャリアの構成成分との間に特別な相互作用を必要とせず、遺伝子でもタンパク質でも封入可能であるという特徴をもつ。遺伝子を封入したキャリアについては、細胞毒性を示すこと無く遺伝子発現が可能であることを確認し、異なる等電点をもったカチオン性のタンパク質や、アニオン性のタンパク質であってもキャリアに封入可能かつ、細胞デリバリーが可能であるということを確認した。現在までにも遺伝子やタンパク質などからなる薬物を細胞デリバリー可能なキャリアは存在するが、その中でも本キャリアは、内封する薬物の種類が限定されないという優れた性質をもっていることから、経皮デリバリーのみに留まらず、大変多くの種類のドラッグデリバリーシステムへの応用が期待できる。, Japan Society for the Promotion of Science, Grant-in-Aid for JSPS Fellows, 2009/04 -2011, Principal investigator, Competitive research funding, Grant-in-Aid for JSPS Fellows, Kyushu University