| TSUKAGOSHI Kazuhiko |  |
| Faculty of Science and Engineering Department of Chemical Engineering and Materials Science | |
| Professor |
Last Updated :2025/05/23
Researcher Profile and Settings
researchmap
Research Areas
Research Experience
- Doshisha University, Faculty of Science and Engineering, professor, 2001/04 - Today
- Doshisha University, 工学部, associate professor, 1995/04 - 2000/03
- Kyushu University, School of Engineering, Assistant Professor, 1993/09 - 1995/03
- Indiana University, postdoctral researcher, 1994/01 - 1995/01
- Kyushu University, School of Engineering, Research Associate, 1990/04 - 1993/08
- 同志社大学大学院工学研究科博士課程（後期）, 1987/04 - 1990/03
- 富士ゼロックス株式会社（総合研究所）, 1984/04 - 1986/05
- 同志社大学大学院工学研究科博士課程（前期）, 1982/04 - 1984/03
Education
- 同志社大学大学院, 工学研究科, 工業化学専攻 博士課程後期, 1987 - 1990
- Doshisha University, 工学研究科, 工業化学専攻 博士課程前期, 1982 - 1984
- Doshisha University, Faculty of Engineering, 化学工学科, 1978 - 1982
Degree
Association Memberships
Committee Memberships
- 編集委員, 2017 - Today, Journal of flow injection analysis
- 委員, 2012 - Today, 日本分析化学会 フローインジェクション分析研究会
- 電気泳動分析研究懇談会委員, 2010 - Today, 日本分析化学会
- 評議員, 2003 - Today, 大学等環境安全協議会
- 近畿支部幹事（常任幹事期間を除く）, 2001 - Today, 日本分析化学会
- 評議員, 2018 - 2019, 関西工学教育協会
- 特別研究委員事業等審査委員・評価員, 2018 - 2019, 日本学術振興会
- 近畿支部常任幹事, 2018 - 2019, 日本分析化学会
- 委員, 2017 - 2019, 近畿化学協会
- 第53回フローインジェクション分析講演会実行委員長, 2016 - 2016, フローインジェクション分析研究会
- 科学研究費助成事業審査委員, 2012 - 2013, 日本学術振興会
- 技術評価委員, 2007 - 2012, 大阪投資育成第５投資事業有限責任組合
- 「ぶんせき」（月刊誌）編集委員, 2009 - 2010, 日本分析化学会
- 近畿支部常任幹事, 2009 - 2010, 日本分析化学会
- 編集委員, 2005 - 2006, Analytical Sciences
- 近畿支部常任幹事, 2002 - 2003, 日本化学会
- 近畿支部常任幹事, 2002 - 2003, 日本分析化学会
- 近畿支部幹事, 2001 - 2003, 日本化学会
- 九州支部庶務幹事, 1993 - 1993, 日本分析化学会
Awards
- Hot Article Award Analytical Sciences (September 15, 2023)
2023 - Hot Article Award Analytical Sciences (April 15, 2023)
2023 - Hot Article Award Analytical Sciences (July 15, 2022)
2022 - FIA Award for Original Papers (2019)
2019 - Flow-injection Analysis Scientific Award
2015 - Most Cited Paper Award of Analytical Sciences 2012
2013 - Hot Article Award Analytical Sciences (May 10, 2012)
2012, Japan - Hot Article Award Analytical Sciences (November 10, 2008)
2008, Japan - Top Cited Article Award, Journal of Chromatography (Elsevier; 2000-2004)
2005 - The Japan Society for Analytical Chemistry Incentive Award
1995, Japan
Published Papers
- Development of an HPLC System Using a Phase-Separation Multiphase Flow as an Eluent— Effect of Pore Size in Column-Packed Particles on the Novel Separation Mode —
Daiki ISHIKAWA; Yuki OBATA; Hiroshi SAKAMAKI; Takeshi IHARADA; Kazuhiko TSUKAGOSHI
BUNSEKI KAGAKU, Japan Society for Analytical Chemistry, 73(9) 505 - 514, 05 Sep. 2024, Scientific journal - Development of HPLC Device with Water/Acetonitrile with NaCl Mixed Solution That Induces a Phase-Separation Multiphase Flow as the Eluent in the Separation Column
Ryuto Hirose; Takeshi Iharada; Kazuhiko Tsukagoshi
Analytical Sciences, 40 1779 - 1785, Aug. 2024, Scientific journal - Development of a HPLC system using a phase-separation multiphase flow as an eluent: an influence of column pressure on phase separation and chromatogram at room temperature
Keisuke Takagi; Takeshi Iharada; Kazuhiko Tsukagoshi
Analytical Sciences, Springer Science and Business Media LLC, 40(11) 2081 - 2087, 30 Jul. 2024, Scientific journal - Evaluation of Reactive Oxygen Species (ROS) Generated on the Surface of Copper Using Chemiluminesence
Ken Hirota; Hiroya Tanaka; Taika Maeda; Kazuhiko Tsukagoshi; Hiroshi Kawakami; Takashi Ozawa; Masahiko Wada
Materials Sciences and Applications, 14 482 - 499, 2023 - Development of a HPLC System Using a Phase-Separation Multiphase Flow as an Eluent Coupled to a Silica-Particle Packed Column
Satoru Kinoshita; Daiki Ishikawa; Yuki Kobayashi; Yuki Obata; Hiroshi Sakamaki; Takeshi Iharada; Kazuhiko Tsukagoshi
Analytical Sciences, 39 1483 - 1491, 2023 - Development of HPLC System That Uses Phase-Separation Multiphase Flow as an Eluent
Noé Jallas; Daiki Ishikawa; Tetsuro Katayama; Aoi Kimura; Satoru Kinoshita; d Kazuhiko
Analytical Sciences, 39 883 - 892, 2023, Scientific journal - Antibacterial Activity of Anatase TiO2 Added Cu Powder
Ken Hirota; Ayumu Jinzenji; Kazuhiko Tsukagoshi; Yurika Taniguchi; Hiroshi Kawakami; Takashi Ozawa; Masahiko Wada; Yoji Yuki
Powder Powder Metallurgy, 70 121 - 131, 2023, Scientific journal - Observation of the Phase-Separation Multiphase Flow Using a Polyethylene Glycol/Phosphate Mixed Solutions and the Aqueous Two-Phase Distribution of Red Blood Cells in the Flow System
Kazushi Nishimura; Chihiro Matsushita; Kenichi Yamashita; Masaharu Murata; Kazuhiko Tsukagoshi
Analytical Sciences, 39(4) 537 - 546, 2023, Scientific journal - Microfluidic behavior of ternary mixed solutions of water/acetonitrile/ethyl acetate through experiments and computer simulations
Keigo Yonekura; Kazushi Nishimura; Katsumi Tsuchiya; Kenichi Yamashita; Masaharu Murata; Kazuhiko Tsukagoshi
Analytical Sciences, Springer Science and Business Media LLC, 38(4) 731 - 736, Apr. 2022, Scientific journal - Consecutive Sample Injection analysis in Tube Radial Distribution Chromatography
Yusaku Takahashi; Kazushi Nishimura; Kazuhiko Tsukagoshi; Katsumi Tsuchiya; Ken Hirota; Kenichi Yamashita; Masaharu Murata
Analytical Sciences, 37(10) 1373 - 1377, 2021, Scientific journal - Separation of Dansyl-DL-Amino Acids Through Tube Radial Distribution Chromatography by Using a Commercially Available HPLC System with a Capillary Tube Manufactured for GC as a Separation Column
Nobuyuki Sanada; Kazuhiko Tsukagoshi; Katsumi Tsuchiya; Ken Hirota; Kenichi Yamasita; Masaharu Murata
Chromatography, 42 67 - 71, 2021 - Preparation of Anatase Titanium Dioxide NanoparticlePowders Submitting Reactive Oxygen Species (ROS) under Dark Conditions
Thi Minh Phuong Nguyen; Perrine Lemaitre; Masaki Kato; Ken Hirota; Kazuhiko Tsukagoshi; Hirohisa Yamada; Atsuki Terabe; Hideto Mizutani; Shingo Kanehira
Materials Sciences and Applications, 12 89 - 110, 2021 - Control of Phase Configuration of an Annular Flow in a Capillary Tube through a Mixing Joint
Ryota Shiraishi; Kazuhiko Tsukagoshi; Katsumi Tsuchiya; Ken Hirota; Kenichi Yamashita; Masaharu Murata
Journal of Flow Injection Analysis, 37 13 - 18, Apr. 2020 - Investigation of the Separation Efficiency of Tube Radial Distribution Chromatography with Stationary Outer Phase Using the van Deemter Equation
Chihiro Matsushita; Kazuhiko Tsukagoshi; Katsumi Tsuchiya; Kenichi Yamashita; Masaharu Murata
Chromatographia, SPRINGER HEIDELBERG, 83(2) 287 - 292, 01 Feb. 2020, Scientific journal - Development of α-Amino Acid Detection with Peroxyoxalate Chemiluminescence by Using Water-Acetonitrile-Ethyl Acetate Mixed Solution
Hyo Kan; Ryo Mizutani; Kazuhiko Tsukagoshi
American Journal of Analytical Chemistry, 11 15 - 24, 2020 - The Study of Physicochemical Properties of Antibacterial ZnO Powder : Impurity Doping, Elution and Catalytic Properties
Nguyen Phuong Thi Minh; Tokuoka Yuki; Imai Wakana; Hirota Ken; Kato Masaki; Tsukagoshi Kazuhiko
同志社大学ハリス理化学研究報告 = The Harris science review of Doshisha University, Alternative:Harris Science Research Institute of Doshisha University, 60(2) 105 - 113, Jul. 2019 - Mixing Process of Ternary Mixed Solvent Solutions in a Tapered-Microchannel and Application to Analysis Method of Mixing Characteristics in Microfluidic Channel (JAFIA創立35周年記念号)
Mori Ryuki; Nakata Yuko; Tsukagoshi Kazuhiko; Tsuchiya Katsumi; Hirota Ken; Yamashita Kenichi; Murata Masaharu
Journal of flow injection analysis, 日本分析化学会フローインジェクション分析研究懇談会, 36(1) 19 - 25, Jun. 2019 - 相分離混相流の発見とクロマト分離技術への応用 (特集 クロマト分離技術の最前線)
塚越 一彦
分離技術, 分離技術会, 49(1) 24 - 28, 2019 - Confirmation of Separation Mechanism Through Visualization of Microfluidic Behavior of Fluorescent Analytes in Tube Radial Distribution Chromatography
TAKATSUKI Yosuke; TSUKAGOSHI Kazuhiko; TSUCHIYA Katsumi; YAMASHITA Kenichi; MURATA Masaharu
CHROMATOGRAPHY, The Society for Chromatographic Sciences, 40(3) 163 - 168, 2019 - Dependence of antibacterial activity of ZnO powders on their physico-chemical properties
Thi Minh Phuong Nguyen; Ken Hirota; Masaki Kato; Kazuhiko Tsukagoshi; Hirohisa Yamada; Atsuki Terabe; Hideto Mizutani
Funtai Oyobi Fummatsu Yakin/Journal of the Japan Society of Powder and Powder Metallurgy, Japan Society of Powder and Powder Metallurgy, 66(9) 434 - 441, 2019, Scientific journal - Microfluidic inverted flow of ternary water/hydrophilic/ hydrophobic organic solvent solution in a Y-type microchannel and a proposal of the response microfluidic analysis through the experiment
Bun Yamawaki; Ryuki Mori; Kazuhiko Tsukagoshi; Katsumi Tsuchiya; Kenichi Yamashita; Masaharu Murata
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 35(3) 249 - 256, 2019, Scientific journal - Development of tube radial distribution chromatography based on phase-separation multiphase flow created via pressure loss
Susumu Wada; Kazuhiko Tsukagoshi; Katsumi Tsuchiya; Ken Hirota; Kenichi Yamashita; Masaharu Murata
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 35(7) 803 - 806, 2019, Scientific journal - Phase separation and collection of annular flow by phase transformation
Aya Yoshioka; Kazuhiko Tsukagoshi; Katsumi Tsuchiya; Ken Hirota; Kenichi Yamashita; Masaharu Murata
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 35(11) 1279 - 1282, 2019, Scientific journal - Tube radial distribution chromatography system developed by combining commercially available HPLC system and open-tubular capillary tube as separation column
Kento Yamada; Hyo Kan; Kazuhiko Tsukagoshi
Talanta, ELSEVIER SCIENCE BV, 183 89 - 93, 01 Jun. 2018, Scientific journal - Preparation of ZnO powders with strong antibacterial activity under dark conditions
Thi Minh Phuong Nguyen; Shoko Hirota; Yusei Suzuki; Masaki Kato; Ken Hirota; Hideki Taguchi; Hirohisa Yamada; Kazuhiko Tsukagoshi
Funtai Oyobi Fummatsu Yakin/Journal of the Japan Society of Powder and Powder Metallurgy, Japan Society of Powder and Powder Metallurgy, 65(6) 316 - 324, 2018, Scientific journal - Implementation of tube radial distribution chromatography by using a commercially available HPLC system
Hyo Kan; Kento Yamada; Nobuyuki Sanada; Koyo Nakata; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 34(2) 239 - 241, 2018, Scientific journal - Phase separation multi-phase flow using an aqueous two-phase system of a polyethylene glycol/dextran mixed solution
Naoya Imanishi; Tetsuo Yamasaki; Kazuhiko Tsukagoshi; Masaharu Murata
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 34(8) 953 - 958, 2018, Scientific journal - Protein separation through preliminary experiments concerning pH and salt concentration by tube radial distribution chromatography based on phase separation multiphase flow using a polytetrafluoroethylene capillary tube
Hyo Kan; Kazuhiko Tsukagoshi
Talanta, ELSEVIER SCIENCE BV, 169 130 - 135, 01 Jul. 2017, Scientific journal - A poly(dimethylsiloxane) microfluidic sheet reversibly adhered on a glass plate for creation of emulsion droplets for droplet digital PCR
Yuta Nakashoji; Hironari Tanaka; Kazuhiko Tsukagoshi; Masahiko Hashimoto
Electrophoresis, WILEY-BLACKWELL, 38(2) 296 - 304, 01 Jan. 2017, Scientific journal - The 53rd Annual Meeting of Japan Association for Flow Injection Analysis:Report
塚越 一彦
Journal of Flow Injection Analysis, Japanese Association for Flow Injection Analysis, 33(2) 119 - 119, 2016 - Improvement of the mutation-discrimination threshold for rare point mutations by a separation-free ligase detection reaction assay based on fluorescence resonance energy transfer
Kenta Hagihara; Kazuhiko Tsukagoshi; Chinami Nakajima; Shinsuke Esaki; Masahiko Hashimoto
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 32(3) 367 - 370, 2016, Scientific journal - Consideration of inner and outer phase configuration in tube radial distribution phenomenon based on viscous dissipation in a microfluidic flow using various types of mixed solvent solutions
Satoshi Fujinaga; Masahiko Hashimoto; Kazuhiko Tsukagoshi; Jiro Mizushima
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 32(4) 455 - 461, 2016, Scientific journal - Tube radial distribution flow separation in a microchannel using an ionic liquid aqueous two-phase system based on phase separation multi-phase flow
Kosuke Nagatani; Yoshinori Shihata; Takahiro Matsushita; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 32(12) 1371 - 1374, 2016, Scientific journal - Microflow extraction using a microchip incorporating microchannels based on the tube radial distribution phenomenon
Naomichi Suzuki; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Solvent Extraction Research and Development, JAPAN ASSOC SOLVENT EXTRACTION, 23(1) 115 - 120, 2016, Scientific journal - Consideration of tube radial distribution phenomenon under laminar flow conditions based on the weber number
Satoshi Fujinaga; Masahiko Hashimoto; Kazuhiko Tsukagoshi; Jiro Mizushima
Journal of Chemical Engineering of Japan, SOC CHEMICAL ENG JAPAN, 48(12) 947 - 952, 20 Dec. 2015, Scientific journal - Microfluidic Inverted Flow of Aqueous and Organic Solvent Mixed Solution in a Microchannel under Laminar Flow Conditions
MuraMurakami Shunpei; Fujinaga Satoshi; Hashimoto Masahiko; Tsukagoshi Kazuhiko
The Harris science review of Doshisha University, Alternative:Harris Science Research Institute of Doshisha University, 56(3) 155 - 159, Oct. 2015 - Hands-off preparation of monodisperse emulsion droplets using a poly(dimethylsiloxane) microfluidic chip for droplet digital PCR
Hironari Tanaka; Shunsuke Yamamoto; Arichika Nakamura; Yuta Nakashoji; Naoaki Okura; Norimitsu Nakamoto; Kazuhiko Tsukagoshi; Masahiko Hashimoto
Analytical Chemistry, AMER CHEMICAL SOC, 87(8) 4134 - 4143, Apr. 2015, Scientific journal - Investigation of the composition for a ternary solvent system in tube radial distribution chromatography
Satoshi Fujinaga; Masahiko Hashimito; Kazuhiko Tsukagoshi
Journal of Liquid Chromatography and Related Technologies, TAYLOR & FRANCIS INC, 38(5) 600 - 606, 16 Mar. 2015, Scientific journal - Open-tubular capillary chromatoraphy based on tube radial distribution of the water-acetonitrile containing sodium chloride mixture carrier solvents
Tomoya Kobayashi; Hyo Kan; Kisuke Tabata; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Journal of Liquid Chromatography and Related Technologies, TAYLOR & FRANCIS INC, 38(1) 44 - 53, 02 Jan. 2015, Scientific journal - Discover and investigation of a phase-separated solvent mixture as a carrier solution and its applications for microflow chemical techniques:FIA Award for Science
Tsukagoshi Kazuhiko
Journal of Flow Injection Analysis, Japanese Association for Flow Injection Analysis, 32(2) 66 - 66, 2015 - Development of Capillary Chromatography Based on Specific Fluidic Behavior of the Solvents in a Microspace
韓 氷; 橋本 雅彦; 塚越 一彦
同志社大学理工学研究報告, 同志社大学理工学研究所, 55(4) 9 - 11, Jan. 2015 - Investigation of Tube Radial Distribution Phenomenon and Its Functional Appearance
塚越 一彦
同志社大学理工学研究報告, 同志社大学理工学研究所, 55(4) 76 - 78, Jan. 2015 - Investigation of Specific Microfluidic Flow with Two-phase Separation Mixed Solvent Solutions and Application to Flow Technology
Tsukagoshi Kazuhiko
Journal of Flow Injection Analysis, Japanese Association for Flow Injection Analysis, 32(2) 89 - 89, 2015 - Tube radial distribution chromatography on a microchip incorporating microchannels with a three-to-one channel confluence point
Naomichi Suzuki; Kenichi Yamashita; Hideaki Maeda; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 31(12) 1267 - 1272, 2015, Scientific journal - A microbead-based single base extension assay for the detection of known single-base changes in genomic DNA
Kohei Fujishima; Shunsuke Yamamoto; Kazuhiko Tsukagoshi; Masahiko Hashimoto
Chemistry Letters, CHEMICAL SOC JAPAN, 44(5) 595 - 597, 2015, Scientific journal - Separation of metal complexes with counter ions by tube radial distribution chromatography using a ternary solvent containing 8-quinolinol
Yuji Kawai; Satoshi Fujinaga; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 31(11) 1177 - 1182, 2015, Scientific journal - A microflow-extraction system using double tubes having different inner diameters in tube radial distribution phenomenon
Katsuya Unesaki; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Solvent Extraction Research and Development, JAPAN ASSOC SOLVENT EXTRACTION, 22(1) 87 - 93, 2015, Scientific journal - Microchip Capillary Chromatography with Chemiluminescence Detection Based on Tube Radial Distribution Phenomenon
Takafumi Matsuda; Masahiko Hashimoto; Kazuhiko Tsukagoshi
LUMINESCENCE, WILEY-BLACKWELL, 29 49 - 50, Aug. 2014 - Discriminative detection of low-abundance point mutations using a PCR/ligase detection reaction/capillary gel electrophoresis method and fluorescence dual-channel monitoring
Mariko Hamada; Koji Shimase; Kazuhiko Tsukagoshi; Masahiko Hashimoto
Electrophoresis, WILEY-BLACKWELL, 35(8) 1204 - 1210, Apr. 2014, Scientific journal - Microbead-based ligase detection reaction assay using a molecular beacon probe for the detection of low-abundance point mutations
Sho Watanabe; Kenta Hagihara; Kazuhiko Tsukagoshi; Masahiko Hashimoto
Analytical Chemistry, AMER CHEMICAL SOC, 86(1) 900 - 906, 07 Jan. 2014, Scientific journal - Investigation of inner and outer phase formation in tube radial distribution phenomenon using various types of mixed solvent solutions
Satoshi Fujinaga; Katsuya Unesaki; Yuki Kawai; Koichi Kitaguchi; Kosuke Nagatani; Masahiko Hashimoto; Kazuhiko Tsukagoshi; Jiro Mizushima
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 30(10) 1005 - 1011, 2014, Scientific journal - Tube radial distribution phenomenon with a two-phase separation solution of a fluorocarbon and hydrocarbon organic solvent mixture in a capillary tube and metal compounds separation
Koichi Kitaguchi; Naoya Hanamura; Masaharu Murata; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 30(6) 687 - 690, 2014, Scientific journal - Capillary chromatography using an annular and sluggish flow in the ternary water-acetonitrile-ethyl acetate system as carrier solution
Yuya Hamaguchi; Satoshi Fujinaga; Shunpei Murakami; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Chemistry Letters, CHEMICAL SOC JAPAN, 43(8) 1318 - 1320, 2014, Scientific journal - Fundamental research and application of the specific fluidic behavior of mixed solvents in a microspace
Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 30(1) 65 - 73, Jan. 2014, Scientific journal - Tube radial distribution phenomenon observed in an aqueous micellar solution of non-ionic surfactant fed into a microspace and an attempt of capillary chromatographic application
N. Jinno; K. Unesaki; M. Hashimoto; K. Tsukagoshi
Journal of Analytical Chemistry, 68(12) 1070 - 1075, Dec. 2013, Scientific journal - Development of a ligase detection reaction/CGE method using a LIF dual-channel detection system for direct identification of allelic composition of mutated DNA in a mixed population of excess wild-type DNA
Mariko Hamada; Koji Shimase; Keiichi Noda; Kazuhiko Tsukagoshi; Masahiko Hashimoto
Electrophoresis, WILEY-BLACKWELL, 34(9-10) 1415 - 1422, May 2013, Scientific journal - Michrochip chromatography using an open-tubular microchannel and a ternary water-ACN-ethyl acetate mixture carrier solution
Takafumi Matsuda; Kenichi Yamashita; Hideaki Maeda; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Journal of Separation Science, WILEY-V C H VERLAG GMBH, 36(5) 965 - 970, Mar. 2013, Scientific journal - Capillary Chromatography Based on Tube Radial Distribution of Ternary Mixed Solvents : Construction of the Phase Diagram and the Separation Performance
Tanigawa Yusuke; Fujinaga Satoshi; Hashimoto Masahiko; Tsukagoshi Kazuhiko
The science and engineering review of Doshisha University, Alternative:Science and Engineering Research Institute of Doshisha University, 53(4) 167 - 172, Jan. 2013 - Tube Radial Distribution Phenomenon and Its Applications : Microreactor by Taking Advantage of Kinetic Liquid-Liquid Interface as a Chemical Reaction Space (第50回同志社大学理工学研究所研究発表会 2012年度学内研究センター合同シンポジウム 講演予稿集)
TSUKAGOSHI Kazuhiko
同志社大学理工学研究報告, 同志社大学理工学研究所, 53(4) 50 - 52, Jan. 2013 - Development of a PCR/LDR/Flow-Through hybridization assay using a capillary tube, probe dna-immobilized magnetic beads and chemiluminescence detection
Manami Hommatsu; Hisamitsu Okahashi; Keisuke Ohta; Yusuke Tamai; Kazuhiko Tsukagoshi; Masahiko Hashimoto
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 29(7) 689 - 695, 2013, Scientific journal - Specific distribution behavior of a ternary mixture of solvents fed into bent and wound microchannels in microchips
Kei Nishiyama; Masaharu Murata; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 29(10) 1003 - 1008, 2013, Scientific journal - Two-phase extraction of metal ions using a water-acetonitrile-ethyl acetate ternary mixed-solvent separation system
Naoya Takahashi; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 29(6) 665 - 667, 2013, Scientific journal - Examination of tube radial distribution phenomenon and its function appearance
Kazuhiko Tsukagoshi
Bunseki Kagaku, JAPAN SOC ANALYTICAL CHEMISTRY, 62(5) 393 - 407, 2013, Scientific journal - Specific microfluidic behavior of ternary mixed carrier solvents of water-acetonitrile-ethyl acetate in open-tubular capillary chromatography and the chromatograms
Satoshi Fujinaga; Katsuya Unesaki; Shigeru Negi; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Methods, ROYAL SOC CHEMISTRY, 4(11) 3884 - 3890, Nov. 2012, Scientific journal - Tube Radial Distribution Phenomenon and Its Application
藤永 慧; 塚越 一彦
化学とマイクロ・ナノシステム : 化学とマイクロ・ナノシステム研究会誌, 化学とマイクロ・ナノシステム研究会, 11(2) 10 - 15, Oct. 2012 - Study of outer phases in capillary chromatography based on tube radial distribution of carrier solvents under laminar flow conditions
Naoya Jinno; Mari Murakami; Kiyoshi Mizohata; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Journal of Liquid Chromatography and Related Technologies, TAYLOR & FRANCIS INC, 35(12) 1750 - 1766, 15 Jul. 2012, Scientific journal - Single Point Mutation Analysis Using Magnetic Beads Coated with Streptavidin
Hashimoto Masahiko; Fujita Taku; Tsukagoshi Kazuhiko
The science and engineering review of Doshisha University, Alternative:Science and Engineering Research Institute of Doshisha University, 53(2) 59 - 66, Jul. 2012 - Capillary electrophoresis with a chemiluminescence detector using the two reactions of luminol and peroxyoxalate
Kazumasa Tsuge; Takayuki Tanaka; Keiichi Noda; Kazuhiko Tsukagoshi
Journal of Liquid Chromatography and Related Technologies, TAYLOR & FRANCIS INC, 35(8) 1091 - 1101, 01 May 2012, Scientific journal - Separation of dansyl-dl-amino acids by open tubular capillary chromatography based on tube radial distribution phenomenon of the ternary mixed carrier solvents
Yudai Kudo; Hyo Kan; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Methods, ROYAL SOC CHEMISTRY, 4(4) 906 - 912, Apr. 2012, Scientific journal - Tentative comparison of tube radial distribution chromatography and CZE
Kisuke Tabata; Naoya Jinno; Keiichi Noda; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Chromatographia, SPRINGER HEIDELBERG, 75(7-8) 423 - 428, Apr. 2012, Scientific journal - Effects of tube materials on capillary chromatography based on tube radial distribution of ternary mixture carrier solvents under laminar flow conditions
Yudai Kudo; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Chromatographia, SPRINGER HEIDELBERG, 75(7-8) 417 - 421, Apr. 2012, Scientific journal - Fluidic Behavior of Polymer Compounds in an Open-Tubular Capillary with Ternary Mixed Carrier Solvents under Laminar Flow Conditions:Original Paper
Nogami Takahiro; Fujinaga Satoshi; Hashimoto Masahiko; Tsukagoshi Kazuhiko
Journal of Flow Injection Analysis, Japanese Association for Flow Injection Analysis, 29(1) 21 - 21, 2012 - Development of Solvent Extraction Method Based on Tube Radial Distribution Phenomenon
神野 直哉; 橋本 雅彦; 塚越 一彦
同志社大学理工学研究報告, 同志社大学理工学研究所, 52(4) 35 - 37, Jan. 2012 - Consideration of the tube radial distribution of the carrier solvents in a capillary tube under laminar flow conditions and computer simulation
Naoya Jinno; Yuji Masuhara; Tomoya Kobayashi; Naoya Tainaka; Yusuke Tanigawa; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 28(5) 527 - 530, 2012, Scientific journal - Biomolecule analyses in an open-tubular capillary chromatography using ternary mixed carrier solvents with chemiluminescence detection
Naoya Takahashi; Yuji Masuhara; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 28(4) 351 - 358, 2012, Scientific journal - Elution behavior of Lambda-DNA with ternary mixed carrier solvents in an open-tubular capillary under laminar flow conditions
Takahiro Nogami; Satoshi Fujinaga; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 28(6) 617 - 620, 2012, Scientific journal - Mixing process of ternary solvents prepared through microchannels in a microchip under laminar flow conditions
Kei Nishiyama; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 28(4) 423 - 428, 2012, Scientific journal - The micro-flow reaction system featured the liquid-liquid interface created with ternary mixed carrier solvents in a capillary tube
Yuji Masuhara; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 28(5) 439 - 444, 2012, Scientific journal - Investigations into tie lines and solubility curves on phase diagrams in Open-Tubular capillary chromatography using ternary Mixed-Carrier Solvents
Yusuke Tanigawa; Satoshi Fujinaga; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 28(9) 921 - 924, 2012, Scientific journal - Tube radial distribution of solvents observed in an aqueous ionic liquid: Mixed solution delivered into a capillary tube
Yuki Kawai; Masaharu Murata; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 28(11) 1029 - 1031, 2012, Scientific journal - Rapid and convenient sample preparation in a single tube using magnetic beads for fluorescence detection of single nucleotide variation based on oligonucleotide ligation
Masahiko Hashimoto; Chika Morimoto; Kenta Hagihara; Kazuhiko Tsukagoshi
Chemistry Letters, CHEMICAL SOC JAPAN, 41(2) 135 - 137, 2012, Scientific journal - Influence of adding surfactants to an analyte solution on separation performance in open-tubular capillary chromatography based on the tube radial distribution of ternary mixed carrier solvents
Katsuya Unesaki; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Chemistry Letters, CHEMICAL SOC JAPAN, 41(9) 855 - 856, 2012, Scientific journal - Chromatography using ternary water-acetonitrile-ethyl acetate mixture as a carrier solution on a microchip incorporating microchannels
Takafumi Matsuda; Naoya Jinno; Kenichi Yamashita; Hideaki Maeda; Akihiro Arai; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Chemistry Letters, CHEMICAL SOC JAPAN, 41(11) 1448 - 1450, 2012, Scientific journal - Components of the Carrier Solvents and Separation Performance in the Tube Radial Distribution Chromatography Using a Fused-Silica Capillary Tube
Jinno Naoya; Hayashi Yoichiro; Hashimoto Masahiko; Tsukagoshi Kazuhiko
The science and engineering review of Doshisha University, Alternative:Science and Engineering Research Institute of Doshisha University, 52(3) 177 - 180, Oct. 2011 - Use of tube radial distribution of ternary mixed carrier solvents for introduction of absorption reagent for metal ion separation and online detection into capillary
Satoshi Fujinaga; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Journal of Separation Science, WILEY-V C H VERLAG GMBH, 34(20) 2833 - 2839, Oct. 2011, Scientific journal - Development of DNA point mutation detection methods using probe DNA-immobilized microbeads
Hashimoto Masahiko; Tamai Yusuke; Tsukagoshi Kazuhiko
The Science and engineering review of Doshisha University, Alternative:Science and Engineering Research Institute of Doshisha University, 52(2) 93 - 98, Jul. 2011 - Extraction of Cu(II) Based on Tube Radial Distribution of Ternary Mixed Carrier Solvent in Microchannels
Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
CHEMISTRY LETTERS, CHEMICAL SOC JAPAN, 40(6) 654 - 655, Jun. 2011, Scientific journal - Fluorescence observation supporting capillary chromatography based on tube radial distribution of carrier solvents under laminar flow conditions
Naoya Jinno; Mari Murakami; Kiyoshi Mizohata; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analyst, ROYAL SOC CHEMISTRY, 136(5) 927 - 932, 07 Mar. 2011, Scientific journal - 月例卓話 微小空間内の特異的な溶媒分配挙動に基づくキャピラリークロマトグラフィーの開発
藤永 慧; 塚越 一彦
海洋化学研究, 海洋化学研究所, 24(2) 81 - 85, 2011 - Metal compound analysis by capillary chromatography using an untreated capillary tube and water-hydrophilic-hydrophobic solvent mixture as a carrier solution
Yoshida Kazuma; Jinno Naoya; Hashimoto Masahiko; Tsukagoshi Kazuhiko
The Science and engineering review of Doshisha University, Alternative:Science and Engineering Research Institute of Doshisha University, 51(4) 198 - 201, Jan. 2011 - Influences of Analyte Injection Volumes and Concentrations on Capillary Chromatography Based on Tube Radial Distribution of Carrier Solvents under Laminar Flow Conditions
Tanigawa Yusuke; Jinno Naoya; Hashimoto Masahiko; Tsukagoshi Kazuhiko
CHROMATOGRAPHY, The Society for Chromatographic Sciences, 32(3) 135 - 140, 2011 - Instrumentation of a plc-regulated temperature cycler with a PID control unit and its use for miniaturized PCR systems with reduced volumes of aqueous sample droplets isolated in oil phase in a microwell
Masahiko Hashimoto; Masayuki Torii; Kazuma Yoshida; Keiichi Noda; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 27(12) 1191 - 1196, 2011, Scientific journal - Tube radial distribution phenomenon of ternary mixed solvents in a microspace under laminar flow conditions
Mari Murakami; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 27(8) 793 - 798, 2011, Scientific journal - Experimental consideration of capillary chromatography based on tube radial distribution of ternary mixture carrier solvents under laminar flow conditions
Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 27(3) 259 - 264, 2011, Scientific journal - Derivatization of a protein with fluorescamine utilizing the tube radial distribution phenomenon of ternary mixed carrier solvents in a capillary tube
Yuji Masuhara; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Chemistry Letters, CHEMICAL SOC JAPAN, 40(8) 804 - 805, 2011, Scientific journal - Temperature effect on separation performance in capillary chromatography based on tube radial distribution of aqueous-organic mixture carrier solvents
Jinno Naoya; Hashimoto Masahiko; Tsukagoshi Kazuhiko
The Science and engineering review of Doshisha University, Alternative:Science and Engineering Research Institute of Doshisha University, 51(3) 168 - 172, Oct. 2010 - Introduction of fluorescence and chemiluminescence detection to capillary chromatography based on tube radial distribution of water-hydrophilic- hydrophobic organic mixture carrier solvents
Seiji Ishimoto; Yudai Kudo; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Methods, ROYAL SOC CHEMISTRY, 2(9) 1377 - 1381, Sep. 2010, Scientific journal - Capillary electrophoresis with absorption/chemiluminescence dual detection system using a poyl(tetrafluoroethylene) separation capillary
Tanaka Takayuki; Jinno Naoya; Hashimoto Masahiko; Tsukagoshi Kazuhiko
The Science and engineering review of Doshisha University, Alternative:Science and Engineering Research Institute of Doshisha University, 51(2) 77 - 81, Jul. 2010 - Analytical conditions and separation performance of capillary chromatography based on the tube radial distribution of aqueous-organic mixture carrier solvents under laminar-flow conditions
Naoya Jinno; Mari Murakami; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 26(7) 737 - 742, Jul. 2010, Scientific journal - Separation of optical isomers in capillary chromatography using a poly(tetrafluoroethylene) capillary tube and an aqueous-organic mixture carrier solution
Seiji Ishimoto; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 26(6) 641 - 643, Jun. 2010, Scientific journal - Capillary chromatography based on tube radial distribution of a water-acetonitrile-ethyl acetate mixture carrier solvents: introduction of fluorescence and chemiluminescence detection
Seiji Ishimoto; Yudai Kudo; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
LUMINESCENCE, JOHN WILEY & SONS LTD, 25(2) 178 - 179, Apr. 2010 - Capillary chromatography based on tube radial distribution of aqueous-organic mixture carrier solvents: Introduction of double tubes having different inner diameters to the system
Koju Yamada; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 26(4) 507 - 510, Apr. 2010, Scientific journal - Preparation of zinc oxide ceramics with a sustainable antibacterial activity under dark conditions
Ken Hirota; Maiko Sugimoto; Masaki Kato; Kazuhiko Tsukagoshi; Tooru Tanigawa; Hiroshi Sugimoto
Ceramics International, ELSEVIER SCI LTD, 36(2) 497 - 506, Mar. 2010, Scientific journal - Synthesis of new antibacterial ZnO powders by hydrothermal treatment and their characterization
Sho Matsuoka; Masaki Kato; Ken Hirota; Kazuhiko Tsukagoshi
Funtai Oyobi Fummatsu Yakin/Journal of the Japan Society of Powder and Powder Metallurgy, Japan Society of Powder and Powder Metallurgy, 57(2) 106 - 111, Feb. 2010, Scientific journal - Capillary gel electrophoresis for ligase detection reaction products
Hashimoto Masahiko; Kamigori Jun; Tsukagoshi Kazuhiko
The Science and engineering review of Doshisha University, Alternative:Science and Engineering Research Institute of Doshisha University, 50(4) 164 - 170, Jan. 2010 - Direct detection of mutant DNA in a mixed population of higher copy number wild-type DNA based on ligase detection reaction in conjunction with fluorescence resonance energy transfer
Masahiko Hashimoto; Kazuma Yoshida; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 26(12) 1255 - 1259, 2010, Scientific journal - Distribution of fluorescent dyes dissolved in ternary mixed solvent in a microchannel under laminar flow conditions
Mari Murakami; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Chemistry Letters, CHEMICAL SOC JAPAN, 39(3) 272 - 273, 2010, Scientific journal - Elution behavior of proteins in capillary chromatography using an untreated fused-silica capillary tube and a water-hydrophilic-hydrophobic organic mixture carrier solvent
Yuji Masuhara; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Chemistry Letters, CHEMICAL SOC JAPAN, 39(7) 688 - 689, 2010, Scientific journal - Capillary chromatography based on tube radial distribution of water-acetonitrile-ethyl acetate mixture carrier solvents under laminar flow conditions
Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
ACS National Meeting Book of Abstracts, AMER CHEMICAL SOC, 240, 2010, International conference proceedings - Capillary chromatography based on tube radial distribution of aqueous-organic mixture carrier solvents: Introduction of inner-wall-modified capillary tubes
Naoya Jinno; Katsuya Tsuji; Kaoru Shikatani; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Journal of Separation Science, WILEY-V C H VERLAG GMBH, 32(23-24) 4096 - 4100, Dec. 2009, Scientific journal - Capillary chromatography based on the tube radial distribution of aqueous-organic mixture carrier solvents: Effects of the inner-wall characteristics of the fused-silica tube on separation performance
Naoya Jinno; Ko Hashimoto; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 25(11) 1369 - 1371, Nov. 2009, Scientific journal - Capillary chromatography based on tube radial distribution of aqueous-organic mixture carrier solvents
Naoya Jinno; Minoru Itano; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Talanta, ELSEVIER SCIENCE BV, 79(5) 1348 - 1353, 15 Oct. 2009, Scientific journal - Metal ion analysis using microchip CE with chemiluminescence detection based on 1,10-phenanthroline-hydrogen peroxide reaction
Takahiro Nogami; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Journal of Separation Science, WILEY-V C H VERLAG GMBH, 32(3) 408 - 412, Feb. 2009, Scientific journal - Chemiluminescence from singlet oxygen that was detected at two wavelengths and effects of biomolecules on it
Yusuke Harada; Kosuke Suzuki; Masahiko Hashimoto; Kazuhiko Tsukagoshi; Hideshi Kimoto
Talanta, ELSEVIER SCIENCE BV, 77(3) 1223 - 1227, 15 Jan. 2009, Scientific journal - Development of capillary electrophoresis equipped with a novel PTFE cell for chemiluminescence detection
Yanagiuchi Misa; Suzuki Kosuke; Hashimoto Masahiko; Tsukagoshi Kazuhiko
The Science and engineering review of Doshisha University, Alternative:Science and Engineering Research Institute of Doshisha University, 49(4) 187 - 192, Jan. 2009 - Micro-Flow separation system using an open capillary tube that works under laminar flow conditions
Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 25(2) 145 - 147, 2009, Scientific journal - Capillary chromatography based on tube radial distribution of aqueous-organic mixture carrier solvents: Elution behavior of carboxylated polymer particles in the system
Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi
Journal of Chemical Engineering of Japan, SOC CHEMICAL ENG JAPAN, 42(10) 767 - 770, 2009, Scientific journal - Development of a novel chemiluminescence analysis using liquid-liquid interface micro-reaction space in a micro-channel
Masahiko Hashimoto; Kazuhiko Tsukagoshi
Bunseki Kagaku, JAPAN SOC ANALYTICAL CHEMISTRY, 58(6) 495 - 506, 2009, Scientific journal - Analysis of antioxidants by microchip capillary electrophoresis with chemiluminescence detection based on luminol reaction
Kazuhiko Tsukagoshi; Takahiro Saito; Riichiro Nakajima
Talanta, ELSEVIER SCIENCE BV, 77(2) 514 - 517, 15 Dec. 2008, Scientific journal - Micro-channel chemiluminescence analysis using a peroxyoxalate reaction that works through liquid-liquid interface collapse under laminar-flow conditions
Kazuhiko Tsukagoshi; Yoshiyuki Hattori; Teruki Hayashi; Riichiro Nakajima; Kenichi Yamashita; Hideaki Maeda
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 24(11) 1393 - 1398, Nov. 2008, Scientific journal - Analysis by Microchip Electrophoresis
HASHIMOTO Masahiko; TSUKAGOSHI Kazuhiko
ぶんせき, 日本分析化学会, (406) 544 - 550, 05 Oct. 2008 - Micro-flow system comprised of a fused-silica capillary and chemiluminescence detection that works under laminar flow conditions
Kazuhiko Tsukagoshi; Shingo Ishida; Riichiro Nakajima
Journal of Chemical Engineering of Japan, SOC CHEMICAL ENG JAPAN, 41(2) 130 - 137, 2008, Scientific journal - Specific chemiluminescence from singlet oxygen generated by the reaction of acetonitrile and hydrogen peroxide in the presence of alkali halide
Kosuke Suzuki; Hirotaka Saito; Naoya Jinno; Masahiko Hashimoto; Kazuhiko Tsukagoshi; Hideshi Kimoto
Chemistry Letters, CHEMICAL SOC JAPAN, 37(10) 1090 - 1091, 2008, Scientific journal - Effect of saliva on measurement of chemiluminescence by a micro-reactor incorporating a micro-channel
Tsukagoshi Kazuhiko; Fukumoto Kazuaki; Nakajima Riichiro; Yamashita Kenichi; Maeda Hideaki
The Science and engineering review of Doshisha University, Alternative:Science and Engineering Research Institute of Doshisha University, 48(3) 134 - 139, Oct. 2007 - Development of an immune microanalysis system by use of peroxyoxalate chemiluminescence detection
Kazuhiko Tsukagoshi; Kazumasa Tsuge; Riichiro Nakajima
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 23(6) 739 - 741, Jun. 2007, Scientific journal - Analysis of antioxidants using a capillary electrophoresis with chemiluminescence detection system
Kazuhiko Tsukagoshi; Takehito Taniguchi; Riichiro Nakajima
Analytica Chimica Acta, ELSEVIER, 589(1) 66 - 70, Apr. 2007, Scientific journal - Characterization of chemiluminescence from singlet oxygen under laminar flow conditions in a micro-channel and its quenching with beverages
Kazuhiko Tsukagoshi; Kazuaki Fukumoto; Riichiro Nakajima; Kenichi Yamashita; Hideaki Maeda
Talanta, ELSEVIER SCIENCE BV, 72(2) 607 - 611, Apr. 2007, Scientific journal - Migration behavior of isoluminol isothiocyanate-labeled α-amino acids in capillary electrophoresis with an absorption/chemiluminescence dual detection system
Kazuhiko Tsukagoshi; Kaori Sawanoi; Riichiro Nakajima
Journal of Chromatography A, ELSEVIER SCIENCE BV, 1143(1-2) 288 - 290, 02 Mar. 2007, Scientific journal - Enhancing effect of phenylboronic acid compounds and their interactions with the diol groups of saccharides in a capillary electrophoresis- chemiluminescence detection system
Kazuhiko Tsukagoshi; Futoshi Ueno; Riichiro Nakajima; Koji Araki
Analytical Sciences, JAPAN SOC ANALYTICAL CHEMISTRY, 23(2) 227 - 230, Feb. 2007, Scientific journal - Compact polytetrafluoroethylene assembly-type capillary electrophoresis with chemiluminescence detection
Kazuhiko Tsukagoshi; Shingo Ishida; Yuichi Oda; Keiichi Noda; Riichiro Nakajima
Journal of Chromatography A, ELSEVIER SCIENCE BV, 1125(1) 144 - 146, 25 Aug. 2006, Scientific journal - Molecular recognition of mono- and disaccharides through interaction with p-iodophenylboronic acid in capillary electrophoresis with a chemiluminescence detection system
Kazuhiko Tsukagoshi; Kyohei Matsumoto; Futoshi Ueno; Keiichi Noda; Riichiro Nakajima; Koji Araki
Journal of Chromatography A, ELSEVIER, 1123(1) 106 - 112, 04 Aug. 2006, Scientific journal - Effect of Additives in the Running Buffer on α-Amino Acids Analysis by Use of Capillary Electrophoresis-Chemiluminescence Detection System
Tsukagoshi Kazuhiko; Nakahama Koji; Noda Keiichi; Nakajima Riichiro; Fukuoka Takao
The Science and engineering review of Doshisha University, Doshisha University, 47(2) 62 - 70, Jul. 2006 - Chemiluminescence from singlet oxygen under laminar flow condition in a micro-channel
Kazuhiko Tsukagoshi; Kazuaki Fukumoto; Keiichi Noda; Riichiro Nakajima; Kenichi Yamashita; Hideaki Maeda
Analytica Chimica Acta, ELSEVIER SCIENCE BV, 570(2) 202 - 206, 16 Jun. 2006, Scientific journal - Observation of the complex formation between Cu(II) and protein by capillary electrophoretic system incorporating an UV/CL dual detector
Kazuhiko Tsukagoshi; Kaori Sawanoi; Riichiro Nakajima
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 833(2) 174 - 178, Apr. 2006, Scientific journal - Capillary electrophoretic system incorporating an UV/CL dual detector
Kazuhiko Tsukagoshi; Kaori Sawanoi; Riichiro Nakajima
Talanta, 68(4) 1071 - 1075, 15 Feb. 2006, Scientific journal - Capillary electrophoresis apparatus equipped with a bioluminescence detector using a batch- or flow-type detection cell
Kazuhiko Tsukagoshi; Masayuki Tahira; Riichiro Nakajima
Journal of Chromatography A, 1094(1-2) 192 - 195, 11 Nov. 2005, Scientific journal - Separation performance in a spirally wound Teflon capillary tube
Kazuhiko Tsukagoshi; Futoshi Yonamine; Yasutaka Asamoto; Yasunori Hosohata; Riichiro Nakajima
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 230 U274 - U275, Aug. 2005 - Synthesis and fluorescence properties of 2,6-diaryl-4-(2-substituted thienyl-5-yl)pyridines
Riichiro Nakajima; Yuhei Tsurumoto; Kazuaki Mima; Takashi Tamura; Kazuhiko Tsukagoshi
Phosphorus, Sulfur and Silicon and the Related Elements, 180(5-6) 1477 - 1478, May 2005, International conference proceedings - Development of a micro total analysis system incorporating chemiluminescence detection and application to detection of cancer markers
Kazuhiko Tsukagoshi; Naoya Jinno; Riichiro Nakajima
Analytical Chemistry, 77(6) 1684 - 1688, 15 Mar. 2005, Scientific journal - Chemiluminescence Detection for Capillary Electrophoresis and Liquid Chromatography. A Review
Tsukagoshi Kazuhiko
The Science and engineering review of Doshisha University, Doshisha University, 45(4) 168 - 186, Jan. 2005 - Competitive immunoassay using capillary electrophoresis with a chemiluminescence detector
Kazuhiko Tsukagoshi; Naoya Jinno; Kae Toguchi; Riichiro Nakajima
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 78(10) 1791 - 1794, 2005, Scientific journal - Peak formation due to chemiluminescence reaction through the collapse of laminar flow liquid-liquid interface in a microreactor
Kazuhiko Tsukagoshi; Keiichi Ikegami; Riichiro Nakajima; Kenichi Yamashita; Hedeaki Maeda
Chemistry Letters, The Chemical Society of Japan, 33(9) 1178 - 1179, 05 Sep. 2004, Scientific journal - Capillary electrophoresis with chemiluminescence detector using on-capillary detection
Kazuhiko Tsukagoshi; Tatsuya Fujii; Riichiro Nakajima
Chemistry Letters, The Chemical Society of Japan, 33(8) 1000 - 1001, 05 Aug. 2004, Scientific journal - Direct detection of biomolecules in a capillary electrophoresis- chemiluminescence detection system
Kazuhiko Tsukagoshi; Koji Nakahama; Riichiro Nakajima
Analytical Chemistry, 76(15) 4410 - 4415, 01 Aug. 2004, Scientific journal - Simultaneous operation of plural separation modes in capillary electrophoresis with a chemiluminescence detector possessing a micro-space area for reaction/detection
Kazuhiko Tsukagoshi; Takashi Tokunaga; Riichiro Nakajima
Journal of Chromatography A, 1043(2) 333 - 335, 23 Jul. 2004, Scientific journal - Preparation of an iminodiacetic acid-modified capillary and its performance in capillary liquid chromatography and immobilized metal chelate affinity capillary electrophoresis
Kazuhiko Tsukagoshi; Yasushi Shimadzu; Tatsuhiko Yamane; Riichiro Nakajima
Journal of Chromatography A, 1040(1) 151 - 154, 18 Jun. 2004, Scientific journal - Capillary Electrophoresis-Chemiluminescence Detection System Equipped with a Consecutive Sample-Injection Device
Kazuhiko Tsukagoshi; Koji Nakahama; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 20(2) 379 - 381, Feb. 2004, Scientific journal - Selective detection of human serum albumin using a fused-silica capillary modified with anti-human serum albumin
Kazuhiko Tsukagoshi; Hirofumi Indou; Kaori Sawanoi; Takako Oguni; Riichiro Nakajima
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 77(7) 1353 - 1357, 2004, Scientific journal - Analytical Performance of Capillary Electrophoretic System with UV/CL or FL/CL Dual Detector
Kazuhiko Tsukagoshi; Kaori Sawanoi; Masayuki Kamekawa; Riichiro Nakajima
Chemistry Letters, The Chemical Society of Japan, 32(10) 894 - 895, 05 Oct. 2003, Scientific journal - Simultaneous analysis of plural samples in a CE-CL detector possessing micro-space area for reaction/detection
Kazuhiko Tsukagoshi; Keiichi Ikegami; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 19(9) 1339 - 1340, 01 Sep. 2003, Scientific journal - α-amino acids analysis by capillary electrophoresis with chemiluminescence detector using luminol-hydrogen peroxide-Cu(II) system
Kazuhiko Tsukagoshi; Koji Nakahama; Riichiro Nakajima
Chemistry Letters, The Chemical Society of Japan, 32(7) 634 - 635, 05 Jul. 2003, Scientific journal - Development of FIA equipped with a chemiluminescence detector using a mixed reagent of luminol and 1,10-phenanthroline
Kazuhiko Tsukagoshi; Masayuki Tahira; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 19(7) 1019 - 1023, 01 Jul. 2003, Scientific journal - Development of ultra-micro flow analysis with chemiluminescence detector
Kazuhiko Tsukagoshi; Keiko Taga; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 19(7) 977 - 978, 01 Jul. 2003, Scientific journal - Development of capillary electrophoresis - Chemiluminescence detection system
Kazuhiko Tsukagoshi
Bunseki Kagaku, The Japan Society for Analytical Chemistry, 52(1) 1 - 13, Jan. 2003, Scientific journal - Separation and determination of phenolic compounds by capillary electrophoresis with chemiluminescence detection
Kazuhiko Tsukagoshi; Takahide Kameda; Masayo Yamamoto; Riichiro Nakajima
Journal of Chromatography A, 978(1-2) 213 - 220, 29 Nov. 2002, Scientific journal - Analysis of a biopolymer by capillary electrophoresis with a chemiluminescence detector using a polymer solution as the separation medium
Kazuhiko Tsukagoshi; Yukihiro Shikata; Riichiro Nakajima; Masaharu Murata; Mizuo Maeda
Analytical Sciences, The Japan Society for Analytical Chemistry, 18(11) 1195 - 1198, 01 Nov. 2002, Scientific journal - Small-sized capillary electrophoresis with a chemiluminescence detector equipped with cross-intersection for sample injection
Kazuhiko Tsukagoshi; Takaji Suzuki; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 18(11) 1279 - 1280, 01 Nov. 2002, Scientific journal - Miniaturization of batch- and flow-type chemiluminescence detectors in capillary electrophoresis
Kazuhiko Tsukagoshi; Yuki Obata; Riichiro Nakajima
Journal of Chromatography A, 971(1-2) 255 - 260, 20 Sep. 2002, Scientific journal - Batch-type chemiluminescence detection cell for sensitization and simplification of capillary electrophoresis
Kazuhiko Tsukagoshi; Takeshi Nakamura; Riichiro Nakajima
Analytical Chemistry, 74(16) 4109 - 4116, 15 Aug. 2002, Scientific journal - Separation and determination of emetine dithiocarbamate metal complexes by capillary electrophoresis with chemiluminescence detection of the tris(2,2′-bipyridine)-ruthenium(II) complex
Kazuhiko Tsukagoshi; Noriyoshi Okuzono; Riichiro Nakajima
Journal of Chromatography A, 958(1-2) 283 - 289, 07 Jun. 2002, Scientific journal - Application of capillary electrophoresis with sensitive detection to analysis for saccharide molecules
Kazuhiko Tsukagoshi; Yuki Obata; Takaaki Kanamori; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 18(6) 709 - 710, 2002, Scientific journal - Consideration on peak shape in a batch-type chemiluminescence detection cell for capillary electrophoresis
Kazuhiko Tsukagoshi; Miwa Otsuka; Yukihiro Shikata; Riichiro Nakajima
Journal of Chromatography A, 930(1-2) 165 - 169, 28 Sep. 2001, Scientific journal - Sensitive determination of metal ions by liquid chromatography with tris(2,2′-bipyridine) ruthenium (II) complex electrogenerated chemiluminescence detection
Kazuhiko Tsukagoshi; Kazuhide Miyamoto; Riichiro Nakajima; Naoki Ouchiyam
Journal of Chromatography A, 919(2) 331 - 337, 15 Jun. 2001, Scientific journal - Application of capillary electrophoresis-chemiluminescene detection to immunoassay.
Tsukagoshi Kazuhiko
SEIBUTSU BUTSURI KAGAKU, Japanese Electrophoresis Society, 45(2) 111 - 115, 2001 - Migration behavior of the polymer particles possessing carboxylate and borate moieties in capillary electrophoresis
Kazuhiko Tsukagoshi; Akinobu Yamaguchi; Nobuo Morihara; Riichiro Nakajima; Masaharu Murata; Mizuo Maeda
Chemistry Letters, The Chemical Society of Japan, 2001(9) 926 - 927, 2001, Scientific journal - Influence of silicon membrane interposed between glass plates on microchip capillary electrophoresis with a chemiluminescence detector
Kazuhiko Tsukagoshi; Masahiko Hashimoto; Takaji Suzuki; Riichiro Nakajima; Akihiro Arai
Analytical Sciences, The Japan Society for Analytical Chemistry, 17(9) 1129 - 1131, 2001, Scientific journal - Improvement of a capillary electrophoresis-chemiluminescence detection system for using a polyacrylamide-coated capillary
K. Tsukagoshi; T. Kimura; T. Fuji; R. Nakajima; A. Arai
Analytical Sciences, The Japan Society for Analytical Chemistry, 17(2) 345 - 347, 2001, Scientific journal - Capillary electrophoresis with chemiluminescent detection for luminol using potassium ferricyanide as a catalyst
K. Tsukagoshi; Y. Ouji; R. Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 17(8) 1003 - 1005, 2001, Scientific journal - Enhanced chemiluminescence of bis(2,4,6-trichlorophenyl) oxalate-hydrogen peroxide-dyestuff system in the presence of polymer particles
Kazuhiko Tsukagoshi; Yuji Tomita; Riichiro Nakajima
Chemistry Letters, The Chemical Society of Japan, 2001(6) 486 - 487, 2001, Scientific journal - Direct observation of extraction of Cu(II) into microcapsules containing 8-quinolinol by use of capillary and chemiluminescence detection
Kazuhiko Tsukagoshi; Yukiteru Ouji; Riichiro Nakajima
Chemistry Letters, The Chemical Society of Japan, 2001(6) 528 - 529, 2001, Scientific journal - Chapter 9 Imprinting polymerisation for recognition and separation of metal ions
Kazuhiko Tsukagoshi; Masaharu Murata; Mizuo Maeda
Techniques and Instrumentation in Analytical Chemistry, 23(C) 245 - 269, 2001, In book - キャピラリー電気泳動-化学発光検出法の免疫分析への応用 : 色素包括リポソーム標識剤の化学発光検出
塚越 一彦; 中島 理一郎
生物物理化学 = Journal of Electrophoresis, 44 3 - 3, 15 Oct. 2000 - Determination of α-Amino Acid Using a 1,10-Phenanthroline-Hydrogen Peroxide-Osmium(VIII) Chemiluminescence
TSUKAGOSHI Kazuhiko; FUKUOKA Takao; NAKAJIMA Riichiro; HARA Tadashi
The Science and engineering review of Doshisha University, Doshisha University, 41(3) 180 - 184, Oct. 2000 - Immunoassay using chemiluminescence detection of dyestuff-containing liposomes as a labeling reagent
Kazuhiko Tsukagoshi; Hideshi Akasaka; Yasuo Okumura; Ryosuke Fukaya; Miwa Otsuka; Kazutoshi Fujiwara; Hiroyuki Umehara; Reiko Maeda; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 16(2) 121 - 124, Feb. 2000, Scientific journal - Microchip capillary electrophoresis using on-line chemiluminescence detection
Masahiko Hashimoto; Kazuhiko Tsukagoshi; Riichiro Nakajima; Kazuo Kondo; Akihiro Arai
Journal of Chromatography A, 867(1-2) 271 - 279, 21 Jan. 2000, Scientific journal - Simple and sensitive detection cell for capillary electrophoresis-chemiluminescence analysis using peroxyoxalate reagent
Kazuhiko Tsukagoshi; Miwa Otsuka; Masahiko Hashimoto; Riichiro Nakajima; Hideshi Kimoto
Chemistry Letters, The Chemical Society of Japan, 29(2) 98 - 99, 2000, Scientific journal - Application of microchip capillary electrophoresis with chemiluminescence detection to an analysis for transition-metal ions
Kazuhiko Tsukagoshi; Masahiko Hashimoto; Riichiro Nakajima; Akihiro Arai
Analytical Sciences, The Japan Society for Analytical Chemistry, 16(11) 1111 - 1112, 2000, Scientific journal - Double-featured chemiluminescence reagent prepared through a mixing procedure and its application to the detection of heme protein
K. Tsukagoshi; M. Yamamoto; R. Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 16(12) 1357 - 1359, 2000 - Batch-type detection cell using a peroxyoxalate chemiluminescence system for capillary electrophoresis
Kazuhiko Tsukagoshi; Miwa Otsuka; Masahiko Hashimoto; Riichiro Nakajima; Kazuo Kondo
Analytical Sciences, The Japan Society for Analytical Chemistry, 15(12) 1257 - 1260, Dec. 1999, Scientific journal - Flow-type chemiluminescence detection cell using an optical fiber for capillary electrophoresis
Masahiko Hashimoto; Takeshi Nakamura; Kazuhiko Tsukagoshi; Riichiro Nakajima; Kazuo Kondo
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 72(12) 2673 - 2679, Dec. 1999, Scientific journal - Design of a pressure-mobilization system for capillary isoelectric focusing-chemiluminescence detection
Masahiko Hashimoto; Kazuhiko Tsukagoshi; Riichiro Nakajima; Kazuo Kondo
Analytical Sciences, The Japan Society for Analytical Chemistry, 15(12) 1281 - 1284, Dec. 1999, Scientific journal - Simple and convenient cell for chemiluminescence detection in capillary electrophoresis
Kazuhiko Tsukagoshi; Takeshi Nakamura; Masahiko Hashimoto; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 15(11) 1047 - 1048, Nov. 1999, Scientific journal - Chemiluminescence detection of heme proteins separated by capillary isoelectric focusing
Masahiko Hashimoto; Kazuhiko Tsukagoshi; Riichiro Nakajima; Kazuo Kondo
Journal of Chromatography A, 852(2) 597 - 601, 13 Aug. 1999, Scientific journal - Compact detection cell using optical fiber for sensitization and simplification of capillary electrophoresis-chemiluminescence detection
Masahiko Hashimoto; Kazuhiko Tsukagoshi; Riichiro Nakajima; Kazuo Kondo
Journal of Chromatography A, 832(1-2) 191 - 202, 05 Jan. 1999, Scientific journal - Consideration on Lebeling of Bovine Serum Albumin with Rhodamine B Isothiocyanate by the Use of Electropherogram
HASHIMOTO Masahiko; TSUKAGOSHI Kazuhiko; NAKAJIMA Riichirou; KONDO Kazuo
The science and engineering review of Doshisha University, Doshisha University, 39(4) 15 - 18, Jan. 1999 - Chemiluminescence detection in microchip capillary electrophoresis
Masahiko Hashimoto; Kazuhiko Tsukagoshi; Riichiro Nakajima; Kazuo Kondo; Akihiro Arai
Chemistry Letters, The Chemical Society of Japan, 28(8) 781 - 782, 1999, Scientific journal - Basic Study on Electropherogram of Bovine Serum Albumin Denatured by Heating
TSUKAGOSHI Kazuhiko; HASHIMOTO Masahiko; NAKAJIMA Riichirou; KONDO Kazuo
The science and engineering review of Doshisha University, Doshisha University, 39(3) 1 - 6, Oct. 1998 - Migration behavior of dyestuff-containing liposomes in capillary electrophoresis with chemiluminescence detection
Kazuhiko Tsukagoshi; Yasuo Okumura; Riichiro Nakajima
Journal of Chromatography A, 813(2) 402 - 407, 17 Jul. 1998, Scientific journal - Surface Imprinting: Preparation of Metal Ion-Imprinted Resins by Use of Complexation at the Aqueous-Organic Interface
Kazuhiko Tsukagoshi; Kai Yu Yu; Yoshihisa Ozaki; Tohru Miyajima; Mizuo Maeda; Makoto Takagi
ACS Symposium Series, 703 251 - 263, 1998, Scientific journal - Chemiluminescence Property of the Luminol-Hydrogen Peroxide-Copper(II) System in the Presence of Surface-Carboxylated Microspheres
Kazuhiko Tsukagoshi; Masayuki Sumiyama; Riichiro Nakajima; Masamichi Nakayama; Mizuo Maeda
Analytical Sciences, The Japan Society for Analytical Chemistry, 14(2) 409 - 412, 1998, Scientific journal - Separation behavior of biological constituents having cis-diol groups through interactions with phenylboronic acid sites introduced on the inner wall of a fused-silica capillary
Kazuhiko Tsukagoshi
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 71(12) 2831 - 2836, 1998, Scientific journal - 田辺製薬(株)分析研究所を訪ねて
塚越 一彦
ぶんせき, 日本分析化学会, 265 59 - 61, 05 Jan. 1997 - High-Sensitivity Determination of Emetine Dithiocarbamate Copper(II) Complex Using the Electrogenerated Chemiluminescence Detection of Tris(2,2′-bipyridine)ruthenium(II)
Kazuhiko Tsukagoshi; Kazuhide Miyamoto; Eiji Saiko; Riichiro Nakajima; Tadashi Hara; Kaoru Fujinaga
Analytical Sciences, The Japan Society for Analytical Chemistry, 13(4) 639 - 642, 1997, Scientific journal - Preparation of Phenylboronic Acid-Modified Capillary and Separation of Nucleosides by Capillary Electrophoresis
Kazuhiko Tsukagoshi; Masahiko Hashimoto; Kazuma Ichien; Shoukun Gen; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 13(3) 485 - 487, 1997, Scientific journal - Electrophoretic Separation and High-Sensitivity Detection of Dyestuff-Labeled Proteins Using an Untreated Fused-Silica Capillary and Sodium Dodecyl Sulfate-Containing Buffer for Migration and Labeling
Kazuhiko Tsukagoshi; Masahiko Hashimoto; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 13(4) 565 - 570, 1997, Scientific journal - High-Sensitive Analysis of Herne Proteins Separated by Capillary Electrophoresis with On-Line Chemiluminescence Detection Using a Luminol and Hydrogen Peroxide System
Kazuhiko Tsukagoshi; Shigehiro Fujimura; Riichiro Nakajima
Analytical Sciences, The Japan Society for Analytical Chemistry, 13(2) 279 - 281, 1997, Scientific journal - 鋳型重合の新しい展開 : 界面を利用する機能性の創出
塚越 一彦
同志社工学会報, 同志社大学, 38 43 - 55, Dec. 1996 - Metal-ion imprinted resin prepared using an interaction at the aqueous-organic interface and its characterization
Kazuhiko Tsukagoshi; Mizuo Maeda; Makoto Takagi
Bunseki Kagaku, The Japan Society for Analytical Chemistry, 45(11) 975 - 986, Nov. 1996, Scientific journal - インターネット上で提供されている分析化学情報
田村 隆; 塚越 一彦; 中島 理一郎
ぶんせき, 日本分析化学会, 262 840 - 844, 05 Oct. 1996 - Electrophoretic Behavior of Dyestuff-Containing Liposome in a Capillary with Original On-Line Chemiluminescence Detection
Kazuhiko Tsukagoshi; Yasuo Okumura; Hideshi Akasaka; Riichiro Nakajima; Tadashi Hara
Analytical Sciences, The Japan Society for Analytical Chemistry, 12(6) 869 - 874, 1996, Scientific journal - Performance of a Coiled Capillary of One-cm Diameter in Capillary Electrophoresis
Kazuhiko Tsukagoshi; Ayumi Kenmochi; Riichiro Nakajima; Tadashi Hara
Analytical Sciences, The Japan Society for Analytical Chemistry, 12(5) 811 - 814, 1996, Scientific journal - Preparation and Characterization of Polymer Microspheres Which Have Specific Binding Ability for Saccharide Molecules
Kazuhiko Tsukagoshi; Rika Kawasaki; Mizuo Maeda; Makoto Takagi
Analytical Sciences, The Japan Society for Analytical Chemistry, 12(5) 721 - 726, 1996, Scientific journal - Characterization of dyestuff-containing liposome by capillary zone electrophoresis using on-line chemiluminescence detection
Kazuhiko Tsukagoshi; Hideshi Akasaka; Riichiro Nakajima; Tadashi Hara
Chemistry Letters, The Chemical Society of Japan, 25(6) 467 - 468, 1996, Scientific journal - On-Line Capillary Zone Electrophoretic Separation-Chemiluminescence Detection of Protein Labeled with Fluorescamine
Kazuhiko Tsukagoshi; Akiko Tanaka; Riichiro Nakajima; Tadashi Hara
Analytical Sciences, The Japan Society for Analytical Chemistry, 12(3) 525 - 528, 1996, Scientific journal - Surface Imprinting. Characterization of a Latex Resin and the Origin of the Imprinting Effect
Tsukagoshi Kazuhiko; Yu Kai Yu; Maeda Mizuo; Takagi Makoto; Miyajima Tohru
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 68(11) 3095 - 3103, 1995 - Preparation and Sugar Binding Property of Microspheres Having Surface-anchored Phenylboronic Acid Groups
TSUKAGOSHI Kazuhiko; KAWASAKI Rika; MAEDA Mizuo; TAKAGI Makoto
Chemistry Letters, The Chemical Society of Japan, 1994(4) 681 - 684, 05 Apr. 1994 - 分子認識ホスト合成法としての鋳型重合の新しい展開
塚越 一彦
化学と工業 = Chemistry and chemical industry, 47(4) 555 - 556, 01 Apr. 1994 - Separation and Determination of Trace Dinitropyrenes by Means of Off-Line Reduction-HPLC-Chemihiminescence Detection. Application to Assessing Atmospheric Environment
Mizuo Maeda; Kazuhiko Tsukagoshi; Masaharu Murata; Makoto Takagi; Toshiro Yamashita
Analytical Sciences, The Japan Society for Analytical Chemistry, 10(4) 583 - 587, 1994, Scientific journal - Letters to the Editor Template-Dependent Metal Adsorptivity of Dialkyl Phosphate-Type Resins Prepared by Surface Template Polymerization Technique
Mizuo Maeda; Masaharu Murata; Kazuhiko Tsukagoshi; Makoto Takagi
Analytical Sciences, The Japan Society for Analytical Chemistry, 10(1) 113 - 115, 1994 - キャピラリー電気泳動 - レーザー励起蛍光検出による糖類の分離・分析
塚越 一彦
ぶんせき, 227 903 - 904, 05 Nov. 1993 - 第2回アジア分析化学会議 (Asianalysis II)
塚越 一彦; 高木 誠
ぶんせき, 日本分析化学会, 227 907 - 909, 05 Nov. 1993 - Metal Ion-Selective Adsorbent Prepared by Surface-Imprinting Polymerization.
Tsukagoshi Kazuhiko; Yu Kai Yu; Maeda Mizuo; Takagi Makoto
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 66(1) 114 - 120, 1993 - Adsorption Behavior of Metal Ions onto Co(II)-Imprinted Microspheres Prepared by Surface Imprinting. Effect of Co(II)-Imprinting
Kazuhiko Tsukagoshi; Kaiyu Yu; Mizuo Maeda; Makoto Takagi
Kobunshi Ronbunshu, The Society of Polymer Science, Japan, 50(5) 455 - 458, 1993, Scientific journal - Metal-Ion Complexation Equilibria of Ion-Exchange Resins Prepared by a Surface Template Polymerization
Hirotsugu Kido; Hidehiro Sonoda; Kazuhiko Tsukagoshi; Mizuo Maeda; Makoto Takagi; Hideshi Maki; Tohru Miyajima
Kobunshi Ronbunshu, The Society of Polymer Science, Japan, 50(5) 403 - 410, 1993, Scientific journal - Specific complexation of glucose with a diphenylmethane-3,3′- diboronic acid derivative: Correlation between the absolute configuration of mono- and di-saccharides and the circular dichroic activity of the complex
Yutaka Shiomi; Miwako Saisho; Kazuhiko Tsukagoshi; Seiji Shinkai
Journal of the Chemical Society, Perkin Transactions 1, (17) 2111 - 2117, 1993, Scientific journal - 分離分析用機能材としての鋳型重合樹脂 (1992年の化学-7-)
塚越 一彦; 高木 誠
化学, 化学同人, 47(7) p500 - 501, Jul. 1992 - Metal-Ion Complexation Behavior of Resins Prepared by a Novel Template Polymerization Technique.
KIDO Hirotsugu; TSUKAGOSHI Kazuhiko; MAEDA Mizuo; TAKAGI Makoto; MIYAJIMA Tohru
Analytical Sciences, The Japan Society for Analytical Chemistry, 8(6) 749 - 753, 1992 - Letters to the Editor Metal Ion-Imprinted Microspheres Prepared by Reorganization of the Coordinating Groups on the Surface
Kai Yu; I. Yu; Kazuhiko Tsukagoshi; Mizuo Maeda; Makoto Takagi
Analytical Sciences, The Japan Society for Analytical Chemistry, 8(5) 701 - 703, 1992, Scientific journal - Adsorption of metal ions onto carboxylated microspheres synthesized by seeded emulsion polymerization.
Kaiyu Yu; Hirotsugu Kioo; Kazuhiko Tsukagoshi; Mizuo Maeda; Makoto Takagi
BUNSEKI KAGAKU, The Japan Society for Analytical Chemistry, 41(9) 459 - 461, 1992, Scientific journal - Specific Complexation with Mono- and Disaccharides That Can Be Detected by Circular Dichroism
Kazuhiko Tsukagoshi; Seiji Shinkai
Journal of Organic Chemistry, 56(13) 4089 - 4091, 01 Jun. 1991, Scientific journal - Molecular recognition of mono- and di-saccharides by phenylboronic acids in solvent extraction and as a monolayer
Seiji Shinkai; Kazuhiko Tsukagoshi; Yuichi Ishikawa; Toyoki Kunitake
Journal of the Chemical Society, Chemical Communications, (15) 1039 - 1041, 1991, Scientific journal - The determination of a small amount of a biological constituent by the use of chemiluminescence. XVII. Experimental consideration on the chemiluminescence detection of protein using the 1,10-phenanthroline-hydrogen peroxide-copper(II) system
Tadashi Hara; Kazuhiko Tsukagoshi; Hideshi Kimoto; Toshihito Imai; Riichirou Nakajima
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 63(8) 2423 - 2425, Aug. 1990, Scientific journal - Chemiluminescence of L-tryptophan in copper(II)-hydrogen peroxide-potassium sodium tartrate system.
Hara Tadashi; Tsukagoshi Kazuhiko; Imai Toshihito; Yoshida Naoki; Nakajima Riichiro
Chemistry Letters, The Chemical Society of Japan, 19(6) 843 - 846, 1990 - Chemiluminescence Analyses of Biological Constituents Using Metal-Complex Catalysts A Review
Tadashi Hara; Kazuhiko Tsukagoshi
Analytical Sciences, The Japan Society for Analytical Chemistry, 6(6) 797 - 806, 1990, Scientific journal - The determination of a small amount of a biological constituent by use of chemiluminescence. XVI. High-sensitive detection of proteins by an ion-exchange chromatograph-chemiluminescence detector system
T. Hara; K. Tsukagoshi; H. Tsuji
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 63(3) 770 - 776, 1990, Scientific journal - A highly sensitive fiber-optic immunosensor using a metal-complex compound as a chemiluminescent catalyst
T. Hara; K. Tsukagoshi; A. Arai; Y. Imashiro
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 62(9) 2844 - 2848, Sep. 1989, Scientific journal - The determination of a small amount of a biological constituent by the use of chemiluminescence. XV. A zeolite column-chemiluminescence detection system for the separation and the determination of a protein mixture
T. Hara; K. Tsukagoshi; Y. Kurita
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 62(5) 1501 - 1508, 1989, Scientific journal - Improvement in FIA system for determining small amounts of proteins with chemiluminescence detection
Kazuhiko Tsukagoshi; Hideshi Kimoto; Tadashi Hara
bunseki kagaku, The Japan Society for Analytical Chemistry, 38(7) T100 - T103, 1989, Scientific journal - The determination of a small amount of biological constituent by the use of chemiluminescence. XIV. The flow-injection analysis of protein using ultrasonic chemiluminescence of luminol
Tadashi Hara; Kazuhiro Nakatsu; Akihiro Arai; Tatsunari Yoshida; Kazuhiko Tsukagoshi
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 61(8) 2996 - 2998, Aug. 1988, Scientific journal - The determination of a small amount of biological constituent by the use of chemiluminescence. XIII. High sensitive metal chelate affinity chromatography
Tadashi Hara; Kazuhiko Tsukagoshi; Tatsunari Yoshida
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 61(8) 2779 - 2783, Aug. 1988, Scientific journal - 化学発光検出を用いるコバルト(III)-ウシ血清アルブミン錯体生成の研究
塚越 一彦; 原 正
同志社大学理工学研究報告, 同志社大学理工学研究所, 29(1) p1 - 6, May 1988 - 化学発光反応を用いる微量タンパク質定量の考察--化学発光触媒活性抑制法
塚越 一彦; 原 正
同志社大学理工学研究報告, 同志社大学理工学研究所, 28(3) p179 - 189, Feb. 1988 - Determination of a small amount of a biological constituent by the use of chemiluminescence. XII. Highly sensitive immunoaffinity chromatography.
Hara Tadashi; Tsukagoshi Kazuhiko; Arai Akihiro; Iharada Takeshi
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 61(1) 301 - 303, 1988 - 多孔性シリカビ-ズへのタンパク質の吸着--化学発光検出器を用いる水溶液中のタンパク質濃度の決定
塚越 一彦
同志社大学理工学研究報告, 同志社大学理工学研究所, 28(2) p103 - 114, Nov. 1987 - The determination of a small amount of a biological constituent by the use of chemiluminescence. XI. The determination of protein using a 1,10-phenanthroline-hydrogen peroxide-osmium(VIII) system.
Hara Tadashi; Tsukagoshi Kazuhiko
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 60(6) 2031 - 2035, 1987 - The determination of a small amount of a biological constituent by the use of chemiluminescence. X The determination of protein using a 1,10-phenanthroline-hydrogen peroxide-ruthenium(III) system.
Hara Tadashi; Tsukagoshi Kazuhiko; Imaki Masakatsu
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 60(4) 1537 - 1539, 1987 - Determination of a small amount of a biological constituent by use of chemiluminescence. VIII Effect of heating on the determination of protein.
Hara Tadashi; Tsukagoshi Kazuhiko; Arai Akihiro; Iharada Takeshi
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 59(11) 3681 - 3683, 1986 - Determination of a small amount of biological constituent by use of chemiluminescence. III. The flow-injection analysis of protein by direct injection
Tadashi Hara; Motohiro Toriyama; Kazuhiko Tsukagoshi
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 57(6) 1551 - 1555, 01 Jun. 1984, Scientific journal - Immunoassay using a metal-complex compound as a chemiluminescent catalyst. III. Flow-through analysis of a labeled antigen bound by immune reaction.
Hara Tadashi; Toriyama Motohiro; Tsukagoshi Kazuhiko
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 57(2) 587 - 588, 1984 - Determination of a small amount of a biological constituent by the use of chemiluminescence. II. Determination of albumin as a model protein by means of the flow-injection analysis using a cobalt(III) complex compound as a catalyst.
Hara Tadashi; Toriyama Motohiro; Tsukagoshi Kazuhiko
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 57(1) 289 - 290, 1984 - Immunoassay using a metal-complex compound as a chemiluminescent catalyst. I. Iron(III)phthalocyanine as a labeling reagent.
Hara Tadashi; Toriyama Motohiro; Tsukagoshi Kazuhiko
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 56(8) 2267 - 2271, 1983 - Determination of a small amount of biological constituent by use of chemiluminescence. I. The flow-injection analysis of protein.
Hara Tadashi; Toriyama Motohiro; Tsukagoshi Kazuhiko
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 56(5) 1382 - 1387, 1983 - Immunoassay using a metal-complex compound as a chemiluminescent catalyst. II. An improvement of the analytical method for practical use.
Hara Tadashi; Toriyama Motohiro; Tsukagoshi Kazuhiko
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 56(10) 2965 - 2968, 1983 - 標識剤としての化学発光錯体触媒の基礎的検討
塚越 一彦; 鳥山 素弘; 原 正
同志社大学理工学研究報告, 同志社大学理工学研究所, 23(2) p119 - 129, Aug. 1982
Books etc
- Surface Imprinting. Metal-ion Imprinted Resin Prepared by Using Complexation at the Aqueous-Organic Interface
Kazuhiko Tsukagoshi, Kai Yu Yu, Yoshihisa Ozaki, Tohru Miyajima, Mizuo Maeda, and Makoto Takagi, ACS Symposium Series 703, "Molecular and Ionic Recognition with Imprinted Polymers", R. A. Bartsch and M. Maeda Eds., Chapter 17, p. 251-263, 1998. - Imprinting polymerization for recognition and separation of metal ions
Kazuhiko Tsukagoshi, Masaharu Murata, and Mizuo Maeda, "Molecularly Imprinted Polymers", B. Sellergren ed., Elsevier, Chapter 9, p. 245 - 269, 2001. - Investigation of Tube Radial Distribution Phenomenon (TRDP) and Its Function Appearance
Kazuhiko Tsukagoshi, Kimura Keibun-Sha, Chapters 1-14, pp. 1-372, September, 2015.
Research Projects
- 微小領域の流れの制御と配管チューブを分離カラムとする連続注入・分離・検出法の開発
塚越 一彦
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), 2022/04 -2024/03, Grant-in-Aid for Scientific Research (C), Doshisha University - Academic Systematization of Separation Analysis Based on Phase Separation Multiphase Flow and Its Practical Technical Improvements
塚越 一彦
様々な二相分離混合溶液を使って「相分離混相流」を創出させ、「相分離混相流」の流れの解明、特徴等を学術的視点から明らかにしていくことに取り組んだ。「相分離混相流」を様々な二相分離混合溶液で確認し、創出メカニズムを体系的に調べた。これまで主に使用してきた水-親水性／疎水性有機溶媒三成分混合溶液以外に、新たな二相分離混合溶液を取り上げ、「相分離混相流」に関わる情報のデータベース化を進めた。例として、PEG/デキストラン混合溶液を使った場合の「相分離混相流」創出について調べた。まず、PEG/デキストランの各濃度水溶液において、相図を作成し、50℃（均一一相）から25℃（不均一二相）に相変化させた時の上相/下相体積比を調べた。次に各PEG/デキストラン混合溶液（蛍光色素含有）をキャピラリーチューブに送液し、チューブを温度調整した。顕微鏡-CCDカメラを使って、チューブ内の流体流れを観察した。「相分離混相流」の一つである「管径方向分配流」を創出することができた。この流れをキャピラリーチューブ２重管に応用し、タンパク質を分離回収することができた。他の混合溶媒についても、相図作成、上相/下相体積比の確認、流れの画像観察を試みた。様々な二相分離混合溶液について、密度、粘度、表面張力、接触角等を測定しながら、相分離混相流の発生メカニズムを調べた。さらに、流れの条件、流れ特性（慣性力、粘性力等）、無次元数（ウエバー数、レイノルズ数等）、粘性散逸法則、二相混相流の線形安定性解析等の流体力学の視点から考察を深め、学術的視点からの体系化を試みた。, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), 2017/04 -2021/03, Grant-in-Aid for Scientific Research (B), Doshisha University - Clarification of specific liquid-liquid interface in a microfluidic flow and its application to analytical system
Tsukagosi Kazuhiko
Specific microfluidic flows with two-phase separation mixed solvent solutions, such as ternary water-hydrophilic/hydrophobic organic solvent, water-surfactant, water-ionic liquid, and fluorocarbon/hydrocarbon organic solvent mixtures, have been reported as a new type of multiphase flow. When homogeneous mixed solvent solutions are fed into a microspace, the solutions change from homogeneous to heterogeneous solutions featuring a liquid-liquid interface via phase transformation by changing the pressure and/or temperature. This type of flow is called “phase separation multiphase flow” (PS-MPF) and differs from conventional immiscible multiphase flows. In particular, the annular flow in a PS-MPF is interesting and useful. This flow is called a “tube radial distribution flow” (TRDF). The outline of the PS-MPF has been clarified from various viewpoints of the first research stage. New technologies were developed based on a TRDF in a PS-MPF., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), 2014/04 -2017/03, Competitive research funding, Grant-in-Aid for Scientific Research (C), Doshisha University - Development of Novel Separation Method Based on Specific Distribution Behavior of Fluidic Solvents in a Capillary Tube
TSUKAGOSHI Kazuhiko
We examined a specific microfluidic behavior exhibited by mixed-solvent solutions in a microspace, coined as the tube radial distribution phenomenon (TRDP). The specific fluidic behavior was observed in the following solution systems : ternary water-hydrophilic/hydrophobic organic solvents, water-surfactant、 water-ionic liquid, and fluorous/organic solvents. When the mixed homogeneous solutions were delivered into a microspace under certain conditions, the solvent molecules were radially distributed in the microspace, generating inner and outer phases with a kinetic liquid-liquid interface. The TRDP was fundamentally evaluated by fluorescence microscopy, phase diagrams construction, and the elution behaviors of solutes in a capillary tube. A TRDP-based capillary chromatography, referred to as tube radial distribution chromatography (TRDC), where the outer phase serves as a pseudo-stationary phase under laminar flow conditions, has been developed as one of the applications of TRDP. We have also investigated TRDP-based extraction, chemical reaction, and mixing processes, coined as tube radial distribution extraction (TRDE), tube radial distribution reaction (TRDR), and tube radial distribution mixing (TRDM), respectively. The concept and experimental findings regarding TRDP, TRDC, TRDE, TRDR, and TRDM were investigated through this study., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), 2011 -2013, Competitive research funding, Grant-in-Aid for Scientific Research (C), Doshisha University - Studies on Chemiluminescence Analysis Using Liquid-Liquid Micro-Space Interface in a Micro-Channel
TSUKAGOSHI Kazuhiko
A novel chemiluminescence analysis to taked advantage of a specific chemiluminescence reaction space around the liquid-liquid interface in a micro-channel under laminar flow conditions has been reported. We call the analytical system "micro-channel chemiluminescence analysis (MCCLA)". First, the concept of micro-channel chemiluminescence analysis and its features were described and considered through direct observations of fluorescence and chemiluminescence using a fluorescence microscope-CCD camera and a microscope CCD camera. Next, several concrete examples of micro-channel chemiluminescence analysis were introduced, and the experimental data were basically discussed. A fluorescence compound was examined by micro-channel chemiluminescence analysis using an oxalate chemiluminescence reaction. Anti-oxygen, beverages, and saliva were also investigated by chemiluminescence analysis using a singlet oxygen chemiluminescence reaction. Furthermore, we have developed a capillary chromatography using an open capillary tube and a water-hydrophilic/hydrophobic organic solvent mixture as a carrier solution ; the system works under laminar flow conditions. The capillary chromatography is called a tube radial distribution chromatography (TRDC) system., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), 2008 -2010, Grant-in-Aid for Scientific Research (C), Doshisha University - Development of Sensitive "Micro-immunoassay System" and Application to Determination of Cancer Marker
TSUKAGOSHI Kazuhiko
We developed a chip analysis system incorporating electrophoresis-chemiluminescence detection, in which the chemiluminescence reaction of isoluminol isothiocyanato (ILITC) (chemiluminescence reagent for labeling)-microperoxidase (catalyst) - hydrogen peroxide (oxidant) as well as bis[2-(3,6,9-trioxadecanyloxycarbony)-4-nitrophenyl]oxalate (TDPO) (oxalate reagent)-fluorescein isothiocyanate (FITC) (fluorescence reagent for labeling) - hydrogen peroxide (oxidant) were adopted. The analysis system performed the following three processes on a chip having micro-channels of 20 μm deep and 50 μm width: immune reaction for high selectivity, electrophoresis for formation and transportation of the sample plug, and chemiluminescence detection for high sensitivity. The three processes were compactly integrated onto the chip. As the first process, the immune reaction was performed using an antibody-immobilized glass bead. The glass bead was placed in one of the reservoirs along with antigen (analyte) and a known amount of ILITC- or FITC-labeled antigen to set up a competitive immune reaction. For electrophoresis, as the second process, the reactant after the immune reaction was fed electrophoretically into the intersection resulting in a sample plug. The sample plug was then moved into another reservoir containing hydrogen peroxide or TDPO-hydrogen peroxide mixture solution. At this point, chemiluminescence detection was performed as the third process: the labeled antigen mixed with the reagent to produce chemiluminescence. Chemiluminescence was detected with a photomultiplier tube located under the reservoir. The chip analysis system described here was capable of determining, with high selectivity and sensitivity, human serum albumin or immunosuppressive acidic protein (IAP) as a cancer marker in human serum. IAP was analyzed by immune reaction, electrophoresis, and chemiluminescence detection on the microchip. IAP was determined over the range of 1.0x10^<-7> - 5.0x10^<-6> M (with a detection limit of 1.0x10^<-7> M (S/N=3) (correlation coefficient, 0.999). One analysis was performed within 2 min., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), 2005 -2006, Competitive research funding, Grant-in-Aid for Scientific Research (C), Doshisha University - Development of high quality "capillary electrophoresis-chemiluminescence detection" system and its application to a environmental sample
TSUKAGOSHI Kazuhiko
Phenolic compounds were analyzed by means of capillary electrophoresis with chemiluminescence detector. Peroxyoxalate chemiluminescence reagent was used together with dansyl chloride as a labeling reagent. The reagent concentrations, the labeling procedures, and the performance of chemiluminescence detection cells were examined for sensitive detection of phenolic compounds. Six kinds of phenolic compounds (phenol, 2-chlorophenol, 4-chlorophenol, 2,4-dichlorophenol, 2,6-dichlorophenol, and 2,4,6-trichlorophenol) were determined over a range of 3 orders of magnitude with the detection limits of 10-7 M order; their detection limits were ca. 10 times as low as those obtained by an ordinary fluorescence detector. A running buffer solution containing SDS and acetonitrile provided satisfactory results in separation of fifteen kinds of phenolic compounds. The combination of capillary, electrophoresis with chemiluminescence detector and column concentration procedure supported the possibility that the present system could be applied to real samples such as surface and reused waters. Also, plural samples were simultaneously analyzed in the electrophoresis - chemiluminescence detector system, taking advantage of micro-space area for reaction/detection at the tip of capillary. The chemiluminescence reaction of 1,10-phenanthroline and hydrogen peroxide was adopted and Cu(II) sample was used as a model. Three different length capillaries were inserted into the flow-type chemiluminescence detection cell made of Teflon-tube. Three samples migrated in the corresponding capillaries at the same time and mixed with CL reagent at the tip of capillary to produce chemiluminescence. The simultaneous analysis of plural samples in the present system supported the possibility that the real sample could be determined more easily, rapidly, and precisely with the calibration plots., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), 2003 -2004, Competitive research funding, Grant-in-Aid for Scientific Research (C), Doshisha University - Development of simple "capillary electrophoresis - chemiluminescence detection" system and its application to a gene diagnosis
TSUKAGOSHI Kazuhiko
A simple and convenient chemiluminescence detection cell was designed for capillary electrophoresis. The detection cell easily combined with capillary electrophoresis equipment. Luminol chemiluminescence was adapted for use with the detection cell. Detailed analysis and testing of the system revealed that luminol could be determined over a range of 2.5 x 10^<-10> to 6.5 x 10^<-7> M (correlation coefficient, 0.999), with a detection limit (S/N=3) of 2.5 x 10^<-10> M (7 amol). Furthermore, each component in a mixture of glycine, glycylglycine, and glycylglycylglycine, which were labeled with isoluminol isothiocyanate, was baseline separated and sensitively detected. Moreover, the stacking procedure was applied to post column detection in capillary electrophoresis. When using acetonitrile stacking under certain conditions in the present system, chemiluminescence intensities of luminol and labeled compounds were about an order of magnitude higher than those obtained without stacking. The detection limit for luminol was 1.5 x 10^<-11> M (S/N=3), representing the highest sensitivity of luminol yet reported. Also, the effect of p-iodophenol as an enhancer of luminol chemiluminescence was examined under weak alkaline conditions. The chemiluminescence intensity of luminol was about two orders of magnitude higher than that in the unenhanced reaction. Next, we developed capillary electrophoresis with chemiluminescence detector using a polymer solution as separation medium for analysis of biopolymers, such as DNA and protein. Peroxyoxalate chemiluminescence reagent of bis (2,4,6-trichlorophenyl)oxalate was used together with fluorescein-labeling reagent. Fluorescein-labeled DNA was prepared through polymerase chain reaction using fluorescein-labeled deoxyadenosine triphosphate. A mixture of the labeled DNA fragments (500, 600, 700, 800, 900, and 993 bp) was successfully separated and detected by the present system. A mixture of proteins (lysozyme, cytochrome C, and ribonuclease A) which were labeled with fluoresein isothiocyanate was also separated and detected., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), 2001 -2002, Competitive research funding, Grant-in-Aid for Scientific Research (C), Doshisha University - Studies on super sensitive analysis with atto mole order by means of capillary electrophoresis equipped with chemiluminescence detection. -Development of high performance chemiluminescence detection cell using an optical fiber-
TSUKAGOSHI Kazuhiko
A new and simple chemiluminescence detection cell using optical fiber was designed for capillary electrophoresis. The cell was easily combined with capillary electrophoresis and the system was operated without any tedious procedures. Luminol chemiluminescence was adapted for use with this detection cell. Hydrogen peroxide and catalysts were in detail examined concerning their usage and concentration with the cell. The detection limit for luminol was 5.0 x 10^<-10> M (14 amol ; S/N = 3), which was the most sensitive result reported so far. Also, a mixture sample of glycine, glycylglycine, and glycylglycylglycine which were labeled with isoluminol isothiocyanate was subjected to the present system. They were sensitively detected and represented with complete base-line separations. The concept of chemiluminescence detection cell using optical fiber was applied for the following researches : "Chemiluminescence detection of heme proteins separated by capillary isoelectric focusing", "Batch-type detection cell using a peroxyoxalate chemiluminescence system for capillary electrophoresis", "Design of pressure-mobilization system for capillary isoelectric focusing - chemiluminescence detection", and "Flow-type chemiluminescence detection cell using optical fiber for capillary electrophoresis"., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), 1999 -2000, Grant-in-Aid for Scientific Research (C), Doshisha University - キャピラリー電気泳動-化学発光検出による特異的徴量タンパク質の分離・定量-色素包括リポソームを標識剤とする免疫分析-
塚越 一彦
色素包括リポソームでタンパク質を標識し、キャピラリー電気泳動で分離、そして化学発光検出するという新しいタンパク質の分離・検出法を検討した。標識剤としての色素包括リポソームは、泳動後キャピラリー先端での化学発光試薬(有機溶媒)と混合され、リポソーム膜の崩壊により、多量の蛍光色素を放出する。それらを化学発光検出することにより超高感度なタンパク質検出が可能となる(「増幅型」の検出)。 まず、色素包括リポソームで標識されたタンパク質を泳動させるに先だって、泳動溶液中のpH、化学種組成などがリポソーム膜へ与える影響について調べた。色素包括リポソームは、化学発光検出するにあたり、その条件下で十分に安定であることがわかった。次いで、実際に色素包括リポソームで標識したタンパク質の泳動挙動を調べ、検量線を作成し検出下限を確認した。 さらに、これらの実験の結果を踏まえて、抗体固定化ガラスビーズを用いて行う免疫分析法とキャピラリー電気泳動-化学発光検出装置との組み合わせを検討した。まず、坑-人血清アルブミンを固定化したガラスビーズを用いて免疫反応を行った(競合法)。この時、標識剤にはエオシンY包括リポソームを用いた。反応溶液をキャピラリー電気泳動-化学発光検出装置で分離・検出した。化学発光系はシュウ酸ビス(2,4,6-トリクロロフェニル)オキサレート-過酸化水素系を利用した。人血清アルブミンの検出下限は5x10^<-6>M(15nM)で、定量濃度範囲は5x10^<-6>M〜5x10^<-4>Mであった。また、本法をヒトコントロール血清中のタンパク質定量に応用した。, 日本学術振興会, 科学研究費助成事業 奨励研究(A), 1997 -1998, 奨励研究(A), 同志社大学 - Development of Separation Material for Saccharide on the Basis of Surface Imprinting Procedure
MAEDA Mizuo; TSUKAGOSHI Kazuhiko
Recognition and separation of saccharides have been the focus of much recent attention. Boronic acid is a powerful tool for this purpose since it can form a stable complex (cyclic ester) with saccharides, especially with those comprising a cis-diol group. On the other hand, microspheres from synthetic polymers have become of interest in functional materials development. In the present work, we have prepared polymer microspheres having phenylboronic acid groups on their surface and carried out their characterizations. The polymer microspheres were prepared by emulsion polymerization of stirene, butyl acrylate, and m-acrylamidophenylboronic acid. The microsphere was swollen in an alkaline aqueous suspension by adding methylethylketone so that phenylboronic acid sites in the resin would migrate out to the aqueous-organic interface in their ionized forms. D-Glucose was bound onto the microsphere while 1-methyl-alpha-D-glucoside was not, due to their different molecular structures. The adsorption of D-glucose was performed by complex (ester) formation between phenylboronic acid moiety and cis-diol group of D-glucose. Although some preliminary experiments and theoretical calculations were performed for introduction of the imprinting structure, we have not yet got the imprinted microspheres for a specific structure of saccharide with a good reproducibility. However, the data and calculations give a clue in understanding the chemical behaviors of phenylboronic acid-modified microspheres as well as the operational parameters in the preparation of saccharide-imprinted structures. The material is expected to be useful for separation and concentration of biological constituents comprising a cis-diol group, e.g., glyco-and nucleo proteins, nucleic acids as well as saccharides., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), 1995 -1996, Grant-in-Aid for Scientific Research (B), KYUSHU UNIVERSITY - Development of Novel Metal Ion Selective Ion-exchange Resin Based on Surface Template Polymerization
TAKAGI Makoto; NAKAMURA Shigeo; MAEDA Mizuo
It becomes an important research area to develop highly selective metal-chelating resin in regard to reusing metal sources. It is necessary to establish the methodology for producing materials (host or artificial receptor) that have specific ability for ion recognition. Surface imprinting is an approach to introduce special molecular recognition sites on the oil-water interface during the synthesis of ion-exchange resins. We synthesized Cu (II)-and Zn (II)-imprinted resins because these are typical metal ions which have different coordination mode. We chose oleic histamine amide as a functional monomer and divinylbenzene as a cross-linker. The imprinted resins have higher activities for absorption of metal ions but have less selectivity. For improvement of the metal ion selectivity, we stabilized the o/w emulsion during the polymerization by adding co-surfactants. In the case of adding nonion surfactant, the selectivity for Cu (II) was remarkably improved. We also prepared Cd (II)-imprinted resins that have high selectivity for Cd (II). We synthesized imprinted resins for lanthanide ions such as Eu (III) and La (III) by using surfactants containing phosphoric ester groups. In the case of using DOLPA as a functional monomer, Eu (III)-imprinted resin showed high selectivity for Eu (III). It is demonstrated the possibilty of applying the surface imprinted resins as novel materials for separating metal ions. Surface imprinting that we have been newly developed is a useful method for ion-exchange resins, because it is possible to improve the metal ion selectivities by various combination of designed functional surfactant and co-surfactant., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), 1994 -1996, Grant-in-Aid for Scientific Research (A), KYUSHU UNIVERSITY - 糖を認識する機能表面の創出とグルコースの光学分割への適用
塚越 一彦
糖に対し選択的結合能をもつ高分離機能樹脂の開発を目的として、樹脂表面官能基を鋳型ゲスト共存下に再配列させる手法(Surface Imprinting)を用い、糖鋳型を表面にもつ高分子ミクロスフェアの合成を検討した。これを用いて、グルコースの光学分割を試みた。樹脂には、申請者が開発したフェニルボロン酸基修飾ミクロスフェアを用いた。 まず、フェニルボロン酸基修飾ミクロスフェアの粒子特性、表面電荷、官能基量等に関する十分なキャラクタリゼーションを行った。本ミクロスフェアは、サブミクロン径の真球状で、表面にファニルボロン酸基に基づく負の電荷を有した。また、酸-塩基滴定およびD-グルコース飽和吸着試験から得られた表面フェニルボロン酸基量はいずれも約1×10^<-5>mol/gであった。 非鋳型樹脂およびD-グルコース鋳型樹脂へのD-グルコースの吸着量を、本研究用に改良したフェノール硫酸法に基づき評価、比較した。D-グルコース鋳型樹脂におけるD-グルコース吸着量は約80%、非鋳型樹脂におけるそれは約70%であった。すなわち、鋳型効果が確認された。しかし、L-グルコース鋳型樹脂においては鋳型効果は認められず、原因と考えられる試薬純度等について検討中である。 鋳型構造の導入には、鋳型重合時にグルコース:フェニルボロン酸基=1:2錯体の生成が必要である。すなわち、1:2錯体をいかに効率よく導入するかが今後の課題である。現在、1:2錯体の存在比を見積もる計算手法を新しく導入し、鋳型構造に関するさらなる考察を行っている。, 日本学術振興会, 科学研究費助成事業 奨励研究(A), 1995 -1995, 奨励研究(A), 同志社大学 - 機能性ミセルを用いる新規化学発光反応の開発と分析化学的応用
塚越 一彦
日本学術振興会, 科学研究費助成事業 奨励研究(A), 1992 -1992, 奨励研究(A), 九州大学 - オリゴヌクレオチド-リポソーム複合体を利用した「増幅型」核酸検出法の開発
塚越 一彦
日本学術振興会, 科学研究費助成事業 奨励研究(A), 1991 -1991, 奨励研究(A), 九州大学
Research Seeds
- Elucidation of Tube Radial Distribution Phenomenon (TRDP)Research Fields: Chemicals, Nanotechnology and MaterialsAffiliation/Name: Kazuhiko Tsukagoshi
Patent Information: 特願 2011-049138 - Development of extraction,mixing and reaction technology based on specific fluidic in a microspaceResearch Fields: Chemicals, Nanotechnology and Materials, Medical and Pharmaceutical SciencesAffiliation/Name: Kazuhiko Tsukagoshi
- Development of separation technology based on specific fluidic in a microspaceResearch Fields: Chemicals, Nanotechnology and MaterialsAffiliation/Name: Kazuhiko Tsukagoshi
Patent Information: 特願 2011-049138