| KITAGISHI Hiroaki |  |
| Faculty of Science and Engineering Department of Molecular Chemistry and Biochemistry | |
| Professor |
Last Updated :2024/12/04
Researcher Profile and Settings
researchmap
Research funding number
60448090Research Interests
Research Areas
Research Experience
- Doshisha University, Faculty of Science and Engineering, Department of Molecular Chemistry and Biochemistry, 2020/04 - Today
- Doshisha University, Faculty of Science and Engineering, Department of Molecular Chemistry and Biochemistry, 2014/04 - 2020/03
- Doshisha University, Faculty of Science and Engineering, Department of Molecular Chemistry and Biochemistry, 2008/04 - 2014/03
- Osaka University, Graduate School of Engineering Division of Applied Chemistry, Postdoctral Fellow, 2006/04 - 2008/03
Education
- Doshisha University, 工学研究科, 工業化学専攻, - 2006
- Nara Institute of Science and Technology, Graduate School of Materials Science, Department of Materials Science, - 2003
- Doshisha University, 工学部, 機能分子工学科, - 2001
Association Memberships
Awards
Published Papers
- Green-Light-Triggered and Self-Calibrated Cascade Release of Nitric Oxide and Carbon Monoxide for Synergistic Glaucoma Therapy
Xiaohua Chen; Jiamin Liu; Ming Chen; Jie Zhou; Yuyang Zhang; Xinru Hu; Weixi Geng; Qiyue Mao; Hiroaki Kitagishi; Jinquan Chen; Xuhong Qian; Youjun Yang; Yuan Lei; Xiao Luo
Journal of the American Chemical Society, 06 Nov. 2024, Scientific journal - The strategy of using heme proteins and synthetic porphyrins as injectable antidotes for fire gas poisoning
Qiyue Mao; Atsuki Nakagami; Masahito Kodera; Hiroaki Kitagishi
Chemistry Letters, 05 Nov. 2024, Scientific journal - Detoxification of hydrogen sulfide by synthetic heme-model compounds
Atsuki Nakagami; Qiyue Mao; Masaki Horitani; Masahito Kodera; Hiroaki Kitagishi
10 Jul. 2024 - An oral carbon monoxide–releasing molecule protects against acute hyperhemolysis in sickle cell disease
Kim Anh Nguyen; Alessandro Matte; Roberta Foresti; Enrica Federti; Laurent Kiger; Cécile Lefebvre; Hakim Hocini; Yanis Pelinski; Hiroaki Kitagishi; Laura Bencheikh; France Pirenne; Lucia de Franceschi; Roberto Motterlini; Pablo Bartolucci
Blood, 13 Jun. 2024, Scientific journal - Cyclodextrin‐Based Catenanes Platformed with meso‐Arylporphyrin
Kaoru Asai; Qiyue Mao; Atsuki Nakagami; Hiroaki Kitagishi
European Journal of Organic Chemistry, 10 Jun. 2024, Scientific journal - Clickable bisreactive small gold nanoclusters for preparing multifunctionalized nanomaterials: application to photouncaging of an anticancer molecule
Kenji Watanabe; Qiyue Mao; Zhouen Zhang; Machi Hata; Masahito Kodera; Hiroaki Kitagishi; Takashi Niwa; Takamitsu Hosoya
Chemical Science, 2024, Scientific journal - Inclusion Complex Formation with Tetra‐PEGylated Tetraphenylporphyrin and Face‐to‐Face Cyclodextrin Dimer through Unprecedented Molecular Threading
Qiyue Mao; Hiroaki Kitagishi
Chemistry – A European Journal, 07 Jun. 2023, Scientific journal - Development of carbon monoxide-releasing molecules conjugated to polysaccharides (glyco-CORMs) for delivering CO during obesity.
Shruti Mohan; Louis-Antoine Barel; Djamal Eddine Benrahla; Bernard Do; Qiyue Mao; Hiroaki Kitagishi; Michael Rivard; Roberto Motterlini; Roberta Foresti
Pharmacological research, 191 106770 - 106770, May 2023, Scientific journal - A synthetic porphyrin as an effective dual antidote against carbon monoxide and cyanide poisoning
Qiyue Mao; Xuansu Zhao; Akiko Kiriyama; Shigeru Negi; Yasutaka Fukuda; Hideki Yoshioka; Akira T. Kawaguchi; Roberto Motterlini; Roberta Foresti; Hiroaki Kitagishi
Proceedings of the National Academy of Sciences, 120(9 2209924120) 1 - 10, 28 Feb. 2023, Scientific journal - Spontaneous reduction of iron(iii)porphyrin to iron(ii)porphyrin–CO complex in mouse circulation
Masataka Noguchi; Qiyue Mao; Atsuki Nakagami; Hiroaki Kitagishi
Chemical Communications, 2023, Scientific journal - Effect of charged-cluster distribution of the ZIF268 zinc finger surface on cell membrane permeability
Shigeru Negi; Mami Hamori; Yuka Kawahara-Nakagawa; Masato Mashimo; Miki Imanishi; Nozomi Kuki; Yuri Kawahito; Takayuki Manabe; Nobuyuki Kawamura; Jyunichiro Yasukawa; Hiroaki Kitagishi; Yukio Sugiura
Chemistry Letters, The Chemical Society of Japan, 52(2) 75 - 78, 24 Dec. 2022, Scientific journal - Supramolecular Dual Polypeptides Induced Tubulin Aggregation for Synergistic Cancer Theranostics
Mian Tang; Yao-Hua Liu; Hua Liu; Qiyue Mao; Qilin Yu; Hiroaki Kitagishi; Ying-Ming Zhang; Lehui Xiao; Yu Liu
Journal of Medicinal Chemistry, 13 Oct. 2022, Scientific journal - Preparation, Characterization, and In Vitro Evaluation of Inclusion Complexes Formed between S-Allylcysteine and Cyclodextrins
Rino Tachikawa; Hiroki Saito; Hajime Moteki; Mitsutoshi Kimura; Hiroaki Kitagishi; Florencio Arce, Jr.; Gerard Lee See; Takashi Tanikawa; Yutaka Inoue
ACS Omega, 06 Sep. 2022, Scientific journal - Transpeptidation Reaction Mediated by Ligand- and Metal Cofactor-Substituted Sortase A from Staphylococcus aureus
Shigeru Negi; Mami Hamori; Ayaka Sato; Kyoko Shimizu; Yuka Kawahara-Nakagawa; Takayuki Manabe; Nobuhito Shibata; Hiroaki Kitagishi; Masato Mashimo; Yukio Sugiura
Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, 95(7) 1025 - 1031, 15 Jul. 2022, Scientific journal - Involvement of trace metals in the reduction of disulfide bonds by glutathione
Shigeru Negi; Mami Hamori; Rina Hashimoto; Yuka Nakagawa-Kawahara; Hiroaki Kitagishi; Yukio Sugiura
Harris Science Research Institute of Doshisha University, 63(1) 45 - 49, 30 Apr. 2022 - Capture of carbon monoxide using a heme protein model: from biomimetic chemistry of heme proteins to physiological and therapeutic applications
Hiroaki Kitagishi; Qiyue Mao
Polymer Journal, Apr. 2022, Scientific journal - Importance of two-dimensional cation clusters induced by protein folding in intrinsic intracellular membrane permeability
Shigeru Negi; Mami Hamori; Yuka Kawahara-Nakagawa; Miki Imanishi; Miku Kurehara; Chieri Kitada; Yuri Kawahito; Kanae Kishi; Takayuki Manabe; Nobuyuki Kawamura; Hiroaki Kitagishi; Masato Mashimo; Nobuhito Shibata; Yukio Sugiura
RSC Chemical Biology, Royal Society of Chemistry (RSC), 3(8) 1076 - 1084, 2022, Scientific journal - Sensitive quantification of carbon monoxide in vivo reveals a protective role of circulating hemoglobin in CO intoxication
Qiyue Mao; Akira T. Kawaguchi; Shun Mizobata; Roberto Motterlini; Roberta Foresti; Hiroaki Kitagishi
Communications Biology, 4(1), Dec. 2021, Scientific journal - FRET‐Based In‐Cell Detection of Highly Selective Supramolecular Complexes of meso‐Tetraarylporphyrin with Peptide/BODIPY‐Modified Per‐O‐Methyl‐β‐Cyclodextrins
Atsuki Nakagami; Qiyue Mao; Géraldine Gouhier; Hidetoshi Arima; Hiroaki Kitagishi
ChemBioChem, Wiley, 16 Nov. 2021, Scientific journal - Synthetic heme protein models that function in aqueous solution
Hiroaki Kitagishi; Koji Kano
Chemical Communications, 57(2) 148 - 173, 07 Jan. 2021, Scientific journal - Functional Myoglobin Model Composed of a Strapped Porphyrin/Cyclodextrin Supramolecular Complex with an Overhanging COOH That Increases O
2 /CO Binding Selectivity in Aqueous Solution
Qiyue Mao; Pradip K. Das; Stéphane Le Gac; Bernard Boitrel; Vincent Dorcet; Koji Oohora; Takashi Hayashi; Hiroaki Kitagishi
Inorganic Chemistry, 60(16) 12392 - 12404, 2021, Scientific journal - Intracellular Delivery of Adamantane-Tagged Small Molecule, Proteins, and Liposomes Using an Octaarginine-Conjugated β-Cyclodextrin
Hiroaki Kitagishi; Misa Jiromaru; Naomi Hasegawa
ACS Applied Bio Materials, 3(8) 4902 - 4911, 17 Aug. 2020, Scientific journal - Carbon monoxide, a retrograde messenger generated in postsynaptic mushroom body neurons, evokes noncanonical dopamine release
Kohei Ueno; Johannes Morstein; Kyoko Ofusa; Shintaro Naganos; Ema Suzuki-Sawano; Saika Minegishi; Samir P. Rezgui; Hiroaki Kitagishi; Brian W. Michel; Christopher J. Chang; Junjiro Horiuchi; Minoru Saitoe
Journal of Neuroscience, 40(18) 3533 - 3548, Apr. 2020, Scientific journal - Boronate sol-gel method for one-step fabrication of polyvinyl alcohol hydrogel coatings by simple cast- And dip-coating techniques
Ryuhei Nishiyabu; Yuki Takahashi; Taro Yabuki; Shoji Gommori; Yuki Yamamoto; Hiroaki Kitagishi; Yuji Kubo
RSC Advances, 10(1) 86 - 94, 01 Jan. 2020, Scientific journal - Supramolecular Complexation in Biological Media: NMR Study on Inclusion of an Anionic Tetraarylporphyrin into a Per‐ O ‐Methylated β‐Cyclodextrin Cavity in Serum, Blood, and Urine
Hiroaki Kitagishi; Mai Saito; Qiyue Mao; Akiko Kiriyama; Shigeru Negi; Koji Kano
Chemistry – An Asian Journal, Wiley, 14(19) 3320 - 3328, Oct. 2019, Scientific journal - Design and Biological Evaluation of Manganese‐ and Ruthenium‐Based Hybrid CO‐RMs (HYCOs)
Anthony Ollivier; Roberta Foresti; Zeina El Ali; Thierry Martens; Hiroaki Kitagishi; Roberto Motterlini; Michael Rivard
ChemMedChem, Wiley, 14(18) 1684 - 1691, Sep. 2019, Scientific journal - Modelling haemoproteins: porphyrins and cyclodextrins as sources of inspiration
Maxime Vonesch; Jennifer A. Wytko; Hiroaki Kitagishi; Koji Kano; Jean Weiss
Chemical Communications, 55(97) 14558 - 14565, 2019, Scientific journal - Preparation, characterization, and study of the antimicrobial activity of a Hinokitiol-copper(II)/γ-cyclodextrin ternary complex
Suzuki R; Inoue Y; Murata I; Nomura H; Isshiki Y; Hashimoto M; Kudo Y; Kitagishi H; Kondo S; Kanamoto I
Journal of Molecular Structure, 1194 19 - 27, 2019, Scientific journal - Hydrogen bond directed molecular recognition in water in a strapped-porphyrin-cyclodextrin assembly
Kitagishi H; Ohara K; Shimoji D; Vonesch M; Weiss J; Wytko J.A
Journal of Porphyrins and Phthalocyanines, 23(4-5) 569 - 574, 2019, In book - Arginine residues provide a multivalent effect for cellular uptake of a hemoprotein assembly
Oohora K; Kajihara R; Jiromaru M; Kitagishi H; Hayashi T
Chemistry Letters, 48(3) 295 - 298, 2019, Scientific journal - Acceleration of hydrolytic DNA cleavage by dicopper(II) complexes with p-cresol-derived dinucleating ligands at slightly acidic pH and mechanistic insights
Kodera M; Kadoya Y; Aso K; Fukui K; Nomura A; Hitomi Y; Kitagishi H
Bulletin of the Chemical Society of Japan, 92(4) 739 - 747, 2019, Scientific journal - Circadian clock disruption by selective removal of endogenous carbon monoxide
Saika Minegishi; Ikuko Sagami; Shigeru Negi; Koji Kano; Hiroaki Kitagishi
Scientific Reports, Springer Nature, 8(1), Dec. 2018, Scientific journal - PEGylated carboxyhemoglobin bovine (SANGUINATE) ameliorates myocardial infarction in a rat model
Akira T. Kawaguchi; Amankeldi A. Salybekov; Mariko Yamano; Hiroaki Kitagishi; Kaori Sekine; Tetsuro Tamaki
Artificial Organs, 42(12) 1174 - 1184, Dec. 2018, Scientific journal - Carbon monoxide-induced metabolic switch in adipocytes improves insulin resistance in obese mice
Laura Braud; Maria Pini; Lucie Muchova; Sylvie Manin; Hiroaki Kitagishi; Daigo Sawaki; Gabor Czibik; Julien Ternacle; Geneviève Derumeaux; Roberta Foresti; Roberto Motterlini
JCI insight, 3(22), 15 Nov. 2018, Scientific journal - Optimized synthesis of a per-O-methylated β-cyclodextrin dimer linked at the secondary face by a pyridine ligand
Qiyue Mao; Hiroaki Kitagishi
Journal of Inclusion Phenomena and Macrocyclic Chemistry, Springer Science and Business Media {LLC}, 93(1-2) 67 - 76, Sep. 2018, Scientific journal - A water-soluble supramolecular complex that mimics the heme/copper hetero-binuclear site of cytochrome: C oxidase
Hiroaki Kitagishi; Daiki Shimoji; Takehiro Ohta; Ryo Kamiya; Yasuhiro Kudo; Akira Onoda; Takashi Hayashi; Jean Weiss; Jennifer A. Wytko; Koji Kano
Chemical Science, Royal Society of Chemistry, 9(7) 1989 - 1995, 21 Feb. 2018, Scientific journal - A water-soluble supramolecular complex that mimics the heme/copper hetero-binuclear site of cytochrome: C oxidase
Hiroaki Kitagishi; Daiki Shimoji; Takehiro Ohta; Ryo Kamiya; Yasuhiro Kudo; Akira Onoda; Takashi Hayashi; Jean Weiss; Jennifer A. Wytko; Koji Kano
Chemical Science, Royal Society of Chemistry, 9(7) 1989 - 1995, 21 Feb. 2018, Scientific journal - Iron(II)porphyrin Cyclodextrin Supramolecular Complex as a Carbon Monoxide-Depleting Agent in Living Organisms
Hiroaki Kitagishi; Saika Minegishi
CHEMICAL & PHARMACEUTICAL BULLETIN, PHARMACEUTICAL SOC JAPAN, 65(4) 336 - 340, Apr. 2017 - Detection and Removal of Endogenous Carbon Monoxide by Selective and Cell-Permeable Hemoprotein Model Complexes
Saika Minegishi; Aki Yumura; Hirotsuna Miyoshi; Shigeru Negi; Shigeru Taketani; Roberto Motterlini; Roberta Foresti; Koji Kano; Hiroaki Kitagishi
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 139(16) 5984 - 5991, Apr. 2017, Scientific journal - Participation of Hydroxyl Radical in the Formation of Verdoheme-type Compound in the Reaction of a Ferric Porphyrin with Hydrogen Peroxide
Hiroaki Kitagishi; Yosuke Sugaya; Kozue Komazawa; Mariko Tamaki; Koji Kano
CHEMISTRY LETTERS, CHEMICAL SOC JAPAN, 46(4) 509 - 512, Apr. 2017, Scientific journal - HemoCD as a Totally Synthetic Artificial Oxygen Carrier: Improvements in the Synthesis and O-2/CO Discrimination
Hiroaki Kitagishi; Qiyue Mao; Naoya Kitamura; Takahiro Kita
ARTIFICIAL ORGANS, WILEY, 41(4) 372 - 380, Apr. 2017, Scientific journal - Surface modification of a polyvinyl alcohol sponge with functionalized boronic acids to develop porous materials for multicolor emission, chemical sensing and 3D cell culture
Ryuhei Nishiyabu; Shunsuke Iizuka; Saika Minegishi; Hiroaki Kitagishi; Yuji Kubo
CHEMICAL COMMUNICATIONS, ROYAL SOC CHEMISTRY, 53(25) 3563 - 3566, Mar. 2017, Scientific journal - Intramolecular Oxidative O-Demethylation of an Oxoferryl Porphyrin Complexed with a Per-O-methylated beta-Cyclodextrin Dimer
Hiroaki Kitagishi; Shun Kurosawa; Koji Kano
Chemistry-An Asian Journal, WILEY-V C H VERLAG GMBH, 11(22) 3213 - 3219, Nov. 2016, Scientific journal - Esterification of 24S-OHC induces formation of atypical lipid droplet-like structures, leading to neuronal cell death
Wakako Takabe; Yasuomi Urano; Diep-Khanh Ho Vo; Kimiyuki Shibuya; Masaki Tanno; Hiroaki Kitagishi; Toyoshi Fujimoto; Noriko Noguchi
JOURNAL OF LIPID RESEARCH, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 57(11) 2005 - 2014, Nov. 2016, Scientific journal - Synthesis of 24(S)-hydroxycholesterol esters responsible for the induction of neuronal cell death
Kimiyuki Shibuya; Toshiaki Watanabe; Yasuomi Urano; Wakako Takabe; Noriko Noguchi; Hiroaki Kitagishi
BIOORGANIC & MEDICINAL CHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, 24(11) 2559 - 2566, Jun. 2016, Scientific journal - Feedback Response to Selective Depletion of Endogenous Carbon Monoxide in the Blood
Hiroaki Kitagishi; Saika Minegishi; Aki Yumura; Shigeru Negi; Shigeru Taketani; Yoko Amagase; Yumiko Mizukawa; Tetsuro Urushidani; Yukio Sugiura; Koji Kano
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 138(16) 5417 - 5425, Apr. 2016, Scientific journal - Photoinduced Homolysis of Alkyl-Cobalt(III) Bonds in a Cyclodextrin Cage
Kohei Imabeppu; Hiroyuki Kuwano; Eriko Yutani; Hiroaki Kitagishi; Koji Kano
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, WILEY-V C H VERLAG GMBH, 2016(12) 1784 - 1789, Apr. 2016, Scientific journal - Bioconjugation of Serum Albumin to a Maleimide-appended Porphyrin/Cyclodextrin Supramolecular Complex as an Artificial Oxygen Carrier in the Bloodstream
Hiroaki Kitagishi; Hiroki Kawasaki; Koji Kano
CHEMISTRY-AN ASIAN JOURNAL, WILEY-V C H VERLAG GMBH, 10(8) 1768 - 1775, Aug. 2015, Scientific journal - Reaction between a haemoglobin model compound and hydrosulphide in aqueous solution
Kenji Watanabe; Toshikane Suzuki; Hiroaki Kitagishi; Koji Kano
CHEMICAL COMMUNICATIONS, ROYAL SOC CHEMISTRY, 51(19) 4059 - 4061, 2015, Scientific journal - Supramolecular intracellular delivery of an anionic porphyrin by octaarginine-conjugated per-O-methyl-beta-cyclodextrin
Hiroaki Kitagishi; Fumihiko Chai; Shigeru Negi; Yukio Sugiura; Koji Kano
CHEMICAL COMMUNICATIONS, ROYAL SOC CHEMISTRY, 51(12) 2421 - 2424, 2015, Scientific journal - Intracellular Delivery of Anionic meso-Tetraarylporphyrin-Per-O-methylated beta-Cyclodextrin Supramolecular Complexes by an Oligoarginine Carrier Peptide
Hiroaki Kitagishi; Keiko Minami; Koji Kano
CHEMISTRY LETTERS, CHEMICAL SOC JAPAN, 43(7) 1095 - 1097, Jul. 2014, Scientific journal - Supramolecular ferric porphyrins and a cyclodextrin dimer as antidotes for cyanide poisoning
T. Yamagiwa; A. T. Kawaguchi; T. Saito; S. Inoue; S. Morita; K. Watanabe; H. Kitagishi; K. Koji; S. Inokuchi
HUMAN & EXPERIMENTAL TOXICOLOGY, SAGE PUBLICATIONS LTD, 33(4) 360 - 368, Apr. 2014, Scientific journal - Hydroxylation of an axial pyridine ligand in a supramolecule composed of a porphinatoiron(III) and a per-O-methyl-beta-cyclodextrin dimer with mCPBA
T. Ueda; S. Kumeda; H. Kitagishi; K. Kano
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, SPRINGER, 19 S546 - S546, Mar. 2014 - Intramolecular Direct Oxygen Transfer from Oxoferryl Porphyrin to a Sulfide Bond
Takunori Ueda; Hiroaki Kitagishi; Koji Kano
INORGANIC CHEMISTRY, AMER CHEMICAL SOC, 53(1) 543 - 551, Jan. 2014, Scientific journal - Intramolecular Oxidative O-Demethylation in a Per-O-methylated beta-Cyclodextrin-Iron Porphyrin Inclusion Complex in Aqueous Solution
Takunori Ueda; Saki Kumeda; Hiroaki Kitagishi; Koji Kano
CHEMISTRY LETTERS, CHEMICAL SOC JAPAN, 42(11) 1366 - 1368, Nov. 2013, Scientific journal - Elongation of Circulation Time of a Diatomic Molecule Receptor in Bloodstream by Attachment to Small Gold Nanoparticles
Keiichi Karasugi; Hiroaki Kitagishi; Koji Kano
CHEMISTRY LETTERS, CHEMICAL SOC JAPAN, 42(2) 115 - 117, Feb. 2013, Scientific journal - Supramolecular Iron Porphyrin/Cyclodextrin Dimer Complex that Mimics the Functions of Hemoglobin and Methemoglobin
Kenji Watanabe; Hiroaki Kitagishi; Koji Kano
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, WILEY-V C H VERLAG GMBH, 52(27) 6894 - 6897, 2013, Scientific journal - Supramolecular Linear Assemblies of Cytochrome b(562) Immobilized on a Gold Electrode
Yasuaki Kakikura; Akira Onoda; Emi Kubo; Hiroaki Kitagishi; Taro Uematsu; Susumu Kuwabata; Takashi Hayashi
JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS, SPRINGER, 23(1) 172 - 179, Jan. 2013, Scientific journal - Cellular uptake of octaarginine-conjugated tetraarylporphyrin included by per-O-methylated beta-cyclodextrin
Hiroaki Kitagishi; Satoshi Hatada; Toshiaki Itakura; Yuki Maki; Yasuaki Maeda; Koji Kano
ORGANIC & BIOMOLECULAR CHEMISTRY, ROYAL SOC CHEMISTRY, 11(19) 3203 - 3211, 2013, Scientific journal - Modification of a Dioxygen Carrier, HemoCD, with PEGylated Dendrons for Extension of Circulation Time in the Bloodstream
Keiichi Karasugi; Hiroaki Kitagishi; Koji Kano
BIOCONJUGATE CHEMISTRY, AMER CHEMICAL SOC, 23(12) 2365 - 2376, Dec. 2012, Scientific journal - Glycan-Targeted Virus-like Nanoparticles for Photodynamic Therapy
Jin-Kyu Rhee; Michael Baksh; Corwin Nycholat; James C. Paulson; Hiroaki Kitagishi; M. G. Finn
BIOMACROMOLECULES, AMER CHEMICAL SOC, 13(8) 2333 - 2338, Aug. 2012, Scientific journal - Functionalization of a Protein Surface with Per-O-Methylated beta-Cyclodextrin
Hiroaki Kitagishi; Kazuya Kashiwa; Koji Kano
BIOPOLYMERS, WILEY-BLACKWELL, 97(1) 11 - 20, Jan. 2012, Scientific journal - Supramolecular dioxygen receptors composed of an anionic water-soluble porphinatoiron(II) and cyclodextrin dimers
Koji Kano; Syoichi Chimoto; Mariko Tamaki; Yoshiki Itoh; Hiroaki Kitagishi
DALTON TRANSACTIONS, ROYAL SOC CHEMISTRY, 41(2) 453 - 461, 2012, Scientific journal - PEGylation of an artificial O-2 and CO receptor: synthesis, characterisation and pharmacokinetic study
Takunori Ueda; Hiroaki Kitagishi; Koji Kano
ORGANIC & BIOMOLECULAR CHEMISTRY, ROYAL SOC CHEMISTRY, 10(22) 4337 - 4347, 2012, Scientific journal - Supramolecular Ferric Porphyrins as Cyanide Receptors in Aqueous Solution
Kenji Watanabe; Hiroaki Kitagishi; Koji Kano
ACS MEDICINAL CHEMISTRY LETTERS, AMER CHEMICAL SOC, 2(12) 943 - 947, Dec. 2011, Scientific journal - Preparation and Function of Poly(acrylic acid)s Modified by Supramolecular Complex Composed of Porphinatoiron and a Cyclodextrin Dimer That Bind Diatomic Molecules (O-2 and CO) in Aqueous Solution
Koji Kano; Toshiro Ochi; Sayuri Okunaka; Yusuke Ota; Keiichi Karasugi; Takunori Ueda; Hiroaki Kitagishi
CHEMISTRY-AN ASIAN JOURNAL, WILEY-BLACKWELL, 6(11) 2946 - 2955, Nov. 2011, Scientific journal - Colorful Virus-like Particles: Fluorescent Protein Packaging by the Q beta Capsid
Jin-Kyu Rhee; Marisa Hovlid; Jason D. Fiedler; Steven D. Brown; Florian Manzenrieder; Hiroaki Kitagishi; Corwin Nycholat; James C. Paulson; M. G. Finn
BIOMACROMOLECULES, AMER CHEMICAL SOC, 12(11) 3977 - 3981, Nov. 2011, Scientific journal - Calorimetric Study on Coordination of Tridentate Imidazolyl Calix[6]arene Ligands to Zinc Ion in Organic Solvents
Koji Kano; Masataka Kondo; Haruka Inoue; Hiroaki Kitagishi; Benoit Colasson; Olivia Reinaud
INORGANIC CHEMISTRY, AMER CHEMICAL SOC, 50(13) 6353 - 6360, Jul. 2011, Scientific journal - Preparation and reactivity of a tetranuclear Fe(II) core in the metallothionein alpha-domain
Yohei Sano; Akira Onoda; Rie Sakurai; Hiroaki Kitagishi; Takashi Hayashi
JOURNAL OF INORGANIC BIOCHEMISTRY, ELSEVIER SCIENCE INC, 105(5) 702 - 708, May 2011, Scientific journal - Gold Nanoparticles Carrying Diatomic Molecules (O-2 and CO) in Aqueous Solution
Keiichi Karasugi; Hiroaki Kitagishi; Koji Kano
CHEMISTRY-AN ASIAN JOURNAL, WILEY-BLACKWELL, 6(3) 825 - 833, Mar. 2011, Scientific journal - A chemically-controlled supramolecular protein polymer formed by a myoglobin-based self-assembly system
Koji Oohora; Akira Onoda; Hiroaki Kitagishi; Hiroyasu Yamaguchi; Akira Harada; Takashi Hayashi
CHEMICAL SCIENCE, ROYAL SOC CHEMISTRY, 2(6) 1033 - 1038, 2011, Scientific journal - Oxoferryl Porphyrin/Hydrogen Peroxide System Whose Behavior is Equivalent to Hydroperoxoferric Porphyrin
Hiroaki Kitagishi; Mariko Tamaki; Takunori Ueda; Shun Hirota; Takehiro Ohta; Yoshinori Naruta; Koji Kano
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 132(47) 16730 - 16732, Dec. 2010, Scientific journal - Structural Basis of the Lactate-dependent Allosteric Regulation of Oxygen Binding in Arthropod Hemocyanin
Shun Hirota; Naoki Tanaka; Ivan Micetic; Paolo Di Muro; Satoshi Nagao; Hiroaki Kitagishi; Koji Kano; Richard S. Magliozzo; Jack Peisach; Mariano Beltramini; Luigi Bubacco
JOURNAL OF BIOLOGICAL CHEMISTRY, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 285(25) 19338 - 19345, Jun. 2010, Scientific journal - A Diatomic Molecule Receptor That Removes CO in a Living Organism
Hiroaki Kitagishi; Shigeru Negi; Akiko Kiriyama; Akino Honbo; Yukio Sugiura; Akira T. Kawaguchi; Koji Kano
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, WILEY-V C H VERLAG GMBH, 49(7) 1312 - 1315, 2010, Scientific journal - Thermodynamically Controlled Supramolecular Polymerization of Cytochrome b(562)
Hiroaki Kitagishi; Koji Oohora; Takashi Hayashi
BIOPOLYMERS, WILEY-BLACKWELL, 91(3) 194 - 200, Mar. 2009, Scientific journal - HemoCD as an Artificial Oxygen Carrier: Oxygen Binding and Autoxidation
Koji Kano; Hiroaki Kitagishi
ARTIFICIAL ORGANS, WILEY-BLACKWELL PUBLISHING, INC, 33(2) 177 - 182, Feb. 2009, Scientific journal - Self-Assembly of One- and Two-Dimensional Hemoprotein Systems by Polymerization through Heme-Heme Pocket Interactions
Hiroaki Kitagishi; Yasuaki Kakikura; Hiroyasu Yamaguchi; Koji Oohora; Akira Harada; Takashi Hayashi
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, WILEY-V C H VERLAG GMBH, 48(7) 1271 - 1274, 2009, Scientific journal - 水中で二原子分子(O2,CO,NO)を捕捉する完全合成化合物
北岸 宏亮; 加納 航治
人工血液, 17(4) 160 - 168, 2009 - A supramolecular receptor of diatomic molecules (O-2, CO, NO) in aqueous solution
Koji Kano; Yoshiki Itoh; Hiroaki Kitagishi; Takashi Hayashi; Shun Hirota
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 130(25) 8006 - 8015, Jun. 2008, Scientific journal - Supramolecular hemoprotein linear assembly by successive interprotein heme-heme pocket interactions
Hiroaki Kitagishi; Koji Oohora; Hiroyasu Yamaguchi; Hideaki Sato; Takashi Matsuo; Akira Harada; Takashi Hayashi
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 129(34) 10326 - +, Aug. 2007, Scientific journal - Characterization of an inclusion complex of 5,10,15,20-tetrakis(4- sulfonatophenyl)-porphinato iron and an O-methylated β-cyclodextrin dimer having a pyridine linker and its related complexes in aqueous solution
Koji Kano; Hiroaki Kitagishi; Shigeki Tanaka
Journal of Inclusion Phenomena and Macrocyclic Chemistry, 56(1-2) 69 - 74, Oct. 2006, International conference proceedings - Redox behavior of a manganese porphyrin complexed with per-O-methylated beta-cyclodextrin in aqueous solution
Koji Kano; Hiroaki Kitagishi; Yumiko Sone; Nami Nakazawa; Masahito Kodera
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, WILEY-V C H VERLAG GMBH, 2006(20) 4043 - 4053, Oct. 2006, Scientific journal - Characterization of an inclusion complex of 5,10,15,20-tetrakis(4-sulfonatophenyl)-porphinato iron and an O-methylated beta-cyclodextrin dimer having a pyridine linker and its related complexes in aqueous solution
Koji Kano; Hiroaki Kitagishi; Shigeki Tanaka
JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY, SPRINGER, 56(1-2) 69 - 74, Oct. 2006, Scientific journal - A myoglobin functional model composed of a ferrous porphyrin and a cyclodextrin dimer with an imidazole linker
Koji Kano; Hiroaki Kitagishi; Takahiro Mahuchi; Masahito Kodera; Shun Hirota
CHEMISTRY-AN ASIAN JOURNAL, WILEY-V C H VERLAG GMBH, 1(3) 358 - 366, Sep. 2006, Scientific journal - Iron porphyrin-cyclodextrin supramolecular complex as a functional model of myoglobin in aqueous solution
K Kano; H Kitagishi; C Dagallier; M Kodera; T Matsuo; T Hayashi; Y Hisaeda; S Hirota
INORGANIC CHEMISTRY, AMER CHEMICAL SOC, 45(11) 4448 - 4460, May 2006, Scientific journal - Dioxygen binding to a simple myoglobin model in aqueous solution
K Kano; H Kitagishi; M Kodera; S Hirota
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, WILEY-V C H VERLAG GMBH, 44(3) 435 - 438, 2005, Scientific journal - Anion binding to a ferric porphyrin complexed with per-O-methylated beta-cyclodextrin in aqueous solution
K Kano; H Kitagishi; S Tamura; A Yamada
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 126(46) 15202 - 15210, Nov. 2004, Scientific journal - Heptakis(2,3-di-O-carboxymethyl)-beta-cyclodextrin as a pH-sensitive host
K Kano; Y Horiki; T Mabuchi; H Kitagishi
CHEMISTRY LETTERS, CHEMICAL SOC JAPAN, 33(9) 1086 - 1087, Sep. 2004, Scientific journal - Dynamic structural change of a twisted hexaporphyrin complex by highly specific molecular recognition
H Kitagishi; A Satake; Y Kobuke
INORGANIC CHEMISTRY, AMER CHEMICAL SOC, 43(11) 3394 - 3405, May 2004, Scientific journal - Simple Myoglobin Model Composed of Permethylated β-Cyclodextrin Dimmer and Water-Soluble Fe(II)porphyrin
Kano, K; Kitagishi, H; Kodera, M; Hirota, S
International Conference on Supramolecular Science and Technology, 98 s71 , 01 Apr. 2004
MISC
- 内在性一酸化炭素の選択的除去が及ぼす体内時計システムへの影響
北岸宏亮; 峯岸彩夏; 根木滋; 佐上郁子; 加納航治
人工血液, 26(1), 2018 - 生体内一酸化炭素の除去がサーカディアンリズムに及ぼす影響
北岸宏亮; 峯岸彩夏; 佐上郁子; 根木滋; 加納航治
シクロデキストリンシンポジウム講演要旨集, 35th, 2018 - マウス体内COの選択的除去によって誘発される体内時計リズム変化
峯岸彩夏; 北岸宏亮; 根木滋; 加納航治
日本化学会春季年会講演予稿集(CD-ROM), 97th, 2017 - 生体内COレセプターのin vivo投与によって誘発される体内時計遺伝子群の発現量変化
峯岸彩夏; 北岸宏亮; 根木滋; 加納航治
日本化学会春季年会講演予稿集(CD-ROM), 96th, 2016 - 血液中における内因性COの選択的除去とそれによる遺伝子発現の変化
峯岸彩夏; 勇村亞希; 北岸宏亮; 根木滋; 杉浦幸雄; 加納航治
日本化学会講演予稿集, 95th(4), 2015 - Genetic response upon removal of endogenous CO from the blood of mice by administration of a synthetic CO receptor
H. Kitagishi; A. Yumura; K. Kano; S. Negi; Y. Mizukawa; Y. Amagase; T. Urushidani; Y. Sugiura
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, SPRINGER, 19 S394 - S394, Mar. 2014, Summary international conference - 超分子COレセプターによる生体内COの除去効果
勇村亞希; 北岸宏亮; 根木滋; 杉浦幸雄; 加納航治
日本化学会講演予稿集, 94th(3), 2014 - 超分子ヘムタンパク質モデル化合物による生体内COの除去
北岸宏亮; 根木滋; 勇村亞希; 杉浦幸雄; 加納航治
生体分子科学討論会講演要旨集, 41st, 2014 - 生体内一酸化炭素の選択的除去による生理機能探索
峯岸彩夏; 勇村亞希; 北岸宏亮; 根木滋; 杉浦幸雄; 加納航治
シクロデキストリンシンポジウム講演要旨集, 31st, 2014 - Application of Supramolecular Hemoprotein Model Compounds Toward Biological Systems
北岸 宏亮; 加納 航治
同志社大学理工学研究報告, 同志社大学理工学研究所, 54(4) 71 - 74, Jan. 2014 - シクロデキストリン-ポルフィリン超分子錯体による転写因子GAL4のDNA結合の可逆的制御
根木滋; 家出悠加; 中山千絵; 北岸宏亮; 加納航治; 杉浦幸雄
シクロデキストリンシンポジウム講演要旨集, 30th, 2013 - Artificial Cyanide Ion and Dioxygen Receptors
渡辺 賢司; 北岸 宏亮; 加納 航治
同志社大学理工学研究報告, 同志社大学理工学研究所, 53(4) 45 - 49, Jan. 2013 - Cell-targeted photodynamic therapy using virus-like particles
Jin-Kyu Rhee; Michael M. Baksh; Hiroaki Kitagishi; Corwin Nycholat; James C. Paulson; M. G. Finn
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 243, Mar. 2012, Summary international conference - IMIDAZOLE CYCLODEXTRIN (IMCD), TOTALLY SYNTHETIC SUPRAMOLECULAR COMPLEX AS AN ANTIDOTE FOR CYANIDE POISONING
T. Yamagiwa; A. T. Kawaguchi; T. Saito; K. Umezawa; H. Kitagishi; K. Watanabe; K. Kano; S. Inokuchi
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, WICHTIG EDITORE, 34(8) 621 - 621, Aug. 2011, Summary international conference - Thermodynamic investigation to DNA condensation mechanism by oligoarginine
谷口仁哉; 根木滋; 北岸宏亮; 加納航治; 杉浦幸雄
日本化学会講演予稿集, 89th(2), 2009 - Artificial Supramolecular System That Functions in Vivo: Capture and Removal of Endogenous CO by an Iron(II)Porphyrin-Cyclodextrin Inclusion Complex
北岸宏亮; 根木滋; 喜里山暁子; 本保亜希乃; 杉浦幸雄; 加納航治
日本化学会講演予稿集, 89th(2), 2009 - 鉄(II)ポルフィリン錯体の尿中排泄挙動とCO捕捉効果
本保亜希乃; 喜里山暁子; 根木滋; 北岸宏亮; 杉浦幸雄; 加納航治
日本薬学会年会要旨集, 129th(4), 2009 - 多価アニオン性ポルフィリンの自己会合がHIV-1のV3 loopとの相互作用に及ぼす影響
渡辺賢司; 根木滋; 北岸宏亮; 杉浦幸雄; 加納航治
日本化学会バイオテクノロジー部会シンポジウム講演要旨集, 12th, 2009 - Completely Synthetic Compounds That Bind Diatomic Molecules (O2,CO,NO) in Aqueous Solution
H. Kitagishi; K. Kano
17(4) 160 - 168, 2009 - COLL 122-Cyclodextrin dimers as simple myoglobin models in aqueous solution
Koji Kano; Hiroaki Kitagishi
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, 236, Aug. 2008, Summary international conference
Research Projects
- Study on Biologically Active Gas Molecules Using Synthetic Heme-model Compounds
北岸 宏亮; 當舎 武彦; 小寺 政人
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2024/04 -2027/03, Grant-in-Aid for Scientific Research (B), Doshisha University - Medical role of carbon monoxide - Effect on superficial wound healing -
川口 玄; 北岸 宏亮; 山田 佳之
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2024/04 -2027/03, Grant-in-Aid for Scientific Research (C), Tokai University - 細胞内小器官を標的してがん細胞選択的な細胞死を誘導する金属錯体の開発
小寺 政人; 北岸 宏亮
日本学術振興会, 科学研究費助成事業, 2022/04 -2025/03, 基盤研究(B), 同志社大学 - Mechanistic study on circadian clock system regulated by heme/CO crosstalk
北岸 宏亮; 廣田 毅
本研究では細胞内概日リズム制御におけるヘムと一酸化炭素のクロストーク性を解明することを目的としており，2021年度にはそれを可能とする化合物群の合成と，細胞内一酸化炭素を計測するための独自のアッセイを確立して報告した。まず細胞内一酸化炭素を捕捉するためのケミカルツールhemoCDを用いたアッセイを確立した。動物体内の細胞には微量の内因性一酸化炭素(CO)が常時存在するが，それを簡便かつ正確に計測する方法はこれまでに報告されていなかった。我々は細胞内や動物組織内に含まれるCOをhemoCDを使って簡便かつ正確に計測することを試み成功した。この定量方法により，デキサメタゾンで同期化した細胞内に含まれるCOの産生には概日リズムが存在することが明らかとなった。さらに動物を用いて組織内に含まれるCOの定量方法を確立した。この手法は，内在性COのシグナル伝達物質としての機能解明に役立つだけでなく，過剰のCOを吸入した体内における毒性メカニズム解明にも役立てることができ，論文公表後にプレスリリースにより広く周知した。その結果種々の共同研究および知財としての権利化を遂行することとなった。さらに概日リズム解明のためのケミカルツールとして，細胞内でCOを効率よく放出する新規CO-releasing moleculeの開発，ヘムオキシゲナーゼによって分解されてもCOを発生しないメソメチルヘム誘導体の合成にも成功しており，次年度からの研究に貢献するケミカルツールが種々揃っている段階である。, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), 2020/04 -2023/03, Grant-in-Aid for Scientific Research (B), Doshisha University - Development of innovative drugs having multistep action for treatment of central cerebral amyloidosis
有馬 英俊; 川上 茂; 北岸 宏亮; 山吉 麻子; 斉藤 貴志
アルツハイマー病に対する薬剤の臨床試験は数多くなされているが、その成功例はほとんどなく、有効な治療薬の開発が強く望まれている。我々はアルツハイマー病に対する複数の危険因子に対して個々に作用する薬剤ではなく、5つの危険因子（脳におけるAPOε4遺伝子発現、コレステロール蓄積、脳炎症反応、アミロイド線維形成・蓄積、神経原繊維変化）に対して多段階かつ同時に抑制する薬剤の開発を期待して、我々が開発した新規化合物であるシクロデキストリン/ポリアミドアミンデンドリマー結合体（CDE)に、さらに静脈内投与後の有効性を可能にする血液脳関門（BBB）透過リガンドを結合したBPB-CDEの構築に関する研究を行っている。また、Apoε4遺伝子発現を特異的に抑制できるsiRNA（Apo-siRNA）とBPB-CDEとの複合体を調製し、それらの化学的・物理的性質、細胞膜透過性、Aβ凝集・繊維形成抑制・溶解効果などの検討を予定し、2021年度は次のような検討を行った。β-シクロデキストリンとPAMAMデンドリマーとの結合体（CDE）にBBB透過リガンドを結合体したBPP-CDEの合成・精製・構造解析を行った。Aβペプチド（1-40）及びAβペプチド（1-42）の凝集体形成に及ぼすPAMAMデンドリマー及びBPP-CDEの影響について検討を行った。また、Aβペプチド（1-40）とPAMAMデンドリマー及びBPP-CDE との相互作用をNMRを用いて検討した。PAMAMデンドリマー及びBPP-CDEとsiRNA複合体を調製し、その粒子径・PDI・ゼータ電位等の物性について測定する準備を行った。論文公表１件、学会発表２件を行った。, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), 2020/04 -2023/03, Grant-in-Aid for Scientific Research (B), Daiichi University, College of Pharmaceutical Sciences - Development of non-viral vector to realize treatment of hereditary disease using genome editing RNA technology
有馬 英俊; 城野 博史; 北岸 宏亮
本研究では、肝臓の遺伝子疾患である家族性アミロイドポリニューロパチー（FAP）に対する根治療法としてのin vivoゲノム編集技術の開発を目的に、(1) 肝実質細胞特異的非ウイルスベクターであるデンドリマー/シクロデキストリン結合体（CDE）にラクトースまたはN-アセチルガラクトサミンおよびポリエチレングリコ―ル（PEG）を結合したそれぞれL-PaCおよびGN-PaCの構築、(2) ゲノム編集効率の向上を企図して、核内での遺伝子発現が不要なCRISPR mRNA及びガイドRNAの構築、(3) L-PaCまたはGN-PaCとCRISPR mRNA及びガイドRNAと複合体形成及びそれらの物理化学的性質の評価、(4) ヒト肝実質培養細胞（HepG2細胞）でのゲノム編集効率に関する基礎的検討を行うことを計画した。2019年度は、研究代表者の所属変更に伴う研究環境の大きな変化並びに新型コロナウイルス感染拡大に伴う研究制限等により、計画した研究を十分に遂行できなかった。このような状況から、予算消費も予定額より少なくなった。実際には、ゲノム編集用mRNAの調製の予備的検討として、レポーター遺伝子であるルシフェラーゼmRNAの調製を行った。また、肝実質細胞特異的非ウイルスベクターとして、ラクトース及びPEG修飾PAMAMデンドリマー（G4）の調製を試みた。2019年8月29日～9月1日に中国の成都市で開催された第10回アジアシクロデキストリンシンポジウムにて研究内容の一部を発表した。2020年度は、新型コロナウイルス感染拡大状況に依存するところもあるが、可能な限り研究の遅れを少しでも取り戻すべく尽力したい。, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory), 2019/06 -2022/03, Grant-in-Aid for Challenging Research (Exploratory), Daiichi University, College of Pharmaceutical Sciences - Study on the role of CO as a signaling molecule in cell differentiation
Kitagishi Hiroaki
In this study, to understand the physiological function of carbon monoxide as an intracellular signal molecule, we investigated utilization of the artificial hemoglobin model complex, hemoCD. hemoCD is a supramolecular complex functions as an in vivo CO scavenger. First, the function of hemoCD on cells was examined. As a result, we have established an assay that accurately quantifies intracellular CO using hemoCD. This assay could also be applied to quantify CO in animal tissues, which has filed a patent application. Using this assay, the pharmacokinetics of CO was clarified. Intracellular CO knockdown by a system combining hemoCD and a cell-permeable peptide (R8-hemoCD), development of CORM401-E, a CO-releasing molecule with improved cell-permeability, a heme derivative that does not emit CO when decomposed intracellularly, have been succeeded in developing a group of chemical tools that can be used to elucidate the physiological function of intracellular CO., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2019/06 -2021/03, Grant-in-Aid for Challenging Research (Exploratory), Doshisha University - High-Performance Catalyst and Device Prepared by Metalloporphyrin
林 高史; 大洞 光司; 吉川 佳広; 北岸 宏亮
本研究では、ヘム酸素の活性中心や、生体内での電子移動や酸素分子の運搬・貯蔵をつかさどる補因子のヘム分子(鉄ポルフィリン)の機能に着目し、鉄ポルフィリンに類似した金属ポルフィリン誘導体を基盤とする触媒およびデバイスの創製について、日本の研究グループとフランスの研究グループの各々の分子設計、合成、評価技術を結集して実施する。特に、ポルフィリン分子の骨格変換やシクロデキストリン等を用いた周辺の精密な分子環境設計を施し、金属ポルフィリン類縁体の新規触媒としての利用および、金属ポルフィリン類縁体の集積化に基づくエネルギー・電子移動デバイスの開発を行う。将来的には、分子レベルでの合理的な設計に基づく太陽電池や燃料電池の電極、人工光合成デバイスとして展開可能な基盤技術の獲得をめざす。 当該年度は、日本側の研究者とフランス側の共同研究者の間で、より密な研究討議を行い、それぞれが得意とするポルフィリン・ヘムタンパク質の素材合成を実施した。研究討議のために研究代表者である林はフランスのストラスブールで活動する共同研究者を１１月初旬に訪問し、直接研究討議を行い、今後の方針を立てた。またフランス側が得意とするストラップ部位を導入したポルフィリンについて、日本側が得意とする水中でシクロデキストリンと複合化するための新たなデザインを導入した新規ストラップポルフィリン分子を設計し、その結果を論文として報告した。さらに、光捕集系をめざしたヘム蛋白質集合体の設計・合成にも着手し、幾つかのヘムタンパク質の規則的な複合体の構築と、複合体内での光駆動型のエネルギー移動について、予備的な結果も得た。, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), 2018/10 -2021/03, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Osaka University - Study on the role of endogenous CO in the circadian clock system
Hiroaki Kitagishi
In this study, we investigated the effect of endogenous CO depletion on the circadian clock system using highly selective CO scavenging molecule, hemoCD. Upon injection of hemoCD to mice, the expression levels of clock genes such as Per and Cry were significantly disrupted as a result of CO-depletion. The mechanistic study revealed the activity changes of CO-responsive transcriptional factors, CLOCK and NPAS2. In addition, CO-removal-induced inflammatory responses also caused the disruption of the clock genes. Our study clearly demonstrates, for the first time, that the depletion of endogenous CO induces disruption of circadian clock system. The further mechanistic study is ongoing in our laboratory., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), 2017/04 -2020/03, Grant-in-Aid for Scientific Research (B), Doshisha University - Experimental approach to respiratory and circulatory failure using artificial oxygen carriers and their derivatives
KAWAGUCHI Akira
Hemoglobin-based Oxygen carriers (HBOCs) have been studied to develop alternatives for red blood cell (RBC) transfusion. However, there is no single HBOC that is realized by now. Based on unique physical properties different from RBC, such as nanometer particle size (100-300 nm) and changeable oxygen affinity (P50O2 5-50mmHg), we tested available HBOCs in experimental setting of ischemia/reperfusion in partial or whole brain, myocardium, cochlea and skin ulcer. Accelerated healing of skin ulcer, stomach and bronchus were also noted. Sensitization of cancer radiotherapy or chemotherapy was observed. The mechanisms of action are considered to include, A) ubiquitous O2 as well as CO delivery, B) reduced oxidative stress, C) anti-inflammatory characteristics, D) anti-apoptotic property, resulting in E) preservation of organ/tissue function and morphology at the end. These unique therapeutic properties are considered to be promising and become therapeutics of a totally new category., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), 2015/04 -2020/03, Grant-in-Aid for Scientific Research (A), Tokai University - A stusy on biological function of endogenous CO as a byproduct of heme metabolism
Hiroaki Kitagishi
Carbon monoxide (CO) is continuously produced in mammalian cells during heme metabolic reaction. In this study, we investigated the biological function of the endogenous CO using a selective CO removal agent hemoCD. In order to introduce hemoCD into the cells, we conjugated hemoCD with octaarginine peptide. The new hemoCD derivative (R8-hemoCD) was taken by living cells and captured CO in cells, in which reactive oxygen species level was significantly enhanced in the CO-removed cells. In addition, we newly synthesized a water-soluble biomimetic model for cytochrome c oxidase to investigate the reactivity of CO with a Fe/Cu hetero-binuclear structure of CcO., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, 2016/04 -2018/03, Grant-in-Aid for Challenging Exploratory Research, Doshisha University - Investigation of the role of intracellular CO using a supramolecular heme protein model complex showing high CO affinity
Kitagishi Hiroaki
Carbon monoxide (CO), a well known toxic gas, is continuously produced in the living body. Although the endogenous CO is considered to function in the biological system, the role of CO in biology has not been fully understood. Here, to investigate the biological function of endogenous CO, we utilized a supramolecular heme protein model complex, hemoCD, to remove endogenous CO from the living organisms. HemoCD was capable of quantitatively removing endogenous CO in the mice body due to its extremely high CO affinity. As a result, we succeeded to completely remove CO from the mice body, in which we found a biological feedback mechanism of endogenous CO to compensate the lack of Co in the living organisms (J. Am. Chem. Soc. 2016, 138, 5417-5425)., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), 2014/04 -2016/03, Grant-in-Aid for Young Scientists (B), Doshisha University - Creation of a Supramolecular CO Receptor for Investigation of Biological Roles of Endogenously-Produced Internal CO
KITAGISHI Hiroaki
Carbon monoxide (CO) is continuously produced in the eukaryotic organisms during degradation of hemin by heme oxygenase (HO). The biological roles of CO in the living organisms have not been fully understood because of lacking of knockdown method for endogenously produced CO. We have shown that a supramolecular complex, hemoCD, strongly binds CO higher than that of hemoglobin. In the present study, hemoCD was used for removal of CO in the living organisms and genetic response upon removal of CO was investigated. Furthermore, for monitoring the depletion effect of CO in the human cells, the hemoCD scaffold was conjugated with an octaarginine peptide that has a strong ability to bring molecules into the cell interior. This conjugate, R8-hemoCD, was smoothly taken by the HeLa cells without significant toxicities., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), 2012 -2013, Grant-in-Aid for Young Scientists (B), Doshisha University
Research Seeds
- An Artifi cial Hemoglobin Drug for Toxic Gases Generated in the Fire AccidentsResearch Fields: Medical and Pharmaceutical SciencesSummary: The most toxic gases during combustioon are carbon monoxide (CO) and hydrogen cyanide (HCN). When these two types of gas are inhaled at the same time, they are extremely toxic. In this study, we developed a drug that can remove CO and HCN at the same time.Usage Apply: Emergency medicine at the fire accident place, CO/HCN remover for improving sequelae after the gas poisoning.Remarks: In many cases, HCN and CO are poisoned at the same time in a fi re accident, and there is no method to removeboth at the same time. In particular, HCN poisoning in fi re is not as socially recognized as CO poisoning, despite itshigh lethality. This drug is cost eff ective, stable and convenient, and can remove CO and HCN in a single dose.Patent Information:
特願 2021-170252 - The chemical tools for removal and delivery of carbon monoxide that is a toxic but essential gas in the living organismsResearch Fields: Life Sciences, Chemicals, Nanotechnology and Materials, Medical and Pharmaceutical SciencesSummary: Whereas carbon monoxide (CO) is well known as a toxic gas, CO is continuously produced in our body. CO works as a signal messenger in the living systems, mainly working for cell-protection. In our laboratory, we have been developing a selective CO removal and delivery agents, which are useful for life science research to elucidate the biological function of CO. Similar but different from nitric oxide (NO), CO exerts an unique biological properties and therefore we can use CO as a medicinal tool.Patent Information: 特開2010-194475
特願2019-040610