Researcher Profile and Settings

URL

https://sites.google.com/view/takakuralab/

researchmap

https://researchmap.jp/7000002246

Research Interests

• endurance performance
• mitochondrial biogenesis
• intramuscular oxygen transport mechanism
• muscle tissue
• signal transduction
• hypoxia
• endurance training
• oxidative metabolism

Research Areas

• Life sciences / Physiology
• Life sciences / Sports science / exercise biochemistry

Research Experience

• 同志社大学スポーツ健康科学部 准教授, 2022/04 - Today
• 同志社大学スポーツ健康科学部 助教, 2012/04 - 2022/03
• 日本学術振興会特別研究員（PD）, 2011/04 - 2012/03
• 日本学術振興会特別研究員（DC2）, 2010/04 - 2011/03

Education

• Kanazawa University, Graduate School of Natural Science and Technology, Division of Life Sciences, 2008/04 - 2011/03
• Kanazawa University, Graduate School of Education, 保健体育専攻, 2006/04 - 2008/03
• Kanazawa University, Faculty of Education, スポーツ科学課程, 2002/04 - 2006/03

Association Memberships

• 日本温泉気候物理医学会
• 呼吸研究会
• 日本運動生理学会
• 日本体力医学会
• ヨーロッパスポーツ科学会議（ECSS）
• アメリカ生理学会（APS）
• アメリカスポーツ医学会（ACSM）

Awards

• the Best Paper Award at the annual meeting of the Japanese Society Physical Fitness and Sorts Medicine
  Sep. 2023, 日本体力医学会, Muscle Immobilization delays abrupt change in myoglobin saturation at onset of muscle contraction.
• 大塚スポーツ医･科学賞 特別賞, 第73回日本体力医学会大会（アネッサ・パピリン@福井県福井市）2018.09.07-09., 受賞者: 加藤久詞 他5名
  Sep. 2018
• 若手研究奨励賞, 京都滋賀体育学会第145回大会（京都）2016. 3., 受賞者: 加藤久詞 他3名
  2016, Japan
• 若手研究奨励賞, 京都滋賀体育学会第145回大会（京都）, 受賞者: 高倉久志 他8名
  2016, Japan
• 北陸体育学会優秀研究奨励賞, 平成23年度北陸体育学会大会（金沢）受賞者：蔭地野稔 他5名
  2012, Japan
• 学長表彰, 平成23年3月金沢大学学位記授与式（金沢）受賞者: 高倉久志
  2011, Japan
• 北陸体育学会優秀研究奨励賞, 平成21年度北陸体育学会大会（金沢）受賞者：西河和寛 他8名
  2010, Japan
• YMSFスポーツチャレンジ助成・特別チャレンジャー賞, 平成21年度ヤマハ発動機スポーツ振興財団スポーツチャレンジ助成修了式（掛川）受賞者：増田和実 他2名
  2010, Japan
• 日本運動生理学会奨励賞, 第18回日本運動生理学会大会（鹿児島）受賞者：古市泰郎 他5名
  2010, Japan
• American College of Sports Medicine, Noninvasive Investigations of the Neuromuscular System Interest Group Award, American College of Sports Medicine 56th Annual Meeting（Seattle, WA, USA）受賞者: 高倉久志 他3名
  2009
• 日本体力医学会新潟大会研究奨励賞, 第64回日本体力医学会大会（新潟）受賞者: 高倉久志 他4名
  2009, Japan
• 北陸体育学会優秀研究奨励賞, 平成19年度北陸体育学会大会（金沢）受賞者：古市泰郎 他7名
  2008, Japan
• 日本運動生理学会 The Best Young Investigator Award（若手研究者賞最優秀賞）, 第16回日本運動生理学会大会（奈良）受賞者：増田和実, 高倉久志
  2008, Japan

Published Papers

• High-intensity interval training enhances mRNA expression of IGF1Ea in rat Achilles tendon
  Yoshifumi Tsuchiya; Hisashi Takakura; Seita Osawa; Tetsuya Izawa
  Molecular Biology Reports, Springer Science and Business Media LLC, 51(1), 29 Feb. 2024, Scientific journal
  

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• Impact of high-intensity interval training on tendon related gene expression in rat Achilles tendon
  Yoshifumi Tsuchiya; Hisashi Takakura; Seita Osawa; Tetsuya Izawa
  Biochemical and Biophysical Research Communications, Elsevier BV, Mar. 2023, Scientific journal
  

  DOI URL
• Muscle immobilization delays abrupt change in myoglobin saturation at onset of muscle contraction
  Hisashi Takakura; Tatsuya Yamada; Yasuro Furuichi; Takeshi Hashimoto; Satoshi Iwase; Thomas Jue; Kazumi Masuda
  The Journal of Physical Fitness and Sports Medicine, The Japanese Society of Physical Fitness and Sports Medicine, 11(2) 87 - 96, 25 Mar. 2022, Scientific journal
  

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• 発育期における骨格筋ミトコンドリアの生合成とミオグロビン発現量の連関.
  蔭地野稔; 山田達也; 小間陸嗣; 芝口翼; 高倉久志; 増田和実
  北陸スポーツ・体育学研究, 2 17 - 28, Mar. 2021, Research institution
• Localization of myoglobin in mitochondria: implication in regulation of mitochondrial respiration in rat skeletal muscle.
  Rikuhide Koma; Tsubasa Shibaguchi; Claudia Pérez López; Toshihiko Oka; Thomas Jue; Hisashi Takakura; Kazumi Masuda
  Physiological reports, 9(5) e14769 , Mar. 2021, Scientific journal
  

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• Homeobox A5 and C10 genes modulate adaptation of brown adipose tissue during exercise training in juvenile rats.
  Hisashi Kato; Takuto Ario; Toshiaki Kishida; Manami Tadano; Seita Osawa; Yuki Maeda; Hisashi Takakura; Tetsuya Izawa
  Experimental physiology, 106(2) 463 - 474, Feb. 2021, Scientific journal
  

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• Metabolomic Profiles in Adipocytes Differentiated from Adipose-Derived Stem Cells Following Exercise Training or High-Fat Diet.
  Seita Osawa; Hisashi Kato; Yuki Maeda; Hisashi Takakura; Junetsu Ogasawara; Tetsuya Izawa
  International journal of molecular sciences, 22(2), 19 Jan. 2021, Scientific journal
  

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• Effect of hypoxia exposure after endurance training on muscle oxidative metabolism(和訳中)
  Takakura Hisashi; Takahashi Kazuya; Sudo Mizuki; Ando Soichi; Kato Hisashi; Oosawa Seita; Izawa Tetsuya
  The Journal of Physical Fitness and Sports Medicine, (一社)日本体力医学会, 9(6) 324 - 324, Nov. 2020
• Exercise Training-Enhanced Lipolytic Potency to Catecholamine Depends on the Time of the Day
  Hisashi Kato; Junetsu Ogasawara; Hisashi Takakura; Ken Shirato; Takuya Sakurai; Takako Kizaki; Tetsuya Izawa
  International Journal of Molecular Sciences, MDPI AG, 21(18) 6920 - 6920, 21 Sep. 2020, Scientific journal
  

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• Effects of physical activity and melatonin on brain-derived neurotrophic factor and cytokine expression in the cerebellum of high-fat diet-fed rats.
  Ai Sugiyama; Hisashi Kato; Hisashi Takakura; Seita Osawa; Yuki Maeda; Tetsuya Izawa
  Neuropsychopharmacology reports, 18 Jul. 2020, Scientific journal
  

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• 地域在住高齢者を対象としたコグニサイズとクロレラ摂取の認知機能への効果検証
  野村 友哉; 高倉 久志; 伊藤 祐希; 佐藤 健; 大石 寛; 山口 寛基; 青木 拓巳; 藤島 雅基; 奥村 衣梨; 石井 好二郎
  体力科学, (一社)日本体力医学会, 69(3) 296 - 296, Jun. 2020
• 高齢者を対象としたコグニサイズとクロレラ摂取が認知機能に及ぼす影響およびメタボローム解析による網羅的解析
  野村 友哉; 高倉 久志; 伊藤 祐希; 佐藤 健; 大石 寛; 青木 拓巳; 藤島 雅基; 山口 寛基; 石井 好二郎
  健康支援, 日本健康支援学会, 22(1) 69 - 69, Feb. 2020
• Effect of Short-Term Aerobic Exercise Training on Adropin Levels in Obese Rats.
  Hieda M; Takakura H; Komine H
  J Exer Physiol, 22(7) 64 - 71, Dec. 2019, Scientific journal
• Mitochondrial Nature Adjusted to Host Cellular Demand and Environment.
  Yamada T; Takakura H; Masuda K
  Adv Exer Sport Physiol, 25(1) 9 - 14, Jul. 2019
• Involvement of Myoglobin in Intracellular Oxygen Transport and Oxidative Metabolism.
  Takakura H; Yamada T; Masuda K
  Adv Exer Sport Physiol, 25(1) 1 - 8, Jul. 2019
• Effect of a 9-week exercise training regimen on expression of developmental genes related to growth-dependent fat expansion in juvenile rats
  Hisashi Kato; Takuya Shibahara; Nazibur Rahman; Hisashi Takakura; Yoshinobu Ohira; Tetsuya Izawa
  Physiological Reports, 6(19), Sep. 2018, Scientific journal
  

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• The Effects of Foot Baths on Energy Consumption
  NAKAMURA Masatoshi; TOUDOH Moe; EBINE Naoyuki; FUKUOKA Yoshiyuki; TAKAKURA Hisashi; HOJO Tatsuya
  The Journal of The Japanese Society of Balneology, Climatology and Physical Medicine, The Japanese Society of Balneology, Climatology and Physical Medicine, 81(2) 70 - 75, Aug. 2018, Scientific journal
  

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• Differential response of adipose tissue gene and protein expressions to 4- and 8-week administration of β-guanidinopropionic acid in mice
  Hisashi Kato; Shinya Masuda; Tomotaka Ohira; Luna Ohira; Hisashi Takakura; Yoshinobu Ohira; Tetsuya Izawa
  Physiological Reports, 6(5), Mar. 2018, Scientific journal
  

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• 運動器温熱療法のエビデンスを求めて.
  北條達也; 田中誠智; 高倉久志; 藤堂萌; 海老根直之
  日本生体電気･物理刺激研究会誌, 日本生体電気・物理刺激研究会, 31 11 - 20, Nov. 2017
  

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• Intracellular oxygen tension limits muscle contraction-induced change in muscle oxygen consumption under hypoxic conditions during Hb-free perfusion
  Hisashi Takakura; Minoru Ojino; Thomas Jue; Tatsuya Yamada; Yasuro Furuichi; Takeshi Hashimoto; Satoshi Iwase; Kazumi Masuda
  Physiological Reports, 5(2) e13112 , Jan. 2017, Scientific journal
  

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• Myoglobin and the regulation of mitochondrial respiratory chain complex IV
  Tatsuya Yamada; Hisashi Takakura; Thomas Jue; Takeshi Hashimoto; Rie Ishizawa; Yasuro Furuichi; Yukio Kato; Nobumasa Iwanaka; Kazumi Masuda
  Journal of Physiology, 594(2) 483 - 495, 15 Jan. 2016, Scientific journal
  

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• Endurance training facilitates myoglobin desaturation during muscle contraction in rat skeletal muscle
  Hisashi Takakura; Yasuro Furuichi; Tatsuya Yamada; Thomas Jue; Minoru Ojino; Takeshi Hashimoto; Satoshi Iwase; Tatsuya Hojo; Tetsuya Izawa; Kazumi Masuda
  Scientific Reports, 5 9403 , 24 Mar. 2015, Scientific journal
  

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• 経穴圧痛による長距離走選手の疲労評価の有用性
  谷口 剛志; 加藤 久詞; 高倉 久志; 井口 順太; 北條 達也
  体力科学, (一社)日本体力医学会, 63(6) 685 - 685, Dec. 2014
• Age-induced muscle atrophy and increase in fatigue resistance
  Shinya Masuda; Hisashi Takakura; Hisashi Kato; Tetsuya Izawa
  The Journal of Physical Fitness and Sports Medicine, The Japanese Society of Physical Fitness and Sports Medicine, 3(4) 435 - 439, 2014, Scientific journal
  

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• Interaction between myoglobin and mitochondria in rat skeletal muscle
  Tatsuya Yamada; Yasuro Furuichi; Hisashi Takakura; Takeshi Hashimoto; Yoshiteru Hanai; Thomas Jue; Kazumi Masuda
  Journal of Applied Physiology, 114(4) 490 - 497, 15 Feb. 2013, Scientific journal
  

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• Role of myoglobin in regulating respiration during muscle contraction
  MASUDA Kazumi; YAMADA Tatsuya; ISHIZAWA Rie; TAKAKURA Hisashi
  JPFSM, The Japanese Society of Physical Fitness and Sports Medicine, 2(1) 9 - 16, 2013
  

  DOI URL
• Effect Of Endurance Training On The O-2 Release Rate And Localization Of Myoglobin
  Hisashi Takakura; Tatsuya Yamada; Yasuro Furuichi; Minoru Ojino; Nobumasa Iwanaka; Satoshi Iwase; Thomas Jue; Kazumi Masuda
  JOURNAL OF GENERAL INTERNAL MEDICINE, SPRINGER, 27 388 - 388, Jul. 2012
• Effects Of Endurance Training And Immobilization On The Localization Of Myoglobin In Skeletal Muscle
  Hisashi Takakura; Tatsuya Yamada; Yasuro Furuichi; Minoru Ojino; Nobumasa Iwanaka; Thomas Jue; Kazumi Masuda
  MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, LIPPINCOTT WILLIAMS & WILKINS, 44 349 - 349, May 2012
• Muscle contraction increases carnitine uptake via translocation of OCTN2
  Yasuro Furuichi; Tomoko Sugiura; Yukio Kato; Hisashi Takakura; Yoshiteru Hanai; Takeshi Hashimoto; Kazumi Masuda
  Biochemical and Biophysical Research Communications, 418(4) 774 - 779, 24 Feb. 2012, Scientific journal
  

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• Muscle Contraction Increases Carnitine Uptake Via The Cellular Redistribution Of OCTN2
  Yasuro Furuichi; Tomoko Sugiura; Yukio Kato; Hisashi Takakura; Yoshiteru Hanai; Kazumi Masuda
  MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, LIPPINCOTT WILLIAMS & WILKINS, 43(5) 808 - 808, May 2011
  

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• Effect Of Endurance Training On The O-2 Release Rate And Localization Of Myoglobin
  Hisashi Takakura; Tatsuya Yamada; Yasuro Furuichi; Minoru Ojino; Nobumasa Iwanaka; Satoshi Iwase; Thomas Jue; Kazumi Masuda
  MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, LIPPINCOTT WILLIAMS & WILKINS, 43(5) 388 - 388, May 2011
  

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• Effects of muscle temperature on muscle oxygenation kinetics during contraction.
  Takakura H; Yamada T; Furuichi Y; Iwanaka N; Masuda K
  北陸体育学会紀要, 47 11 - 19, 2011
• Quantification of myoglobin deoxygenation and intracellular partial pressure of O2 during muscle contraction during haemoglobin-free medium perfusion
  Hisashi Takakura; Kazumi Masuda; Takeshi Hashimoto; Satoshi Iwase; Thomas Jue
  Experimental Physiology, 95(5) 630 - 640, May 2010, Scientific journal
  

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• NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle
  Kazumi Masuda; Hisashi Takakura; Yasuro Furuichi; Satoshi Iwase; Thomas Jue
  Advances in Experimental Medicine and Biology, 662 323 - 328, 2010, International conference proceedings
  

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• 筋収縮に伴う細胞内外の酸素環境変化と細胞内酸素ヘムタンパクの存在意義
  高倉久志; 増田和実
  北陸体育学会紀要, 46 1 - 12, 2010
• 音楽刺激が安静時の心臓血管系の応答に及ぼす影響
  渡邊高由樹; 徳田伸一; 高倉久志; 古市泰郎; 嶋田裕哉; 加登万裕子; 増田和実
  45 1 - 8, 2009
• Estimation of Physical Intensity in Uphill and Downhill Walking
  KATO Mayuko; SHIMADA Yuya; FURUICHI Yasuro; TAKAKURA Hisashi; MASUDA Kazumi
  Journal of exercise and sports physiology, 日本運動生理学会事務局, 16(1) 9 - 16, 2009
• Effect of intensive interval training during unloading on muscle deoxygenation kinetics
  Y. Furuichi; K. Masuda; H. Takakura; N. Hotta; K. Ishida; K. Katayama; S. Iwase; H. Akima
  International Journal of Sports Medicine, 30(8) 563 - 568, 2009, Scientific journal
  

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• スポーツタイツの着用が自転車運動時の筋内酸素動態に及ぼす影響.
  高倉久志; 増田和実; 長谷川慎
  北陸体育学会紀要, 43 29 - 36, 2007
• 筋収縮開始時における骨格筋deoxygenationの線形評価.
  高嶋龍也; 増田和実; 高倉久志; 古市泰郎
  北陸体育学会紀要, 42 1 - 7, 2006

MISC

• The influence of endurance training on content of myoglobin localized to muscle mitochondria
  KOMA Rikuhide; SHIBAGUCHI Tsubasa; PEREZ-LOPEZ Claudia; TAKAKURA Hisashi; IZAWA Tetsuya; OKA Toshihiko; MASUDA Kazumi
  Journal of Physical Fitness and Sports Medicine, 9(6), 2020
• Interaction of myoglobin with inner-mitochondria in skeletal muscle
  MASUDA Kazumi; SHIBAGUCHI Tsubasa; PEREZ-LOPEZ Claudia; TAKAKURA Hisashi
  Journal of Physical Fitness and Sports Medicine, 7(6), 2018
• 時計遺伝子の発現リズムに基づいた運動トレーニングが脂肪分解反応に及ぼす影響
  加藤久詞; 田中剛貴; 増田慎也; 高倉久志; 小笠原準悦; 櫻井拓也; 木崎節子; 大野秀樹; 井澤鉄也
  体力科学, 64(6) 665 , 01 Dec. 2015
  

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• PGC‐1αの発現抑制がミオグロビン発現量に及ぼす影響について
  高倉久志; 田中誠智; 田中剛貴; 加藤久詞; 柴原拓哉; 増田慎也; 岩中伸壮; 北條達也; 稗田睦子; 井澤鉄也; 増田和実
  体力科学, (一社)日本体力医学会, 63(6) 571 - 571, Dec. 2014
  

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• Pgc-1 alpha Expression Is Not Essential For Endurance Training-induced Increase In Myoglobin Expression In Skeletal Muscle
  Hisashi Takakura; Goki Tanaka; Seichi Tanaka; Hisashi Kato; Takuya Shibahara; Shinya Masuda; Nobumasa Iwanaka; Toshimichi Hata; Tetsuya Izawa; Thomas Jue; Kazumi Masuda
  MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, LIPPINCOTT WILLIAMS & WILKINS, 46(5) 758 - 758, May 2014, Summary international conference
  

  DOI URL
• 1D04-1 Creatine analogue reduces adipose tissue mass via increased secretion of myostatin(The Proceedings of the 22nd Annual Meetings of Japan Society of Exercise and Sports Physiology July 19・20, (Okayama)) :
  Masuda Shinya; Kato Hisashi; Ohira Yoshinobu; Takakura Hisashi; Izawa Tetsuya
  Advances in exercise and sports physiology, Japan Society of Exercise and Sports Physiology, 20(4) 96 - 96, 2014
• カフェインがミオグロビンタンパク質合成に及ぼす影響
  岩中伸壮; 石澤里枝; 山田達也; 高倉久志; 橋本健志; 横川拓海; 増田和実
  体力科学, (一社)日本体力医学会, 62(6) 521 - 521, Dec. 2013
  

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• 持久的トレーニングが筋細胞内酸素運搬タンパクを増加させる分子機序の解明
  高倉久志; 増田慎也; 加藤久詞; 田中剛貴; 岩中伸壮; 井澤鉄也; 増田和実
  体力科学, (一社)日本体力医学会, 62(6) 578 - 578, Dec. 2013
• Muscle Oxygen Consumption Is Regulated By Oxygen Gradient During Contraction With Hypoxic Perfusion
  Minoru Ojino; Hisashi Takakura; Thomas Jue; Satoshi Iwase; Kazumi Masuda
  JOURNAL OF GENERAL INTERNAL MEDICINE, SPRINGER, 27 388 - 388, Jul. 2012, Summary international conference
• Interaction Of Mitochondria And Oxygen Binding Protein In Skeletal Muscles
  Tatsuya Yamada; Yasuro Furuichi; Hisashi Takakura; Takeshi Hashimoto; Yoshiteru Hanai; Thomas Jue; Kazumi Masuda
  JOURNAL OF GENERAL INTERNAL MEDICINE, SPRINGER, 27 309 - 309, Jul. 2012, Summary international conference
• 身体活動水準の変化がミオグロビンとミトコンドリアの共局在性に及ぼす影響について
  高倉 久志; 古市 泰郎; 山田 達也; 岩中 伸壮; 蔭地野 稔; 岩瀬 敏; 増田 和実
  体力科学, (一社)日本体力医学会, 60(6) 656 - 656, Dec. 2011
• 骨格筋培養細胞を用いたカルニチン取り込み活性調節機序の検証
  古市泰郎; 岩中伸壮; 高倉久志; 山田達也; 橋本健志; 増田和実
  体力科学, (一社)日本体力医学会, 60(6) 689 - 689, Dec. 2011
  

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• Muscle Oxygen Consumption Is Regulated By Oxygen Gradient During Contraction With Hypoxic Perfusion
  Minoru Ojino; Hisashi Takakura; Thomas Jue; Satoshi Iwase; Kazumi Masuda
  MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, LIPPINCOTT WILLIAMS & WILKINS, 43(5) 388 - 388, May 2011, Summary international conference
• Interaction Of Mitochondria And Oxygen Binding Protein In Skeletal Muscles
  Tatsuya Yamada; Yasuro Furuichi; Hisashi Takakura; Takeshi Hashimoto; Yoshiteru Hanai; Thomas Jue; Kazumi Masuda
  MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, LIPPINCOTT WILLIAMS & WILKINS, 43(5) 309 - 309, May 2011, Summary international conference
• カルニチンの細胞内輸送の促進が骨格筋の持久性能力に及ぼす影響
  増田 和実; 古市 泰郎; 高倉 久志; 花井 淑晃; 橋本 健志; 狩野 豊
  健康医科学研究助成論文集, (公財)明治安田厚生事業団, (26) 102 - 109, Mar. 2011
• 2-C-05 Effect of myoglobin functional blockade on the interaction of myoglobin with mitochondria(The Proceedings of the 19th Annual Meetings of Japan Society of Exercise and Sports Physiology August 25・26, (Tokushima)) :
  YAMADA Tatsuya; FURUICHI Yasuro; TAKAKURA Hisashi; OJINO Minoru; MASUDA Kazumi
  Advances in exercise and sports physiology, Japan Society of Exercise and Sports Physiology, 17(2) 77 - 77, 2011
• 2-C-06 Contribution of myoglobin-mediated oxygen delivery to oxygen uptake response at the onset of muscle contraction in hypoxia(The Proceedings of the 19th Annual Meetings of Japan Society of Exercise and Sports Physiology August 25・26, (Tokushima)) :
  OJINO Minoru; TAKAKURA Hisashi; MASUDA Kazumi
  Advances in exercise and sports physiology, Japan Society of Exercise and Sports Physiology, 17(2) 78 - 78, 2011
• 1-B-08 Contraction-induced change in subcellular localization of carnitine transporter(OCTN2)(The Proceedings of the 19th Annual Meetings of Japan Society of Exercise and Sports Physiology August 25・26, (Tokushima)) :
  FURUICHI Yasuro; TAKAKURA Hisashi; YAMADA Tatsuya; MASUDA Kazumi
  Advances in exercise and sports physiology, Japan Society of Exercise and Sports Physiology, 17(2) 59 - 59, 2011
• 筋細胞内におけるミトコンドリアと酸素供給タンパクの共局在
  山田 達也; 古市 泰郎; 橋本 健志; 花井 淑晃; 高倉 久志; 岩中 伸壮; 増田 和実
  体力科学, (一社)日本体力医学会, 59(6) 630 - 630, Dec. 2010
• 持久的トレーニングが筋収縮中の細胞内酸素運搬メカニズムに及ぼす影響について
  高倉 久志; 古市 泰郎; 山田 達也; 岩中 伸壮; 岩瀬 敏; 増田 和実
  体力科学, (一社)日本体力医学会, 59(6) 728 - 728, Dec. 2010
• 1A-O-6 Localization and fiber type specific expression of carnitine/organic cation transporter (OCTN2) in skeletal muscles(The Proceedings of the 18th Annual Meetings of Japan Society Exercise and Sports Physiology Jury 31 August 1, (Kagoshima)) :
  FURUICHI Yasuro; TAKAKURA Hisashi; YAMADA Tatsuya; HANAI Yoshiteru; HASHIMOTO Takeshi; MASUDA Kazumi
  Advances in exercise and sports physiology, Japan Society of Exercise and Sports Physiology, 16(2) 45 - 45, 2010
• 1A-O-4 The effect of endurance training on the intracellular oxygen release rate from myoglobin at the onset of muscle contraction(The Proceedings of the 18th Annual Meetings of Japan Society Exercise and Sports Physiology Jury 31 August 1, (Kagoshima)) :
  TAKAKURA Hisashi; MASUDA Kazumi
  Advances in exercise and sports physiology, Japan Society of Exercise and Sports Physiology, 16(2) 44 - 44, 2010
• 1-P-2 Co-localization of mitochondria and oxygen binding proteins in skeletal muscles(The Proceedings of the 18th Annual Meetings of Japan Society Exercise and Sports Physiology Jury 31 August 1, (Kagoshima)) :
  YAMADA Tatsuya; FURUICHI Yasuro; TAKAKURA Hisashi; HASHIMOTO Takeshi; HANAI Yoshiteru; MASUDA Kazumi
  Advances in exercise and sports physiology, Japan Society of Exercise and Sports Physiology, 16(2) 61 - 61, 2010
• 1-O-30 The effect of immobilization on the intracellular O_2 dynamics during muscle contraction(The Proceedings of the 17th Annual Meetings of Japan Society of Exercise and Sports Physiology Jury 25-26, (Tokyo)) :
  TAKAKURA Hisashi; MASUDA Kazumi
  Advances in exercise and sports physiology, Japan Society of Exercise and Sports Physiology, 15(2) 63 - 63, 2009
• 156. 筋収縮時における細胞内酸素分圧の変化(呼吸・循環,一般口演,第63回日本体力医学会大会)
  高倉久志; 増田和実; 岩瀬敏
  体力科學, 日本体力医学会, 57(6) 710 - 710, 01 Dec. 2008
• 2. Detection of intracellular O_2 dynamics during muscle contraction
  MASUDA KAZUMI; TAKAKURA HISASHI; FURUICHI YASURO
  Japanese Journal of Physical Fitness and Sports Medicine, The Japanese Society of Physical Fitness and Sport Medicine, 57(4) 513 - 513, 01 Aug. 2008
• 1P-Y-20 Immediate decline of intracellular oxygenation at onset of contraction and its relation to muscle oxygen demand(The Proceedings of the 16th Annual Meetings of Japan Society of Exercise and Sports Physiology August 2-3, (Nara)) :
  Masuda Kazumi; Takakura Hisashi
  Advances in exercise and sports physiology, Japan Society of Exercise and Sports Physiology, 14(4) 106 - 106, 2008
• 125. 片脚サスペンジョン中のインターバルトレーニングが下肢筋酸素動態に及ぼす影響(呼吸・循環,第62回日本体力医学会大会)
  古市 泰郎; 増田 和実; 高倉 久志; 堀田 典生; 石田 浩司; 片山 敬章; 秋間 広
  体力科學, 日本体力医学会, 56(6) 641 - 641, 01 Dec. 2007
• Effect of Muscle Temperature on the Oxygenation Kinetics During Contraction
  Hisashi Takakura; Yasuro Furuichi; Kazumi Masuda
  MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, LIPPINCOTT WILLIAMS & WILKINS, 38(5) S224 - S224, May 2006, Summary international conference
• Faster Rate Of Tissue Oxygen Extraction During Contraction After Short Period Of Resistance Training
  Kazumi Masuda; Hisashi Takakura; Hiroshi Akima; Ryuta Kinugasa; Thomas Jue
  MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, LIPPINCOTT WILLIAMS & WILKINS, 37 S390 - S391, May 2005, Summary international conference

Books etc

• 図解スポーツ健康科学入門
  北條, 達也(リハビリテーション科学)
  金芳堂, Apr. 2020
• 身体運動と呼吸・循環機能
  真興交易（株）医学部出版部, 2012

Research Projects

• 運動療法が影響する細胞外マトリックスのダイナミクスに制御される脂肪由来幹細胞機能
  井澤 鉄也; 高倉 久志; 土屋 吉史
  日本学術振興会, 科学研究費助成事業, 2023/04 -2026/03, 基盤研究(B), 同志社大学
• 骨格筋振動刺激はストレスから脳機能を守れるか？間質液に着目した新たな予防法の確立
  須藤 みず紀; 高倉 久志; 安藤 創一; 狩野 豊
  日本学術振興会, 科学研究費助成事業, 2022/04 -2026/03, 基盤研究(B), 公益財団法人明治安田厚生事業団体力医学研究所
• 有酸素性代謝能力を効果的に向上させる低酸素環境を利用した持久的トレーニングの探索
  高倉 久志
  持久的競技パフォーマンスを向上させるための低酸素環境を用いた最適な運動トレーニング（TR）方法についてはその解明に至っていない。これまでの研究において、低酸素暴露かTRを単一の刺激因子として用いた場合は、持久的競技パフォーマンスに関与する別の要因（低酸素刺激の場合は細胞外からの酸素供給能力、TRの場合は細胞内酸素利用能力）をそれぞれ向上させるものの、低酸素暴露とTRの併用はそういった適応を消失させた。そこで本研究では低酸素環境とTRを併用した場合に持久的競技パフォーマンスに関わる要因の向上を抑制した原因を明らかにするとともに、低酸素環境を用いた新たなTR方法についても検証することを目的として、当該年度では、筋組織や腎臓を対象に、どの程度の酸素濃度、どの程度の低酸素暴露時間によって低酸素応答が生じるのかを検討した。 被験動物には9週齢のWistar系雄性ラットを用いた。低酸素暴露時間の条件として1, 3, 6, 24時間と対照群を含む5条件を設定し（この際の酸素濃度は12.0%O2とした）、暴露させる低酸素濃度条件として20.9%O2, 17.7%O2, 15.4%O2, 12%O2の4条件を設定し（この際の暴露時間は3時間とした）、それぞれの刺激条件に対する筋有酸素性代謝に関わる因子のmRNA応答について検証した。その結果、筋組織においては最も条件の厳しい12% O2での暴露であっても24時間までの暴露時間では上記因子のmRNA応答に顕著な変化は認められなかった。その一方で、腎臓では赤血球産生に重要なエリスロポエチンのmRNA発現が、12%O2の低酸素環境下であれば3時間以上の暴露によって増加した。したがって、短時間で十分な低酸素応答を引き起こすためには、少なくとも12%O2よりも厳しい酸素濃度条件で少なくとも3時間の暴露時間を設ける必要があることが示唆された。, 日本学術振興会, 科学研究費助成事業, 2021/04 -2024/03, 基盤研究(C), 同志社大学
• Validation of a training program for efficient acquisition of specific adaptations by hypoxic training
  Takakura Hisashi
  To devise a practical exercise training (TR) program for improving athletic endurance performance in a hypoxic environment, we examined the effects of a combination of hypoxic stimulation and TR. The effects of a protocol of brief hypoxic exposure after endurance exercise on the aerobic metabolic capacity were examined to devise a TR program that would provide a hypoxic environment-specific adaptation for oxygen utilization capacity while maintaining exercise TR. The findings suggest that hypoxic exposure and TR, when used as individual stimulants, improve different factors involved in aerobic metabolic capacity, while the combination of the two factors may attenuate or eliminate their effects., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A), 2017/04 -2021/03, Competitive research funding, Grant-in-Aid for Young Scientists (A), Doshisha University
• Effect of combination of endurance training and anti-inflammatory/antioxidant administration on mitochondrial biogenesis in skeletal muscle
  Takakura Hisashi
  This study examined the effects of a combination of exercise training and anti-inflammatory/antioxidant administration on physical characteristics and mitochondrial biogenesis in a high fat diet-induced obese animal model. In the model, the combination of exercise training and melatonin administration resulted in the most significant weight loss as compared to the control group or either individual factor. However, the weight of muscle tissue, adipose tissue, and mitochondria volume did not follow these changes, suggesting that the mechanism of the combination of exercise training and melatonin administration needs to be investigated in further detail., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Challenging Research (Exploratory), 2017/06 -2020/03, Challenging Research (Exploratory), Doshisha University
• Clarification of mechanism(s) underlying exercise training-induced possible change(s) in the differentiation potential of adipose tissue-derived stem cells
  Izawa Tetsuya; KATO Hisashi; TAKAKURA Hisashi; OGASAWARA Junetsu; SAKURAI Takuya
  Both visceral and subcutaneous adipose tissues are composed of several types of cells. Of these cells, adipose tissue-derived stem cells (ADSC) from either visceral or subcutaneous adipose tissue can be differentiated into each fat depot-specific adipocytes. The aim of the present project was to establish the effect of exercise training on the ability of ADSC into adipocytes and other type of cells. The main findings were as follows. First, fat depot-specific differences in developmental genes were found in the expressions, and some of developmental genes expressions were associated with exercise training-induced changes in adipose tissue weight. Second, exercise training affected intracellular metabolism, probably being associated with the changes in the ability of ADSC into mature adipocytes. Finally, high-fat diet impaired the differentiation ability of ADSC into neuron-like cells, but exercise training ameliorated such the high-fat diet-induced impairment of neurogenesis in ADSC., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2016/04 -2019/03, Grant-in-Aid for Scientific Research (B), Doshisha University
• The analysis for mechanism of exercise-induced adipose tissue remodeling on developmental genes
  Kato Hisashi; IZAWA Tetsuya; TAKAKURA Hisashi
  Recent studies indicate that developmental genes are differentially expressed in subcutaneous adipose tissue (scWAT) and visceral adipose tissue (vWAT), and that some of these genes exhibit changes in expressions which closely correlate with the extent obesity and the pattern of fat distribution. The purpose of the current study was to investigate the effect of exercise training (TR) and high-fat diet (HFD) on developmental genes in three depots: inguinal, epididymal, and brown fat from rats. The fat depot-specific differences in mRNA levels of developmental genes were observed in three fat depots. TR and HFD induced the changes in the expression levels of some of these genes, i.e., HoxA5, HoxC9, and HoxC10. Moreover, the depression of HoxC10 in inguinal and brown adipocytes increases the expressions of brown fat genes, Ucp1 and Pgc1α. These data suggest that HoxC10 modulates adaptation of exercise training on adipose tissues., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2016/08 -2018/03, Grant-in-Aid for Research Activity Start-up, Doshisha University
• New mechanism of mitochondrial biogenesis mediated by nutritional substrate in skeletal muscle
  Masuda Kazumi; TAKAKURA HISASHI; HASHIMOTO TAKESHI; IWANAKA NOBUMASA
  Mitochondrial biogenesis in response to exercise is reliant on adaptive changes through potentially key signal for activating mitochondrial biogenesis (eg. activation of SIRT1, AMPK and PGC-1a). Polyphenol curcumin is a natural antioxidant exhibiting a variety of pharmacological activities and therapeutic properties. However, it remains unknown the effect of curcumin and underlying mechanism on skeletal muscle for regulation of mitochondrial biogenesis. The purpose of the present study was to examine the effects of endurance training (eTR) and curcumin treatment on mitochondrial biogenesis and to identify some of key cascade along the cell signaling. The experimental results suggested that that curcumin treatment with eTR regulated mitochondria biogenesis in skeletal muscle through SIRT1 and AMPK -mediated signaling pathway., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2015/04 -2018/03, Grant-in-Aid for Challenging Exploratory Research, Kanazawa University
• New interaction of oxygen binding protein mediating mitochondrial function in skeletal muscle
  Masuda Kazumi; TAKAKURA HISASHI; IWANAKA NOBUMASA; HASHIMOTO TAKESHI; HAYANO TOSHIYA; KATO YUKIO; HANAI YOSHITERU
  The present study focused on physiological interaction of myoglobin (Mb) with mitochondria in myocyte. In order to identify the hypothesis, we established Mb overexpressing cultured cell model. The Mb-overexpressing myocytes consumed oxygen faster and showed higher enzymatic activity especially in activity of complex-IV. The Mb-siRNAs transfection canceled these positive effects observed in Mb-overexpressed myocytes. We further tested about Mb localization by mitochondrial fractionation assay involved serial centrifuges. The Mb was, then, observed in the mitochondrial intermembrane space. These evidences suggest that Mb would be one of the factors underlying the mechanisms to regulate muscle mitochondrial respiration through an interaction with mitochondrial complex., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2014/04 -2017/03, Grant-in-Aid for Scientific Research (B), Kanazawa University
• Response and adaptation of skeletal muscle to endurance training based on circadian rhythms of clock gene expression.
  Takakura Hisashi
  The purpose of this study was to determine whether exercise timing enhanced exercise training (TR)-induced increase in the levels of mitochondrial proteins in skeletal muscles. The treadmill TR for 9 weeks was conducted at two time points (TR@zeitgeber time 0 (ZT0) and TR@ZT10). Both of per2 and pgc-1α mRNA expression showed the highest level at ZT0 and lowest level at ZT10. Additionally, the rats underwent acute exercise at the same time points (Ex@ZT0 and Ex@ZT10). The TR significantly increased levels of the mitochondrial protein (COXIV) in the deep portion of gastrocnemius muscle. Moreover, COXIV level in the TR@ZT10 group increased significantly compared to those in the TR@ZT0 group. Indeed, pgc-1α mRNA expression increased significantly immediately after acute exercise only at Ex@ZT10. The timing of TR influenced TR-induced adaptation. This differential adaptation might be caused by the differential PGC-1α expression upon acute exercise., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), 2014/04 -2017/03, Competitive research funding, Grant-in-Aid for Young Scientists (B), Doshisha University
• Comprehensive analysis for interaction of oxygen binding protein with mitochondrial proteins in skeletal muscle
  MASUDA Kazumi; TAKAKURA Hisahi; IWANAKA Nobumasa; HASHIMOTO Takeshi; HAYANO Toshiya; HANAI Yoshiteru; KATO Yukio; SUGIURA Tomoko
  Cellular respiration depends upon a coordinated response of the cardiovasculature and metabolism. Myoglobin (Mb), expressing specifically in myocytes, has been found in mitochondria. This unique finding has led us to the hypothesis that mitochondrial Mb may have a direct role for the regulation of mitochondrial respiration. In order to test this idea, we determined protein-protein interaction between Mb and mitochondrial proteins in genetically modified C2C12 cell by proteomic analysis, IP and WB. It was confirmed that Flag-tagged Mb localized in mitochondria in the C2C12 cells that express Flag-Mb stably. Proteomic analysis identified several mitochondrial proteins, which interacted with Mb. These proteins detected in IP samples of Mb-Flag, occupied 24% of entire proteins. IP and WB detected both outer and inner membrane proteins (VDAC and COX-IV) from the mitochondrial fraction. These results suggested the potential interaction of mitochondrial proteins with Mb in skeletal myocytes., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2013/04 -2015/03, Grant-in-Aid for Challenging Exploratory Research, Kanazawa University
• Interaction of oxygen carrier protein with mitochondria activity during muscle contraction
  MASUDA Kazumi; TAKAKURA Hisashi; IWANAKA Nobumasa; HASHIMOTO Takeshi; HAYANO Toshiya; HANAI Yoshiteru; KATO Yukio
  Cellular respiration is influenced by a wide range of mechanisms including the response of the cardiovascular and metabolic systems to meet the changing energy demands in muscle. The present focused on myoglobin (Mb), an important cellular O2 binding protein that is expressed in skeletal and cardiac muscle cells, and set a hypothesis that Mb will have a direct interaction with the mitochondrial respiration. The present study using rodent hindlimb muscles and cultured myoblast (L6), obtained evidences that Mb directly co-localized on mitochondria within a myocytes. Unexpectedly, Mb presences in mitochondria. The mitochondrial Mb can interact with complex IV to facilitated O2 consumption in mitochondria. Further investigation must be necessary to clarify the target proteins of Mb within mitochondria to obtain the new physiological functions of the mitochondrial Mb in myocytes., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2011/04 -2014/03, Grant-in-Aid for Scientific Research (B), Kanazawa University
• The elucidation of molecular mechanism for increase in myoglobin expression and the examination of its optimal expression condition.
  TAKAKURA Hisashi
  Endurance exercise training (eTR) improves muscle oxidative capacity via an increase in both mitochondrial protein and myoglobin (Mb). Overexpression of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) increases Mb expression in skeletal muscle. Knockout of PGC-1α, however, does not attenuate eTR-induced increase in Mb expression. Therefore, it is controversial how PGC-1α is involved in eTR-induced increase in Mb expression in skeletal muscle. The purpose of this study was to verify whether an increase in PGC-1α expression in skeletal muscle is required for the eTR-induced increase in Mb expression. Timing of increase in Mb expression due to eTR was different from that of mitochondria protein (COX IV). PGC-1α expression did not show any significant differences during the training protocol in this study. Moreover, the PGC-1α mRNA knockdown by siRNA transfection did not attenuate Mb mRNA expression in C2C12 cells. In conclusion, PGC-1α is not essential for the eTR-induced increase in Mb expression in skeletal muscle., Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity start-up, 2010 -2013, Grant-in-Aid for Research Activity start-up, Doshisha University
• 筋収縮時における細胞内酸素ダイナミクスについて
  高倉 久志
  筋収縮時におけるミオグロビン(Mb)からの酸素供給量は身体活動水準に依存して増減する。トレーニング(Tr)後の骨格筋ではミトコンドリアに共局在するMbの増加が、筋収縮時におけるMbからの酸素供給量の増加に貢献する可能性が示唆された。しかしながら、不活動(IM)後のMbからの酸素供給量が減少した骨格筋において、ミトコンドリアに共局在するMbが減少するか否かについては検討が不十分であった。また、ミトコンドリアとMbの相互作用性を検討するにあたり、当該年度は骨格筋ミトコンドリアの呼吸活性測定にも着手した。そこで本年度は、IMによってミトコンドリアに共局在するMbが減少するか否かと、骨格筋組織からミトコンドリアを単離することによる呼吸活性測定手法の確立とその手法を用いてTrやIMがミトコンドリア呼吸活性に及ぼす影響について検討した。その結果、IM後における骨格筋ミトコンドリア画分のMb発現量に変化は認められなかった。したがって、IM後の骨格筋ではミトコンドリアに共局在するMb量が変化しないことが示唆された。このことは、IM後の筋収縮時におけるMbからの酸素供給量が減少するメカニズムにはミトコンドリアとMbの共局在性が関与していない可能性を示唆するものの、IMの期間や方法などについて更なる検討が必要であろう。また、骨格筋組織からミトコンドリアを単離し、その呼吸活性を測定した。その結果、筋重量あたりのミトコンドリア最大呼吸活性がTr後の骨格筋では上昇傾向を示し、IM後の骨格筋では有意に減少した。今後はMbとミトコンドリアの相互作用性を検討できるようなミトコンドリア呼吸活性の測定方法を考慮に入れながら、更なる検討を進めていく必要があると考えられる。, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows, 2010 -2011, Competitive research funding, Grant-in-Aid for JSPS Fellows, Kanazawa University
• 持久的トレーニングによる筋細胞内酸素運搬タンパクの増加を担う分子機序の解明
  公益財団法人中冨健康科学振興財団, 第24回研究助成金, 各種団体からの研究助成, Competitive research funding
• 持久的運動トレーニング効果を増強する運動実施タイミングの検討
  （財）ヤマハ発動機スポーツ振興財団, 平成28年度チャレンジ研究助成, 各種団体からの研究助成, Competitive research funding